Skip to main content

Method to Assess the Strength of Cell-Cell Adhesion Using a Modified Flow Cytometer

  • Chapter
Studying Cell Adhesion
  • 60 Accesses

Abstract

Cell-cell adhesion embraces a series of phenomena which cover many aspects of biology. They can be best described as a conjunction of morphological, biochemical and physical features. Since the pioneering works on the characterization of the first cell surface molecules involved in cell adhesion of Dictyostelium or nerve cells (Beug 1973; Thiery 1977; Gerisch 1980), the advances of molecular biology techniques have yielded an every-day-increasing catalog of surface molecules involved in so-called adhesion pathways, i.e. receptor/ligand interactions which mediate adhesion.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Amblard F (1993a). Fluid mechanical properties of flow cytometers and assessment of cell-cell adhesion forces. In: Jacquemin-Sablon A and Crissman H (eds) Flow cytometry New Developments. ASI Series, Springer Verlag, Berlin p205

    Chapter  Google Scholar 

  • Amblard F (1993b) Hydrodynamic extensions applied to the assessment of contact forces between suspended particles: a theoretical description. submitted.

    Google Scholar 

  • Amblard F, Cantin, C, Durand J, Fischer A, Sékaly R, ffray C (1992). New chamber for flow cytometric analysis over an extended range of stream velocity and its application to cell adhesion measurements. Cytometry 13: 15–22

    Article  PubMed  CAS  Google Scholar 

  • Amblard F, Auffray C, Sékaly R, Fischer A (1994). Molecular analysis of antigen-independent adhesion forces between T and B lymphocytes. Proc Nat Acad Sci USA 91: 3628–3632

    Article  PubMed  CAS  Google Scholar 

  • Baltz J M, Cone R A (1990). The strength of non-covalent biological bonds and adhesion by multiple independent bonds. J Theor Biol 142: 163–178

    Article  PubMed  CAS  Google Scholar 

  • Batchelor GK (1967). An introduction to fluid dynamics. Cambridge University Press, Cambridge

    Google Scholar 

  • Beug H, Katz FE, Stein A and Gerisch G (1973). Quantification of membrane sites in aggregating “Dictyostelium discoideum” by use of tritiated univalent antibodies. Proc Nat Acad Sci USA 70: 3150–3154

    Article  PubMed  CAS  Google Scholar 

  • Bongrand P (ed) (1988). Physical basis of cell-cell adhesion, CRC Press, Boca Raton

    Google Scholar 

  • Crosland-Taylor PJ (1953). A device for counting small particles suspended in a fluid through a tube. Nature 171: 37–38

    Article  PubMed  CAS  Google Scholar 

  • Gerisch G (1980). Univalent antibody fragments as tools for the analysis of cell interactions in Dictyostelium. Curr Topics Dev Biol 14: 243–249

    Article  CAS  Google Scholar 

  • Happel J, Brenner H (1983). Low Reynolds number hydrodynamics, Kluwer Academic publishers, Dordrecht Boston London, p 96

    Book  Google Scholar 

  • Kachel V, Fellner-Feldegg H, Menke E (1990). Hydrodynamic properties of flow cytometry instruments. In: Melamed MR, Lindmo T, Mendelsohn ML (eds) Flow cytometry and sorting. Wiley and Sons, New York, p 27

    Google Scholar 

  • Melamed MR, Lindmo T, Mendelsohn ML (eds) Flow cytometry and sorting (1990). Wiley and Sons, New York

    Google Scholar 

  • Pinkel D, Stovel R (1985). Flow chambers and sample handling In: Van Dilla M A, Dean P N, Laerum O D, Melamed M R (eds) Flow cytometry: instrumentation and data analysis. Academic press, p 77

    Google Scholar 

  • Schmid-Schönbein G (1990). Mechanical properties of leukocytes. Cell Biophysics 17: 107–135

    PubMed  Google Scholar 

  • Thiery J-P, Brackenbury R, Rutishauser U and Edelman GM (1977). Adhesion among neural cells of the chick embryo. II. Purification and characterization of cell adhesion molecule from neural retina. J Biol Chem 252: 6841–6846

    PubMed  CAS  Google Scholar 

  • Van Dilla M A, Dean P N, Laerum O D, Melamed M R (eds) Flow cytometry: instrumentation and data analysis (1985). Academic press.

    Google Scholar 

  • White FM (1991). Viscous Flows. McGraw-Hill, New York

    Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Amblard, F. (1994). Method to Assess the Strength of Cell-Cell Adhesion Using a Modified Flow Cytometer. In: Bongrand, P., Claesson, P.M., Curtis, A.S.G. (eds) Studying Cell Adhesion. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03008-0_7

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-03008-0_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-03010-3

  • Online ISBN: 978-3-662-03008-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics