Techniques for Studying Blood Platelet Adhesion
Adhesion of blood platelets to the vessel wall is the first step in the formation of a haemostatic plug or thrombus. Because of this, it is an important and crucial step. Routine techniques for the study of platelet function do not include adhesion studies, partly because no reliable technique is available. Information about adhesive function is sought by indirect means such as aggregation of blood platelets caused by collagen or studies of the von Willebrand factor- Glycoprotein lb interaction via the use of the obsolete antibiotic ristocetin or the snake venom protein botrocetin.
KeywordsCatheter Depression Urea Aspirin Prostaglandin
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- Baumgartner BR, Muggii R (1976a) Adhesion and aggregation: morphologic demonstration, quantitation in vivo and in vitro. In: Gordon JL (ed) Platelets in Biology and Pathology. North Holland, Amsterdam, 23–60Google Scholar
- Lassila R, Badimon JJ, Vallabhajosula S, Badimon L (1990) Dynamic monitoring of platelet deposition on severely damaged vessel wall in flowing blood. Effects of different stenoses on thrombus growth. Arteriosclerosis 10: 306–315.Google Scholar
- Nievelstein PFEM, d’Alessio PA, Sixma JJ (1988) Fibronectin in platelet adhesion to human collagen types I and III. Use of nonfibrillar and fibrillar collagen in flowing blood studies. Arteriosclerosis 8: 200–206Google Scholar
- Nievelstein PFEM, De Groot PhG, d’Alessio PA, Heijnen HFG, Orlando E, Sixma JJ (1990) Platelet adhesion to vascular cells. The role of exogenous von Willebrand factor in platelet adhesion. Arteriosclerosis 10: 462–469Google Scholar
- Sixma JJ, Nievelstein PE,M, Zwaginga JJ, De Groot PhG (1987) Adhesion of blood platelets to the extracellular matrix of cultured human endothelial cells. Ann N Y Acad Sci 516: 39–51Google Scholar
- Westphal C, Horler H, Pentz S, Frosch D (1988) A new method for cell culture on an electron-transparent melamine foil suitable for successive LM, TEM and SEM studies of whole cells. J Microsc 150: 225–231Google Scholar
- Zwaginga JJ, de Boer HC, IJsseldijk MJW, et al (1990a) Thrombogenicity of vascular cells. Comparison between endothelial cells isolated from different sources and smooth muscle cells and fibroblasts. Arteriosclerosis 10: 437–448Google Scholar