Endosomes and Cell Signalling
The biological responses to many peptide hormones and growth factors are currently under intense investigation. The main area of uncertainty encompasses the transducing events which link initial events at the cell surface to those processes influencing the proteins (egs. enzymes, transporters) effecting the biological response. We have pursued the study of insulin and EGF action by examining the events following ligand-receptor complex formation at the cell surface. Along with others we have observed that these complexes undergo rapid internalization (Posner et al.,1982; Bergeron et al., 1985). In the case of the EGF and insulin receptor this process is accompanied by activation of their respective receptor tyrosine kinases (Cohen et al., 1985; Kay et al., 1986; Khan et al., 1986; Posner et al., 1987).
KeywordsEpidermal Growth Factor Receptor Insulin Receptor Phosphorylated Epidermal Growth Factor Receptor Insulin Receptor Kinase Cell BioI
- Diment S, Martin KJ, Stahl PD (1989) Cleavage of parathyroid hormone in mactrophage endosomes illustrates a novel pathway for intracellular processing of proteins. J Biol Chem 264: 13403: 13406Google Scholar
- Lai WH, Cameron PH, Doherty II JJ, Posner BI, Bergeron JJM (1989a) Ligand mediated autophosphorylation activity of the EGF receptor during internationalization. J Cell Biol 109: 2751–2760Google Scholar
- Lai WH, Cameron PH, Wada I, Doherty II JJ, Kay DG, Posner BI, Bergeron JJM (1989b) Ligand mediated internalization,recycling, and down regulation of the epidermal growth factor receptor in vivo. J Cell Biol 109: 2741–2749Google Scholar
- Schaudies RP, Gorman RM, Savage CR, Poretz RD (1987) Proteolytic processing of epidermal growth factor within endosomes. Biochem Biophys Res Commun 143: 710715Google Scholar