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Lipid Transport from the Golgi to the Plasma Membrane of Epithelial Cells

  • G. van Meer
  • I. L. van Genderen
  • W. van ’t Hof
  • K. N. J. Burger
  • P. van der Bijl
Conference paper
Part of the NATO ASI Series book series (volume 74)

Abstract

The two plasma membrane domains of epithelial cells display unique protein and lipid compositions. The general feature of the lipid polarity appears to be an enrichment of glycosphingolipids on the apical, and of the phospholipid phosphatidylcholine, PC, on the basolateral surface. The difference is maintained by the tight junctions, the zone of cell-cell contacts that encircles the apex of each epithelial cell. This structure acts as a barrier to lipid diffusion in the outer leaflet of the plasma membrane (van Meer, 1989b). The differences in lipid composition must therefore reside in the outer leaflets of the apical and basolateral plasma membrane domain. While the compositional differences appear to be generated by sorting of newly synthesized lipids before they reach the cell surface, lipid sorting along the transcytotic route would also seem necessary to prevent lipid intermixing by that pathway.

Keywords

MDCK Cell Outer Leaflet Microtubule Depolymerization Plasma Membrane Domain Apical Cell Surface 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Brown DA, Rose JK (1992) Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell 68: 533–544PubMedCrossRefGoogle Scholar
  2. Coste H, Martel MB,. Got R (1986) Topology of glucosylceramide synthesis in Golgi membranes from porcine submaxillary glands. Biochim Biophys Acta 858: 6–12CrossRefGoogle Scholar
  3. Futerman AH, Pagano RE (1991) Determination of the intracellular sites and topology of glucosylceramide synthesis in rat liver. Biochem J 280: 295–302PubMedGoogle Scholar
  4. Jeckel D, Karrenbauer A, Burger KNJ, van Meer G, Wieland F (1992) Glucosylceramide is synthesized at the cytosolic surface of various Golgi subfractions. J Cell Biol 117: 259–267PubMedCrossRefGoogle Scholar
  5. Karrenbauer A, Jeckel D, Just W, Birk R, Schmidt RR, Rothman JE, Wieland FT (1990) The rate of bulk flow from the Golgi to the plasma membrane. Cell 63: 259–267PubMedCrossRefGoogle Scholar
  6. Kobayashi T, Pagano RE (1989) Lipid transport during mitosis. Alternative pathways for delivery of newly synthesized lipids to the cell surface. J Biol Chem 264: 5966–5973Google Scholar
  7. Kobayashi T, Pimplikar, SW, Parton, RG, Bhakdi, S, Simons, K (1992) Sphingolipid transport from the trans-Golgi network to the apical surface in permeabilized MDCK cells. FEBS Lett 300: 227–231PubMedCrossRefGoogle Scholar
  8. Lisanti MP, Rodriguez-Boulan E (1990) Glycophospholipid membrane anchoring provides clues to the mechanism of protein sorting in polarized epithelial cells. TIBS 15: 113–118PubMedGoogle Scholar
  9. Pagano RE (1990) Lipid traffic in eukaryotic cells: mechanisms for intracellular transport and organelle-specific enrichment of lipids. Curr Opin Cell Biol 2: 652–663PubMedCrossRefGoogle Scholar
  10. Pascher I (1976) Molecular arrangements in sphingolipids. Conformation and hydrogen bonding of ceramide and their implication on membrane stability and permeability. Biochim Biophys Acta 455: 433–451PubMedCrossRefGoogle Scholar
  11. Schwarzmann G, Sandhoff K (1990) Metabolism and intracellular transport of glycosphingolipids. Biochemistry 29: 10865–10871PubMedCrossRefGoogle Scholar
  12. Simons K, van Meer G (1988) Lipid sorting in epithelial cells. Biochemistry 27: 6197–6202PubMedCrossRefGoogle Scholar
  13. Sonnenberg A, van Balen P, Hengeveld T, Kolvenbag GJC, van Hoeven RP, Hilgers J (1986) Monoclonal antibodies detecting different epitopes on the Forssman glycolipid hapten. J Immunol 137: 1264–1269PubMedGoogle Scholar
  14. Trinchera M, Fabbri M, Ghidoni R (1991) Topography of glycosyltransferases involved in the initial glycosylations of gangliosides. J Biol Chem 266: 20907–20912PubMedGoogle Scholar
  15. van Genderen IL, van Meer G, Slot JW, Geuze HJ, Voorhout WF (1991) Subcellular localization of Forssman glycolipid in epithelial MDCK cells by immuno-electronmicroscopy after freeze-substitution. J Cell Biol 115: 1009–1019PubMedCrossRefGoogle Scholar
  16. van Meer G (1989a) Lipid traffic in animal cells. Annu Rev Cell Biol 5: 247–275PubMedCrossRefGoogle Scholar
  17. van Meer G (1989b) Polarity and polarized transport of membrane lipids in a cultured epithelium. Modern Cell Biology (Satir BH, ed), Functional Epithelial Cells in Culture. (Matlin KS, Valentich JD, eds), Alan R Liss Inc, New York 8: 43–69Google Scholar
  18. van Meer G, Simons K (1988) Lipid polarity and sorting in epithelial cells. J Cell Biochem 36: 51–58PubMedCrossRefGoogle Scholar
  19. van Meer G, Stelzer EHK, Wijnaendts-van-Resandt RW, Simons K (1987) Sorting of sphingolipids in epithelial ( Madin-Darby canine kidney) cells. J Cell Biol 105: 1623–1635Google Scholar
  20. van Meer G, van ‘t Hof (1992) Epithelial sphingolipid sorting is insensitive to reorganization of the Golgi by nocodazole, but is abolished by monensin and by brefeldin A. SubmittedGoogle Scholar
  21. van ’t Hof W, van Meer G (1990) Generation of lipid polarity in intestinal epithelial (Caco-2) cells: Sphingolipid synthesis in the Golgi complex and sorting before vesicular traffic to the plasma membrane. J Cell Biol 111: 977–986CrossRefGoogle Scholar
  22. Epithelial sphingolipid sorting allows for extensive variation of the fatty acyl chain and the sphingosine backbone. Biochem J 283: 913–917Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • G. van Meer
    • 1
  • I. L. van Genderen
    • 1
  • W. van ’t Hof
    • 1
  • K. N. J. Burger
    • 1
  • P. van der Bijl
    • 1
  1. 1.Department of Cell BiologyUtrecht University Medical SchoolUtrechtThe Netherlands

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