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Transport to the Cell Surface of Chlamydomonas: Mastigonemes as a Marker for the Flagellar Membrane

  • Mitchell Bernstein
  • Joel L. Rosenbaum
Conference paper
Part of the NATO ASI Series book series (volume 74)

Abstract

We have begun experiments to determine how proteins are targeted to different cell surface domains of the unicellular green alga, Chlamydomonas. Though contiguous, the flagellar membrane and plasma membrane of Chlamydomonas define physically and biochemically distinct compartments. The plasma membrane is surrounded by the cell wall, constructed from hydroxyproline rich glycoproteins, and a set of inducible enzymes, such as carbonic anhydrase and arylsulfatase,are specifically secreted into the periplasmic space. In contrast, the flagellar membrane is not surrounded by the cell wall and contains proteins not found on the plasma membrane, including agglutinins used for adhesion during mating, and a 350 kD protein required for gliding motility on solid substrates. (For reviews, see Harris, 1989, and Bloodgood, 1990.)

Keywords

Phosphotungstic Acid Periplasmic Space Unicellular Green Alga Trifluoromethanesulfonic Acid Distinct Compartment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Bloodgood,R A (1990) Gliding motility and flagellar glycoprotein dynamics in Chlamydomonas. In Ciliary and Flagellar Membranes. R A Bloodgood (ed.). Plenum Press, New York. 91–128CrossRefGoogle Scholar
  2. Harris,E H (1989) The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use. Academic Press, Inc., New York. 780 pp.Google Scholar
  3. Monk,B C, W S Adair, R A. Cohen, and U W Goodenough (1983) Topography of Chlamydomonas: Fine structure and polypeptide components of the gametic flagellar membrane surface and the cell wall. Planta 158: 517–533Google Scholar
  4. Witman,G, K Carlson, J Berliner, and J L Rosenbaum. (1972) Chlamydomonas flagella: I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes. J Cell Biol 54: 507–539Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Mitchell Bernstein
    • 1
  • Joel L. Rosenbaum
    • 1
  1. 1.Department of BiologyYale UniversityNew HavenUSA

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