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Direct Comparision of the Endocytic Routes of Fluorescently-Labelled Lipids and Transferrin

  • Satyajit Mayor
  • Frederick R. Maxfield
Conference paper
Part of the NATO ASI Series book series (volume 74)

Abstract

Intracellular trafficking of proteins during endocytosis has been extensively characterized. This process is mediated by vesicular carriers such as coated vesicles, sorting endosomes and late endosomes, all of which are composed primarily of membrane proteins and lipid. A central question in the endocytic process concerns the sorting of recycling components from lysosomally-directed components. An iterative fractionation model (Dunn and Maxfield, 1992; Dunn et al., 1989) and other current models of endocytic sorting (Linderman and Lauffenburger, 1988) propose that the geometry of the sorting endosome directs bulk membrane, including membrane-associated receptors, towards the recycling pathway while the volume content, consisting of the acid-released ligands, is lysosomally targetted. However, it is not known whether recycling membrane receptors follow bulk membrane flow or if these proteins are actively sorted from the lysosomally-directed material because of specific cytoplasmic tail sequences. The kinetics of movement of the bulk carrier, membrane lipids, will directly address this issue; a difference in the kinetics of lipid-traffic in relation to protein-traffic will provide evidence for mechanisms of selective protein transport or absence of difference will support the hypothesis that proteins follow ‘bulk’ membrane traffic.

Keywords

Late Endosome Endosomal Compartment Bulk Carrier Recycling Endosome Membrane Traffic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Dunn KW, Maxfield FR (1990) Use of Fluorescence microscopy in the study of receptor-mediated endocytosis. In: Herman B and Jackson K (eds) Optical microscopy for biology, Wiley-Liss, New York, p 153Google Scholar
  2. Dunn KW, Maxfield FR (1992) Delivery of ligands from sorting endosomes to late endosomes occurs by maturation of sorting endosomes. J Cell Biol 117: 301 - 310PubMedCrossRefGoogle Scholar
  3. Dunn KW, McGraw TE, Maxfield FR (1989) Iterative fractionation of recycling receptors from lysosomally destined ligands in an early sorting endosome. J Cell Biol 109: 3303 - 3314PubMedCrossRefGoogle Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Satyajit Mayor
    • 1
  • Frederick R. Maxfield
    • 1
  1. 1.Department of Pathology, College of Physicians and SurgeonsColumbia UniversityNew YorkUSA

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