Targeting of the Polymeric Immunoglobulin Receptor in Transfected PC12 Cells
Synaptic vesicles are among the best characterized membranous organelles. A rat pheochromocytoma cell line, PC12, makes vesicles of the same size, density and membrane composition as authentic synaptic vesicles, isolated from rat brain (Clift-O’Grady et al., 1990). These organelles have been termed “synaptic vesicle-like vesicles” (SVLVs). In recent years PC12 cells have been used by several investigators as a model system for studying synaptic vesicle biogenesis and the targeting of synaptic vesicle-specific proteins. Evidence has accumulated from these studies that synaptic vesicles can be derived by endocytosis and that the precursor organelle in the nerve terminal is either the plasma membrane or an early endosomal compartment (Johnston et al., 1989; Clift-O’Grady et al., 1990; Regnier-Vigouroux et al., 1991).
KeywordsPC12 Cell Synaptic Vesicle Sodium Butyrate Transfected PC12 Cell Polymeric Immunoglobulin Receptor
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