Abstract
It is well known that molecules entering the cell do so via the early endosome. Within five minutes of internalization into animal cells, lipids, solutes and trans-membrane proteins (including receptors and their bound ligands), appear in early endosomes located predominately at the cell periphery (Griffiths and Gruenberg, 1991; Murphy, 1991). Molecules which return to the plasma membrane, in particular cell surface receptors, are rapidly recycled from the early endosome. In contrast, molecules destined for degradation are transported to the perinuclear late endosomes, and eventually to the lysosomes. The endocytic pathway is also connected, via vesicular traffic, to the biosynthetic pathway (Kornfeld and Mellman, 1989).
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References
Bailly, E., McCaffrey, M., Touchot, N., Zahraoui, A., Goud, B., and Bornens, M. (1991). Phosphorylation of two small GTP-binding proteins of the rab family by p34 cdc2. Nature 350, 715–718.
Baker, D., Wuestehube, L., Scheckman, R. and Segev, N. (1990). GTPbinding YPT1 protein and Ca2+ function independently in a cell-free protein transport reaction. PNAS USA 87, 355–359.
Balch, W. E. (1992). From G minor to G major. Current Biology 2, 157160
Burstein, E. S., Linko-Stentz, K., Lu, Z., and Macara, I. G. (1991). Regulation of the GTPase activity of the ras like protein p25-rab3a: evidence for a rab3a specific GAP. J. Biol. Chem. 266, 2689–2692.
Bomsel, M., Parton, R., Kuznetsov, S. A., Schroer, T. A., and Gruenberg, J. (1990). Microtubule and motor dependent fusion in vitro between apical and basolateral endocytic vesicles from MDCK cells. Cell 62, 719–731.
Bomsel, M., Prydz, K., Parton, R. G., Gruenberg, J., and Simons, K. (1989). Endocytosis in filter-grown Madin-Darby canine kidney cells. j. Cell Biol. 109, 3243–3258.
Bourne, H. (1988). Do GTPases direct membrane traffic in secretion? Cell 53, 669–671.
Bourne, H. R., Sanders, D. A., and McCormick, F. (1990). The GTPase superfamily: a conserved switch for diverse cell functions. Nature 348, 125–132.
Bucci, C., Parton, R. G., Mather, I. H. Stunnenberg, H. Simons, K., Hoflack, B., and Zerial, M. (in press). The small GTP-ase rab5 functions as a regulatory factor in the early endocytic pathway. Cell
Chavrier, P., Gorvel, J. P., Stelzer, E., Simons, K., Gruenberg, J., and Zerial, M. (1991). Hypervariable C-terminal domain of rab proteins acts as a targetting signal. Nature 353, 769–772.
Chavrier, P., Parton, R. G., Hauri, H. P., Simons, K., and Zerial, M. (1990). Localisation of low molecular weight GTP binding proteins to exocytic and endocytic compartments. Cell 62, 317–329.
Colombo, M. I., Mayorga, L. S., Casey, P. J., and Stahl, P. D. (1992). Evidence of a role for heterotrimeric GTP-binding proteins in endosome fusion. Science 255, 1695–1697.
D’Enfert, C., Wuestehube, L. J., Lila, T., and Schekman, R. (1991). Sec12pdependent membrane binding of the small GTP-binding protein Sar1 p promotes formation of transport vesicles from the ER. J. Cell Biol. 114, 663–670.
Diaz, R. Mayorga, L. S., Weidman, P. J., Rothman, J. E., and Stahl, P. (1989). Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport. Nature 339,398–400.
Fisher von Mollard, G., Mignerg, G., Baumert, M., Perin, M., Hanson, T., Burger, P., Jahn, R. and Sudhof, T. (1990). Rab3 is a small GTP-binding protein exclusively localized to synaptic vesicles. PNAS USA 87, 1988–1992.
Goda, Y., and Pfeffer, S. R. (1988). Selective recycling of the mannose 6phosphate/IGF-II receptor to the TGN in vitro. Cell 55, 309–320.
Gorvel, J. P., Chavrier, P., Zerial, M., and Gruenberg, J. (1991). Rab 5 controls early endosome fusion in vitro. Cell 64, 915–925.
Goud, B., Salminen, A., Walworth, N. C., and Novick, P. J. (1988). A GTPbinding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast. Cell 53, 753768.
Goud, B., Zahraoui, A., Tavitian, A. and Saraste, J. (1990) Small GTPbinding protein associated with Golgi cisternae. Nature 345, 553.
Goud, B., and McCaffrey, M. (1991). Small GTP-binding proteins and their role in transport. Curr. Opin. Cell Biol. 3, 626–633.
Griffiths, G., and Gruenberg, J. (1991). The arguments for pre-existing early and late endosomes. Trends Cell Biol. 1, 5–9.
Gruenberg, J., and Clague, M. (1992). Regulation of intracellular membrane transport. Curr. Op. Cell Biol. in press
Gruenberg, J., Griffiths, G., and Howell, K. E. (1989). Characterisation of the early endosome and putative endocytic carrier vesicles in vivo and with an ssay of vesicle fusion in vitro. J. Cell Biol. 108, 1301–1316.
Gruenberg, J., and Howell, K. E. (1989). Membrane traffic in endocytosis: insights from cell-free assays. Annu. Rev. Cell Biol. 5, 453–481.
Hopkins, C. R., Gibson, A., Shipman, M., and Miller, K. (1990). Movement of internalized ligand-receptor complexes along a continuous endosomal reticulum. Nature 346, 335–339.
