Low Molecular Weight GTP-binding Proteins in Rough Endoplasmic Reticulum Membranes from Rat Liver and Rat Hepatocellular Carcinomas

  • Joël Lanoix
  • Jacques Paiement
Conference paper
Part of the NATO ASI Series book series (volume 74)


Physiological concentrations of GTP have been shown to stimulate a variety of biochemical and morphological events in rough endoplasmic reticulum (RER) membranes. This includes Ca+ + release, protein translocation, core glycosylation, membrane permeability changes, phospholipid metabolism and membrane fusion. In effort to identify GTP-binding proteins involved in specific RER functions we have looked for similar proteins in a rat liver membrane preparation enriched in derivatives of RER. Furthermore, we have studied the effects of a variety of post-translational modifications of GTP-binding proteins on GTP-dependent membrane fusion.


Rough Endoplasmic Reticulum Rough Microsome Membrane Permeability Change Charge Isomer Similar Electrophoretic Mobility 
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  1. Dominguez JM, Lanoix J, Paiement J (1991) Localization of ras antigenicity in rat hepatocyte plasma membrane and rough endoplasmic reticulum fractions. Exp Cell Res 192: 137–147PubMedCrossRefGoogle Scholar
  2. Lanoix J, Paiement J (1991) cAMP-dependent phosphorylation of RER proteins from rat liver: relationship with GTP-dependent membrane fusion. Biochem Biophys Res Comm 179: 463–470Google Scholar
  3. Lanoix J, Roy L, Paiement J (1989) Detection of GTP-binding proteins in purified derivatives of rough endoplasmic reticulum. Biochem J 262: 497–503PubMedGoogle Scholar
  4. Paiement J, Dominguez JM, Guénette A, Roy L (1991) GTP-dependent membrane fusion during hepatocarcinogenesis and liver regeneration. Biochem Biophys Res Comm 176: 1494–1500PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Joël Lanoix
    • 1
  • Jacques Paiement
    • 1
  1. 1.Département d’anatomieUniversité de MontréalMontréalCanada

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