Abstract
The delivery system, using artificial seed, is of great value in germplasm storage, propagation, and in the production of chemicals. Artificial seed for plant propagation has been investigated in somatic embryogenesis (Kitto and Janick 1985; Redenbaugh et al. 1991). We have extended this concept to the hairy root because of the successful regeneration of the whole plant from Ri-transformed cells. The micropropagation system using these hairy roots promises practical application in the fields of cellular biology, agriculture, and bioengineering.
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References
Bajaj YPS (ed) (1993) Biotechnology in agriculture and forestry, vol. 21. Medicinal and aromatic plants IV. Springer, Berlin Heidelberg New York
Kato Y, Uozumi N, Kimura T, Honda H, Kobayashi T (1991) Enhancement of peroxidase production and excretion from horseradish hairy roots by light, NaCI, and peroxidase-adsorption in situ. Plant Tissue Cult Lett 8: 158–165
Kilby NJ, Hunter CS (1990) Repeated harvest of vacuole-located secondary product from in vitro grown plant cells using 1.02 MHz ultrasound. Appl Microbiol Biotechnol 33: 448–451
Kitto SL, Janick J (1985) Production of synthetic seeds by encapsulating asexual embryos of carrot. J Am Soc Hortic Sci 110: 227–282
Kondo O, Honda H, Taya M, Kobayashi T (1989) Comparison of growth properties of carrot hairy root in various bioreactors. Appl Microbiol Biotechnol 33: 291–294
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Noda T, Tanaka N, Mano Y, Nabeshima S, Ohkawa H, Matsui C (1987) Regeneration of horseradish hairy roots incited by Agrobacterium rhizogenes infection. Plant Cell Rep 6: 283–286
Redenbaugh K, Fujii J, Slade D, Viss P, Kossler M (1991) Artificial seeds —Encapsulated somatic embryos. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 17. High-tech and micropropagation I. Springer, Berlin Heidelberg New York, pp 395–416
Shimomura K, Sudo H, Saga H, Kamada H (1991) Shikonin production and secretion by hairy root cultures of Lithospermum erythrorhizon. Plant Cell Rep 10: 282–285
Tanaka N, Hayakawa M, Mano Y, Ohkawa H, Matsui C (1985) Infection of turnip and radish storage roots with Agrobacterium rhizogenes. Plant Cell Rep 4: 74–77
Taya M, Yoyama A, Kondo O, Kobayashi T (1989a) Growth characteristics of plant hairy roots and their cultures in bioreactors. J Chem Eng Jpn 22: 84–89
Taya M, Yoyama A, Nomura R, Kondo O, Matsui C, Kobayashi T (1989b) Production of peroxidase with horseradish hairy root cells in a two step culture system. J Ferment Bioeng 67: 31–34
Taya M, Mine K, Kino-oka M, Tone S, Ichi T (1992) Production and release of pigments by culture of transformed hairy root of red beet. J Ferment Bioeng 73: 31–36
Tepfer D (1990) Genetic transformation using Agrobacterium rhizogenes. Physiol Plant 79: 140–146
Uozumi N, Kohketsu K, Kobayashi T (1993) Plant hairy root growth and metabolism in fed-batch culture on monosaccharide medium. J Chem Technol Biotechnol 57: 155–161
Uozumi N, Asano Y, Kobayashi T (1994) Micropropagation of horseradish hairy root by means of adventitious shoot primordia. Plant Cell Tissue Organ Cult 36: 183–190
Uozumi N, Kohketsu K, Kondo O, Honda H, Kobayashi T (1991) Fed-batch culture of hairy root using fructose as a carbon source. J Ferment Bioeng 72: 457–460
Uozumi N, Yoshihiro K, Nakashimada Y, Kobayashi T (1992a) Excretion of peroxidase from horseradish hairy root in combination with ion supplementation. Appl Microbiol Biotechnol 37: 560 565
Uozumi N, Nakashimada Y, Kato Y, Kobayashi T (1992b) Production of artificial seed from horseradish hairy root. J Ferment Bioeng 74: 21–26
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© 1995 Springer-Verlag Berlin Heidelberg
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Uozumi, N., Kobayashi, T. (1995). Artificial Seed Production Through Encapsulation of Hairy Root and Shoot Tips. In: Bajaj, Y.P.S. (eds) Somatic Embryogenesis and Synthetic Seed I. Biotechnology in Agriculture and Forestry, vol 30. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03091-2_11
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DOI: https://doi.org/10.1007/978-3-662-03091-2_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-08183-5
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