Abstract
The importance of phospholipids in biological systems has become increasingly recognized in recent years. In T cells as in other cells, a majority of investigators have focused their research on the role of the phosphoinositide (Ptdlns) cycle as a system that generates the lipidic second messengers. Our studies on T lymphocytes have shown that other phospholipids and particularly phosphatidylserine (PtdSer) could play an essential role in T cell activation monitored by the measurement of interleukin-2 synthesis. The present paper takes stock of our current knowledge on the regulation of PtdSer synthesis during T cell activation and on its possible role in the regulation of interleukin-2 synthesis in Jurkat cells, a leukemic T cell line widely used as a model for the studies on lymphocyte activation.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Auberger, P., Mary, D., Breittmayer, J.P., Aussel, C., and Fehlmann, M. (1989) Chymotryptic type protease inhibitors blocked Ca++ response and IL-2 production in Jurkat T cells. J. Immunol. 142: 1253–1259.
Aussel, C., Pelassy C, and Rossi, B. (1990a) Breakdown of a phosphatidylcholine pool arising from the metabolic conversion of phosphatidylethanolamine as a novel source of diacylglycerol in activated T cells. J. Lipid Mediators 2: 103–116.
Aussel, C., Pelassy, C., Mary, D., Choquet, D., and Rossi, B. (1990b) Regulation of interleukin-2 production and phosphatidylserine synthesis in Jurkat T lymphocytes by K+ channel antagonists. Immunopharmacology 20: 97–103.
Aussel, C., Pelassy, C., Mary, D., Breittmayer, J.P., Cousin, J.L., and Rossi, B. (1991a) Calcium-dependent regulation of phosphatidylserine synthesis in control and activated Jurkat cells. J. Lipid Mediators 3: 267–282.
Aussel, C., Pelassy, C., and Breittmayer, J.P. (1991b) A diacylglycerol kinase is involved in the regulation of interleukin 2 synthesis in Jurkat T cells. Cellular Immunol.139: 333–341.
Bishop, W.R., Ganong, B.R., and Bell, R.M. (1986) Attenuation of sn-1,2-diacylglycerol second messengers by diacylglycerol kinase. Inhibition by diacylglycerol analogs in vitro and in human platelets. J. Biol. Chem. 261:6993–6998.
Bjerve, K.S. (1973a) The phospholipid substrates in the calcium stimulated incorporation of nitrogen bases into microsomal phospholipids. Biochim. Biophys. Acta 306: 396–403.
Bjerve, K.S. (1973b) The Ca2+ dependent biosynthesis of lecithin, phosphatidylethanolamine and phosphatidylserine in rat liver subcellular particles. Biochim. Biophys. Acta 296: 549–562.
Breittmayer, J.P., Aussel, C., Farahifar, D., Cousin, J.L., and Fehlmann, M. (1991) A phosphatidic acid sensitive intracellular pool of calcium is released by anti-CD3 in Jurkat T cells. Immunology 73: 134–139.
Buchanan, A.G. and Kanfer, J. (1980) The effect of various incubation temperature, particulate isolation and possible role of calmodulin on the activity of the base exchange enzyme of rat brain. J. Neurochem. 35: 814–822.
Chiller, J.M., DeFreitas, E., Chesnut, R., Grey, H., and Skidmore, B. (1982) Signal requirements for T lymphocyte activation. In Isolation Characterization and Utilization of T lymphocytes clones. C. Fathman and F. Fitch, Eds, Academic Press, N.Y. p.83–94.
Davis, L., and Lipsky, P.E. (1985) Signals involved in T-cell activation. 1) Phorbolesters enhance responsiveness but cannot replace intact accessory cells in the induction of mitogen-stimulated T cell proliferation. J.Immunol. 135: 2946–2952.
Dornand, J., Sekkat, C., Mani, J.C., and Gerber, M. (1987) Lipoxygenase inhibitors suppress IL-2 synthesis. Relationships with rise in Ca++ and the events dependent on protein kinase C activation. Immunol. Lett. 16:101–105.
Gaiti, A., De Medio, E., Brunetti, M., Amaducci, L., and Porcellati, G. (1974) Properties and function of the calcium dependent incorporation of choline, ethanolamine and serine into the phospholipids of isolated rat brain microsomes. J. Neurochem. 23: 1153–1159.
Gallin, E.K. (1986) Ionic channels in leukocytes. J.Leuko. Biol. 39: 241–250.
Gelfand, E.W. (1989) Transmembrane ion fluxes in T lymphocytes: Prerequisites or consequences of ligand-induced activation. In Advances in regulation of cell growth. Vol.1 Mond, J.J., Cambier, J.C. and Weiss, A. Eds Raven Press, N.Y. 6: 119–123.
Imboden, J.B., Weiss, A., and Stobo, J. (1985) The antigen receptor on a human T cell line initiates activation by increasing cytoplasmic free calcium. J. Immunol. 134: 633–638.
