Purification of the N-Acetylglucosamine-1-Phosphodiester α-N-Acetylglucosamindase from Human Lymphoblasts

  • Ke-Wei Zhao
  • Arnold L. Miller
Conference paper
Part of the NATO ASI Series book series (volume 74)


The G1cNAc-1-phosphodiester α-N-acetylglucosaminidase (“uncovering enzyme”) plays a key role in the intracellular targeting of lysosomal enzymes by catalyzing the removal of N-acetylglucosamine exposing mannose-6-phosphate residues on high mannose and/or hybrid oligosaccharide chains of lysosomal enzymes. The subcellular localization of the uncovering enzyme has been proposed to be associated with the cis Golgi cisternae, but no definitive evidence exists as to either its exact location or structure.


Lysosomal Enzyme Intracellular Target High Mannose Activity Fold Purify Enzyme Preparation 
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  1. Varki A, Kornfeld S (1981) Purification and characterization of rat liver n-N-acetylglucosaminyl phosphodiesterase. J Biol Chem 256: 9937–9943PubMedGoogle Scholar
  2. Waheed A, Hassilik A, Von Figura K (1981) Processing of the phosphorylated recognition marker in lysosomal enzymes. J Biol Chem 256: 5717–5721PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Ke-Wei Zhao
    • 1
  • Arnold L. Miller
    • 1
  1. 1.Department of Neurosciences, 0624University of California, San DiegeLa JollaUSA

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