Kabcenell, A. K., Goud, B., Northup, J. K., and Novick, P. J. (1990). Binding and hydrolysis of guanine nucleotides by sec4p, a yeast protein involved in the regulation of vesicular transport. J. Biol. Chem. 265, 9366–9372.
Kahn, R. A. (1991). Fluoride is not an activator of the smaller (20–25 kDa) GTP-binding proteins. J. Biol. Chem. 266, 15595–15597.
Kinsella, B. T., and Maltese, W. A. (1991). rab GTP-binding protein implicated in vesicular transport are isoprenylated in vitro at cysteines within a novel carboxy-terminal motif. J. Biol. Chem. 266, 8540–8544.
Kornfeld, S., and Mellman, I. (1989). The biogenesis of lysosomes. Annu. Rev. Cell Biol. 5, 483–526.
Lenhard, J. M., Kahn, R. A., and Stahl, P. D. (1992). Evidence for ADPribosylation factor (ARF) as a regulator of in vitro endosomeendosome fusion. 267, 13047–13052.
Marsh, H. M., Griffiths, G., Dean, G. E., Mellman, I. and Helenius, A. (1986). Three-dimensional structure of endosomes in BHK-21 cells. PNAS USA 83, 2899–2903.
Mayorga, L. S., Diaz, R., Colombo, M. I., and Stahl, P. D. (1989). GTPyS stimulation of endosome fusion suggests a role for a GTP-binding protein in the priming of vesicles before fusion. Cell Regulation 1, 113–124.
Mullock, B. M., Branch, W. J., vanSchaik, M., Gilbert, L. K., and Luzio, J. P. (1989). Reconstitution of an endosome-lysosome interaction in a cell-free system. J. Cell Biol. 108, 2093–2099.
Murphy, R. F. (1991). Maturation models for endosome and lysosome biogenesis. Trends Cell Biol. 1, 77–82.
Parton, R. G., Prydz, K., Bomsel, M., Simons, K., and Griffiths, G. (1989). Meeting of the apical and basolateral endocytic pathways of the Madin-Darby canine kidney cell in late endosomes. J. Cell Biol. 109, 3259–3272.
Pfeffer, S. (1992). GTP-binding proteins in intracellular transport. Trends Cell Biol. 2, 41–46.
Rexach, M. F., and Schekman, R. W. (1991). Distinct biochemical requirements for the budding, targeting and fusion of ER-derived transport vesicles. J. Cell Biol. 114, 219–229.
Rothman, J. E., and Orci, L. (1992). Molecular dissection of the secretory pathway. Nature 355, 409–415.
Salminen, A., and Novick, P. J. (1987). A ras-like protein is required for a post-Golgi event in yeast secretion. Cell 49, 527–538.
Segev, N., Mulholland, J., and Botstein, D. (1988). The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery. Cell 52, 915–924.
Serafini, T., Orci, L., Amherdt, M., Brunner, M., Kahn, R. A., and Rothman, J. E. (1991). ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP coated vesicles: a novel role for a GTP-binding protein. Cell 67, 239–253.
Thomas, L., Clarke, P., Pagano, M., and Gruenberg, J. (1992). Inhibition of membrane fusion in vitro via cyclin B but not cyclin A. J. Biol. Chem. 267, 6183–6187.
Tooze, J., and Hollinshead, M. (1991). Tubular early endosomal network in AtT20 and other cells. J. Cell Biol. 115, 635–654.
Tuomikoski, T., Felix, M., Doree, M., and Gruenberg, J. (1989). Inhibition of endocytic vesicle fusion in vitro by the cell-cycle control protein kinase cdc2. Nature 342, 942–945.
Valencia, A., Chardin, P., Wittinghofer, A., Sander, C. (1991). The ras protein family: evolutionary tree and role of conserved amino acids. Biochemistry 30, 4637–4648.
van der Sluijs, P., Hull, M., Zahraoui, A., Tavitian, A., Goud, B., and Mellman, I. (1991). The small GTP-binding protein rab4 is associated with early endosomes. Proc. Natl. Acad. Sci. 88, 6313–6317.
Wagner, P., Molenaar, C. M., Rauh, A. J., Brokel, R., Schmitt, H. D. and Gallwitz, D. (1997) Biochemical properties of the ras-related YPT1 protein in yeast: a mutational analysis. EMBO J. 6, 2373–2379.
Warren, G. (1989). Mitosis and membranes. Nature 342, 857–858.
Wessling-Resnick, M., and Braell, W. A. (1990). Characterization of the mechanism of endocytic vesicle fusion in vitro. J. Biol. Chem. 265, 16751–16759.
Woodman, P., Mundy, D. I., Cohen, P., and Warren, G. (1992). Cell-free fusion of endocytic vesicles is regulated by phosphorylation. J. Cell Biol. 116, 331–338.
Woodman, P., and Warren, G. (1991). Isolation of functional, coated endocytic vesicles. J. Cell Biol. 112, 1133–1141.
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© 1993 Springer-Verlag Berlin Heidelberg
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Steele-Mortimer, O., Clague, M.J., Thomas, L., Gorvel, JP., Gruenberg, J. (1993). Regulation of Early Endosome Fusion In Vitro. In: Morré, D.J., Howell, K.E., Bergeron, J.J.M. (eds) Molecular Mechanisms of Membrane Traffic. NATO ASI Series, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-02928-2_45
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DOI: https://doi.org/10.1007/978-3-662-02928-2_45
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