Techniques for Studying Blood Platelet Adhesion

  • J. J. Sixma
  • P. G. de Groot


Adhesion of blood platelets to the vessel wall is the first step in the formation of a haemostatic plug or thrombus. Because of this, it is an important and crucial step. Routine techniques for the study of platelet function do not include adhesion studies, partly because no reliable technique is available. Information about adhesive function is sought by indirect means such as aggregation of blood platelets caused by collagen or studies of the von Willebrand factor- Glycoprotein lb interaction via the use of the obsolete antibiotic ristocetin or the snake venom protein botrocetin.


Platelet Adhesion Umbilical Artery Blood Platelet Vessel Segment Wall Shear Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Baumgartner HR, Haudenschild C (1972) Adhesion of platelets to subendothelium. Ann N Y Acad Sci 201: 22–36PubMedCrossRefGoogle Scholar
  2. Baumgartner BR, Muggii R (1976a) Adhesion and aggregation: morphologic demonstration, quantitation in vivo and in vitro. In: Gordon JL (ed) Platelets in Biology and Pathology. North Holland, Amsterdam, 23–60Google Scholar
  3. Baumgartner HR, Muggii R, Tschopp TB, Turitto VT (1976b) Platelet adhesion, release and aggregation in flowing blood: effects of surface properties and platelet function. Thromb Haemost 35: 124–138PubMedGoogle Scholar
  4. Lassila R, Badimon JJ, Vallabhajosula S, Badimon L (1990) Dynamic monitoring of platelet deposition on severely damaged vessel wall in flowing blood. Effects of different stenoses on thrombus growth. Arteriosclerosis 10: 306–315.Google Scholar
  5. Muggli R, Baumgartner HR, Tschopp TB, Keller H (1980) Automated microdensitometry and protein assay as a measure for platelet adhesion and aggregation on collagen-coated slides under controlled flow conditions. J Lab Clin Med 95: 195–207PubMedGoogle Scholar
  6. Nievelstein PFEM, d’Alessio PA, Sixma JJ (1988) Fibronectin in platelet adhesion to human collagen types I and III. Use of nonfibrillar and fibrillar collagen in flowing blood studies. Arteriosclerosis 8: 200–206Google Scholar
  7. Nievelstein PFEM, De Groot PhG, d’Alessio PA, Heijnen HFG, Orlando E, Sixma JJ (1990) Platelet adhesion to vascular cells. The role of exogenous von Willebrand factor in platelet adhesion. Arteriosclerosis 10: 462–469Google Scholar
  8. Piotrowicz RS, Orchekowski RP, Nugent DJ, Yamada KY, Kunicki TJ (1988) Glycoprotein Ic-IIa functions as an activation independent fibronectin receptor on human platelets. J Cell Biol 106: 1359–1364PubMedCrossRefGoogle Scholar
  9. Sakariassen KS, Aarts PAMM, De Groot PhG, Houdijk WPM, Sixma JJ (1983) A perfusion chamber developed to investigate platelet interaction in flowing blood with human vessel wall cells, their extracellular matrix and purified components. J Lab Clin Med 102: 522–535PubMedGoogle Scholar
  10. Sixma JJ, Nievelstein PE,M, Zwaginga JJ, De Groot PhG (1987) Adhesion of blood platelets to the extracellular matrix of cultured human endothelial cells. Ann N Y Acad Sci 516: 39–51Google Scholar
  11. Staatz WD, Rajpara SM, Wayner EA, Carter WG, Santoro SA (1989) The membrane glycoprotein la-IIa (VLA-2) complex mediates the Mg++-dependent adhesion of platelets to collagen. J Cell Biol 108: 1917–1924PubMedCrossRefGoogle Scholar
  12. Tschopp TB, Baumgartner HR (1975) Physiological experiments in hemostasis and thrombosis. Brit J Haematol 31: 221–229CrossRefGoogle Scholar
  13. Weiss HJ, Turitto VT, Vicic WJ, Baumgartner HR (1984) Fibrin formation, fibrinopeptide release and platelet thrombus dimension on subendothelium exposed to flowing native blood: greater in factor XII and XI than in factor VIII and IX deficiency. Blood 63: 1004–1015PubMedGoogle Scholar
  14. Westphal C, Horler H, Pentz S, Frosch D (1988) A new method for cell culture on an electron-transparent melamine foil suitable for successive LM, TEM and SEM studies of whole cells. J Microsc 150: 225–231Google Scholar
  15. Zwaginga JJ, de Boer HC, IJsseldijk MJW, et al (1990a) Thrombogenicity of vascular cells. Comparison between endothelial cells isolated from different sources and smooth muscle cells and fibroblasts. Arteriosclerosis 10: 437–448Google Scholar
  16. Zwaginga JJ, IJsseldijk MJW, Beeser-Visser N, De Groot PhG, Vos J, Sixma JJ. (1990b) High von Willebrand factor concentration compensates a relative adhesion defect in uremic blood. Blood 75: 1498–1507PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • J. J. Sixma
  • P. G. de Groot

There are no affiliations available

Personalised recommendations