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Transport of Microinjected Proteins into the Peroxisomes of Mammalian Cells

  • P. A. Walton
  • S. J. Gould
  • S. Subramani
Conference paper
Part of the NATO ASI Series book series (volume 74)

Abstract

Peroxisomes are single-membrane-bound organelles found in virtually all eukaryotic cells. Proteins destined for the peroxisomes are synthesized on free polysomes in the cytoplasm and are transported into the peroxisome post-translationally (Fujiki et al., 1984). Although at least two proteins, thiolase and acyl-CoA oxidase, undergo proteolytic processing after transport (Fujiki et al., 1985), most proteins are synthesized at their mature size. Import of proteins into the peroxisome is dependent upon, among other factors, the presence of a peroxisomal targeting signal on the newly synthesized protein. A C-terminal tripeptide peroxisomal targeting signal (PUS) with the sequence serine-lysine-leucine-COOH (or a conservative variant) was identified initially in firefly luciferase (Gould et al., 1987), and has subsequently been found in most peroxisomal proteins (reviewed by Subramani, 1992).

Keywords

Alcohol Oxidase Conservative Variant Normal Human Fibroblast Zellweger Syndrome Peroxisomal Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Fujiki, Y., R.A. Rachubinski, and P.B. Lazarow. 1984. Synthesis of a major integral membrane polypeptide of rat liver peroxisomes on free polysomes. Proc. Nat. Acad. Sci. USA. 81, 7127–7131.PubMedCrossRefGoogle Scholar
  2. Fujiki, Y., R.A. Rachubinski, R.M. Mortensen, and P.B. Lazarow. 1985. Synthesis of 3-ketoacyl-CoA thiolase of rat liver peroxisomes on free polysomes as a larger precursor. Induction of thiolase mRNA activity by clofibrate. Biochem. J. 226, 697–704.PubMedGoogle Scholar
  3. Gould, Si.,. G.-A. Keller, and S. Subramani 1987. Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase. J. Cell Biol. 105, 2923–2931.PubMedCrossRefGoogle Scholar
  4. Subramani, S (1992) Targeting of Proteins into the Peroxisomal Matrix. J. Membrane Biol. 125, 99–106.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • P. A. Walton
    • 1
  • S. J. Gould
    • 2
  • S. Subramani
    • 3
  1. 1.Dept. of AnatomyMcGill UniversityMontrealCanada
  2. 2.Kennedy InstituteJohns Hopkins Univ.BaltimoreUSA
  3. 3.Dept. of BiologyUniversity of CaliforniaLa JollaUSA

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