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Molecular Mechanisms of Membrane Traffic pp 65–66Cite as

A 38 kDa Protein Resident to CIS Golgi Apparatus Cisternae of Rat Liver is Recognized by an Antibody Directed Against α Subunits of Trimeric G-Proteins

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Part of the book series: NATO ASI Series ((ASIH,volume 74))

Abstract

Using a completely cell-free system from rat liver, we have demonstrated the formation of 50–70 nm ATP-dependent transfer vesicles and the transfer of both lipids and proteins to Golgi apparatus immobilized on nitrocellulose. The ATP-dependent transfer is specific for cis, and to a lesser degree, medial Golgi apparatus elements and not exhibited by trans Golgi apparatus elements. Therefore, evidence was sought for a cis-Golgi apparatus “docking” protein capable of specifically binding transition vesicles.

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Author information

Author notes

  1. A. O. Brightman

    Present address: Eli Lilly Company, Indianapolis, IN, USA

  2. W. Reutter

    Present address: Institut fur Molekulargiolggie und Biochemie, Freien Universitat Berlin, D-100, Berlin 33, Germany

Authors and Affiliations

  1. Department of Medicinal Chemistry & Pharmacognosy, Purdue University, West Lafayette, IN, USA

    A. O. Brightman, M. Paulik, J. B. Lawrence, T. Reust & D. J. Marré

  2. Institut fur Molekulargiolggie und Biochemie, Freien Universitat Berlin, D-100, Berlin 33, Germany

    C. C. Geilen

  3. Institut fur Pharmakologie, Freien Universitat Berlin, D-1000, Berlin 33, Germany

    K. Spicher

  4. Department of Foods and Nutrition, Purdue University, West Lafayette, IN, USA

    D. M. Morré

Authors
  1. A. O. Brightman
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  2. M. Paulik
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  3. J. B. Lawrence
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  4. T. Reust
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  5. C. C. Geilen
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  6. K. Spicher
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  7. W. Reutter
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  8. D. M. Morré
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  9. D. J. Marré
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Editor information

Editors and Affiliations

  1. Department of Medicinal Chemistry, HANS Life Science Research Building, Purdue University, West Lafayette, IN, USA

    D. James Morré

  2. C & S Biology, School of Medicine University of Colorado, Denver, CO, USA

    Kathryn E. Howell

  3. Department of Anatomy and Cell Biology, McGill University, H3A 2B2, Montreal, PQ, Canada

    John J. M. Bergeron

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© 1993 Springer-Verlag Berlin Heidelberg

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Cite this paper

Brightman, A.O. et al. (1993). A 38 kDa Protein Resident to CIS Golgi Apparatus Cisternae of Rat Liver is Recognized by an Antibody Directed Against α Subunits of Trimeric G-Proteins. In: Morré, D.J., Howell, K.E., Bergeron, J.J.M. (eds) Molecular Mechanisms of Membrane Traffic. NATO ASI Series, vol 74. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-02928-2_11

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  • DOI: https://doi.org/10.1007/978-3-662-02928-2_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-02930-5

  • Online ISBN: 978-3-662-02928-2

  • eBook Packages: Springer Book Archive

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