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Effect of Brefeldin A Treatment on the Resident Golgi Protein, MG160

  • Patricia A. Johnston
  • Nicholas K. Gonatas
Conference paper
Part of the NATO ASI Series book series (volume 74)

Abstract

TheGolgi apparatus (GA) plays a crucial role in the post-translational processing and sorting of many cellular proteins. A small number of Golgi-specific proteins have been identified, including MG160 (Gonatas et al., 1989), an integral membrane sialoglycoprotein concentrated in the medial cisternae. Analysis of the biochemical properties of theseGolgi-specific proteins should provide insight into the molecular mechanisms which underlie GA function. Here we examine the effects of BFA treatment on the lifecycle of MG160 in order to determine if it undergoes unique carbohydrate processing or targeting within the GA. Our data indicate that BFA promotes the accumulation of a 150 Kd precursor to MG160 which is Endo-H resistant, but not sialylated. These results raise interesting questions about the transit of MG160 through the GA.

Keywords

PC12 Cell Sialic Acid Trans Golgi Network Golgi Protein Final Shift 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Doms RW, Russ G and YewdellJW (1989) Brefeldin A Redistributes Resident and Itinerent Golgi Proteins to the Endoplasmic Reticulum. J. CellBio1109: 61–72.Google Scholar
  2. Gonatas JO, Mezitis SGE, Stieber A, Fleischer and Gonatas NK (1989) MG160: A Novel Sialoglycoprotein of the Medial Cisternae of the Golgi Apparatus. JBC 264: 646–653.Google Scholar
  3. Lippincott-Schwartz J, Yuan LC, Bonificano, JS and Klausner RD (1989) Rapid Redistribution of Golgi Proteins into the ER in Cells Treated with Brefeldin A: Evidence for Membrane Cycling from Golgi to ER. Cell 56: 801–813.Google Scholar
  4. Roth J,Taatjes DJ, Lucocq JM, Weinstein J, Paulson JC (1985) Demonstration of an Extensive Trans-Tubular Network Continuous with the Golgi Apparatus Stack that may Function in Glycosylation. Cell 43: 287–295.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Patricia A. Johnston
    • 1
  • Nicholas K. Gonatas
    • 1
  1. 1.Dept. of Pathology and Laboratory MedicineU. of Pennsylvania School of MedicinePhiladelphiaUSA

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