Advertisement

Targeting of the Polymeric Immunoglobulin Receptor in Transfected PC12 Cells

  • F. Bonzelius
  • G. A. Hermani
  • M. H. Cardone
  • K. E. Mostov
  • R. B. Kelly
Conference paper
Part of the NATO ASI Series book series (volume 74)

Abstract

Synaptic vesicles are among the best characterized membranous organelles. A rat pheochromocytoma cell line, PC12, makes vesicles of the same size, density and membrane composition as authentic synaptic vesicles, isolated from rat brain (Clift-O’Grady et al., 1990). These organelles have been termed “synaptic vesicle-like vesicles” (SVLVs). In recent years PC12 cells have been used by several investigators as a model system for studying synaptic vesicle biogenesis and the targeting of synaptic vesicle-specific proteins. Evidence has accumulated from these studies that synaptic vesicles can be derived by endocytosis and that the precursor organelle in the nerve terminal is either the plasma membrane or an early endosomal compartment (Johnston et al., 1989; Clift-O’Grady et al., 1990; Regnier-Vigouroux et al., 1991).

Keywords

PC12 Cell Synaptic Vesicle Sodium Butyrate Transfected PC12 Cell Polymeric Immunoglobulin Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Apodaca G, Bomsel M, Arden J, Breitfeld PP, Tang K, Mostov KE (1991) The polymeric immunoglobulin receptor. J Clin Invest 87: 1877–1882PubMedCrossRefGoogle Scholar
  2. Casanova JE, Breitfeld PP, Ross SA, Mostov KE (1990) Phosphorylation of the polymeric immunoglobulin receptor required for its efficient transcytosis. Science 248: 742–745PubMedCrossRefGoogle Scholar
  3. Clift-O’Grady L, Linstedt AD, Lowe AW, Grote E, Kelly RB (1990) Biogenesis of synaptic vesicle-like structures in a pheochromocytoma cell line PC-12. J Cell Biol 110: 1693–1703Google Scholar
  4. Johnston PA, Cameron PL, Stukenbrok H, Jahn R, De Camilli P, Suedhof TC (1989) Synaptophysin is targeted to similar microvesicles in CHO and PC12 cells. EMBO J 8: 2863–2872PubMedGoogle Scholar
  5. Regnier-Vigouroux A, Tooze SA, Huttner WB (1991) Newly synthesized synaptophysin is transported to synaptic-like microvesicles via constitutive secretory vesicles and the plasmamembrane. EMBO J 10: 3589–3601PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • F. Bonzelius
    • 1
  • G. A. Hermani
    • 1
  • M. H. Cardone
    • 2
  • K. E. Mostov
    • 2
  • R. B. Kelly
    • 1
  1. 1.Department of Biochemistry and BiophysicsUniversity of CaliforniaSan FranciscoUSA
  2. 2.Department of AnatomyUniversity of CaliforniaSan FranciscoUSA

Personalised recommendations