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Acyl Transfer Reactions Associated with Cis Golgi Apparatus

  • J. B. Lawrence
  • T. W. Keenan
  • P. Moreau
  • D. J. Morré
Conference paper
Part of the NATO ASI Series book series (volume 74)

Abstract

Recent work in our laboratory involving cell-free lipid transfer from transitional endoplasmic reticulum (ER) to Golgi apparatus has shown a latent phospholipase A activity localized in the cis Golgi of rat liver (Moreau 1991). Subsequently, phosphatidylethanolamine (PE) was shown to be an important component of ER to Golgi transport vesicles. Because PE has a small hydrophilic head group and a large hydrophobic tail, it has a conical shape which is very conducive to Hexagonal II phase structure formation. The possibility that we examined was that the lysophosphatidylcholine (lysoPC), formed by the phospholipase A activity, could interact with the PE to destabilize the Hexagonal II phase and form a bilayer. However, because levels of PE decrease through the Golgi stacks, the presence of lysoPC will then destabilize the bilayer structure. To retain a stabile bilayer structure, the lysoPC must be removed. This can be accomplished in two ways: 1) degradation by a Golgi lysophospholipid phospholipase or 2) reacylation by a Golgi acyl-CoA:lysoPC acyltransferase activity. The reacylation of Golgi apparatus lysoPC via the Lands pathway (Lands 1976) was investigated.

Keywords

Golgi Apparatus Bilayer Structure Acyltransferase Activity Golgi Stack Acyl Transfer Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. T. W. Keenan, Department of Biochemistry and Nutrition VPI Blacksburg, VA 24061Google Scholar
  2. P. Moreau, CNRS 1 rue Camille-Saint-Saens 33077 Bordeaux Cedex FranceGoogle Scholar
  3. Lands, W. E. M. and Crawford, C. G. (1976) Enzymes of Membrane Phospholipid Metabolism in Animals, In The Enzymes of Biological Membranes (Edited by Martouosi, A. ), Vol. 2, pp. 3–85 Plenum Press, New York.CrossRefGoogle Scholar
  4. Moreau, P. and Morré, D. J. (1991) Cell-Free Transfer of Membrane Lipids. Evidence For Lipid Processing, J. Biol. Chem. 266, 4329–4333Google Scholar
  5. Morré, D. J., Cheetham, R. D., Nyquist, S. E. and Ovtracht, L. (1972) A Simplified Procedure For Isolation of Golgi Apparatus From Rat Liver, Prep. Biochem. 2, 61–69Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • J. B. Lawrence
    • 1
  • T. W. Keenan
    • 1
  • P. Moreau
    • 1
  • D. J. Morré
    • 1
  1. 1.Department of Medicinal Chemistry 109 HANSPurdue UniversityWest LafayetteUSA

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