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A 38 kDa Protein Resident to CIS Golgi Apparatus Cisternae of Rat Liver is Recognized by an Antibody Directed Against α Subunits of Trimeric G-Proteins

  • A. O. Brightman
  • M. Paulik
  • J. B. Lawrence
  • T. Reust
  • C. C. Geilen
  • K. Spicher
  • W. Reutter
  • D. M. Morré
  • D. J. Marré
Conference paper
Part of the NATO ASI Series book series (volume 74)

Abstract

Using a completely cell-free system from rat liver, we have demonstrated the formation of 50–70 nm ATP-dependent transfer vesicles and the transfer of both lipids and proteins to Golgi apparatus immobilized on nitrocellulose. The ATP-dependent transfer is specific for cis, and to a lesser degree, medial Golgi apparatus elements and not exhibited by trans Golgi apparatus elements. Therefore, evidence was sought for a cis-Golgi apparatus “docking” protein capable of specifically binding transition vesicles.

Keywords

Golgi Apparatus Apparatus Membrane Transitional Endoplasmic Reticulum Transition Vesicle Trans Golgi Apparatus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Goud B, McCaffrey M (1991) Small GTP-binding proteins and their role in transport. Curr Opinion Cell Biol 3: 626–633PubMedCrossRefGoogle Scholar
  2. Burgoyne RD (1992) Trimeric G proteins in Golgi transport. Trends Biol Sci 17: 87–88CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • A. O. Brightman
    • 1
  • M. Paulik
    • 1
  • J. B. Lawrence
    • 1
  • T. Reust
    • 1
  • C. C. Geilen
    • 2
  • K. Spicher
    • 3
  • W. Reutter
    • 2
  • D. M. Morré
    • 4
  • D. J. Marré
    • 1
  1. 1.Department of Medicinal Chemistry & PharmacognosyPurdue UniversityWest LafayetteUSA
  2. 2.Institut fur Molekulargiolggie und BiochemieFreien Universitat BerlinBerlin 33Germany
  3. 3.Institut fur PharmakologieFreien Universitat BerlinBerlin 33Germany
  4. 4.Department of Foods and NutritionPurdue UniversityWest LafayetteUSA

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