Hepatic Bile Formation: Intracellular Trafficking of Bile Salts

  • James M. Crawford
  • Stephen Barnes
  • Rebecca C. Stearns
  • Cynthia L. Hastings
  • Deborah C. J. Strahs
  • John J. Godleski
Conference paper
Part of the NATO ASI Series book series (volume 74)

Abstract

Bile formation facilitates the digestion and absorption of lipids from the gut, and provides a mechanism for the elimination of a wide variety of potentially toxic compounds from the body. The primary organic solutes in bile are amphiphilic bile salts and phospholipids, with the substantial flux of bile salts through the liver as part of the enterohepatic circulation providing the major driving force for bile formation. Although biliary phospholipid appears to be derived from intracellular pools, the interactions of bile salts with intracellular membranes and their transport through the hepatocyte remain poorly understood. We have examined the role of bile salt hydrophilicity in regulating bile salt interactions with microtubule-dependent membrane-based transport mechanisms, and have developed a novel methodological approach for the ultrastructural localization of bile salts within hepatocytes.

Keywords

Fluorine Paraformaldehyde 

References

  1. Crawford JM, Berken CA, Gollan JL (1988) Role of the hepatocyte micro-tubular system in the excretion of bile salts and biliary lipid: Implications for intracellular vesicular transport. J Lipid Research 29: 144–156Google Scholar
  2. Sweeny DJ, Daher G, Barnes S, Diasio RB (1990) Biological properties of the 2-fluoroß-alanine conjugates of cholic acid and chenodeoxycholic acid in the isolated perfused rat liver. Biochim Biophys Acta 1054: 21–25PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • James M. Crawford
    • 1
  • Stephen Barnes
    • 1
  • Rebecca C. Stearns
    • 1
  • Cynthia L. Hastings
    • 1
  • Deborah C. J. Strahs
    • 1
  • John J. Godleski
    • 1
  1. 1.Department of PathologyBrigham and Women’s HospitalBostonUSA

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