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Low Molecular Weight GTP-binding Proteins in Rough Endoplasmic Reticulum Membranes from Rat Liver and Rat Hepatocellular Carcinomas

  • Joël Lanoix
  • Jacques Paiement
Conference paper
Part of the NATO ASI Series book series (volume 74)

Abstract

Physiological concentrations of GTP have been shown to stimulate a variety of biochemical and morphological events in rough endoplasmic reticulum (RER) membranes. This includes Ca+ + release, protein translocation, core glycosylation, membrane permeability changes, phospholipid metabolism and membrane fusion. In effort to identify GTP-binding proteins involved in specific RER functions we have looked for similar proteins in a rat liver membrane preparation enriched in derivatives of RER. Furthermore, we have studied the effects of a variety of post-translational modifications of GTP-binding proteins on GTP-dependent membrane fusion.

Keywords

Rough Endoplasmic Reticulum Rough Microsome Membrane Permeability Change Charge Isomer Similar Electrophoretic Mobility 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Dominguez JM, Lanoix J, Paiement J (1991) Localization of ras antigenicity in rat hepatocyte plasma membrane and rough endoplasmic reticulum fractions. Exp Cell Res 192: 137–147PubMedCrossRefGoogle Scholar
  2. Lanoix J, Paiement J (1991) cAMP-dependent phosphorylation of RER proteins from rat liver: relationship with GTP-dependent membrane fusion. Biochem Biophys Res Comm 179: 463–470Google Scholar
  3. Lanoix J, Roy L, Paiement J (1989) Detection of GTP-binding proteins in purified derivatives of rough endoplasmic reticulum. Biochem J 262: 497–503PubMedGoogle Scholar
  4. Paiement J, Dominguez JM, Guénette A, Roy L (1991) GTP-dependent membrane fusion during hepatocarcinogenesis and liver regeneration. Biochem Biophys Res Comm 176: 1494–1500PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Joël Lanoix
    • 1
  • Jacques Paiement
    • 1
  1. 1.Département d’anatomieUniversité de MontréalMontréalCanada

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