Jalink, K., Van Corven, E.J., and Moolenaar, W.H. (1990) Lysophosphatidic acid but not phosphatidic acid is a potent Ca2+ mobilizing stimulus for fibroblasts. Evidence for an extracellular site of action. J. Biol. Chem. 265, 12232–12237.
Kaibuchi, K., Takai, Y., and Nishizuka, Y. (1985) Protein kinase C and calcium ions in mitogenic response of macrophage depleted human peripheral lymphocytes. J. Biol. Chem. 260: 1366–1372.
Kanfer, J. (1972) Base exchange reactions of the phospholipids in rat brain particles. J. Lipid Res. 13, 468–476.
Kanfer, J., McCartney, D., and Hattori, H. (1988) Regulation of the choline, ethanolamine and serine base exchange activities of rat brain microsomes by phosphorylation and dephosphorylation. FEB S Lett. 240: 101–104.
Kanoh, H. Yamada, K. and Sakane, F. (1990) Diacylglycerolkinase. A key modulator of signal transduction? Trends Biol. Sci. 15: 47–50.
Larsson, E.A., Coutinho, A., and Martinez, C. (1980) A suggested mechanism for T lymphocyte activation. Implication on the acquisition of functional reactivities Immunol. Rev. 51: 61–64.
Mary, D., Aussel, C., Pelassy, C., and Fehlmann, M. (1988) IL-1 signaling for IL-2 production in T cells involves a rise in phosphatidylserine synthesis. J. Immunol. 141: 3078–3080.
Mary, D., Peyron, J.F., Auberger, P., Aussel, C., and Fehlmann, M. (1989) Modulation of T cell activation by differential regulation of the phosphorylation of two cytosolic proteins. Implication of both Ca2+ and cyclic AMP-dependent protein kinases. J. Biol. Chem. 264: 14498–14502.
Miura, T. and Kanfer, J. (1976) Studies on base-exchange reactions of phospholipids in rat brain. Heterogeneity of base exchange enzymes. Arch. Biochem. Biophys. 175: 654–660.
Moolenaar, W.H., Kruijer, W., Tilly, B.C., Verlaan, I., Bierman, A.J., and DeLaat, S.W. (1986) Growth factor like action of phosphatidic acid. Nature 323, 171–173.
Ng, J., Fredholm, B., Jondal, M., and Anderson, T. (1987) Characterization of PHA and anti-T3 induced transduction mechanisms in a human T cell leukemia line. Int. J. Immunopharmacol. 9: 17–21.
Nishizuka, Y. (1984) The role of protein kinase C in cell surface signal transduction and tumor promotion. Nature 308:693–696.
Palacios, R. (1982) Mechanism of T cell activation. Role and functional relationships of HLA-DR antigens and interleukins. J. Immunol. 63: 76–82.
Pelassy, C., Aussel, C., and Fehlmann, M. (1989a) Phospholipid metabolism and T cell activation. Receptor triggering is associated with the inhibition of phosphatidylserine synthesis. Cellular Signaling 1: 99–105.
Pelassy, C., Dallanegra, A., Aussel, C., and Fehlmann, M. (1989b) Inhibition of phosphatidylserine synthesis induced by triggering CD2 or the CD3-TCR complex in a human T cell line. Relationships with G proteins and receptors modulation. Mol. Immunol. 26: 1081–1086.
Pelassy, C., Mary, D., and Aussel, C. (1991a) Effects of the serine analogues isoserine and serinol on interleukin-2 synthesis and phospholipid metabolism in a human T cell line Jurkat. J. Lipid Mediators 3:79–89.
Pelassy, C., Breittmayer, J.P., Mary, D., and Aussel, C. (1991b) Inhibition of phosphatidylserine synthesis by phosphatidic acid in the Jurkat cell line: Role of calcium ions released from intracellular stores. J. Lipid Mediators 4: 199–210.
Pelassy, C., Mary, D. and Aussel, C. (1991c) Mechanism of phosphatidylserine induced inhibition of interleukin-2 synthesis in the human T cell line Jurkat. Immuno. Pharmacol. Toxicol. 12: 633–645.
Peyron, J.F., Aussel, C., Ferrua, B., Haring, H., and Fehlmann, M. (1989) Phosphorylation of two cytosolic proteins. An early event of T cell activation. Biochem. J. 258: 505–510.
Ponzin, D., Mancini, C., Toffano, G., Bruni, A., and Doria, G. (1989) Phosphatidylserine induced modulation of the immune response in mice. Effect of intravenous administration. Immunopharmacology 18: 167–173.
Rickard, J.E., and Sheterline, P. (1985) Evidence that phorbolesters interfere with stimulated Ca++ redistribution by activating Ca++ efflux in neutrophil lymphocytes. Biochem. J. 231: 623–628.
Roska, A.K. and Lipsky, P.E. (1985) Dissection of the functions of antigen-presenting cells in the induction of T cell activation. J.Immunol. 135: 2953–2959.
Sakane, F., Yamada, K., and Kanoh, H. (1989) Different effects of sphingosine, R59022 and anionic amphiphiles on two diacylglycerol kinase isozymes purified from porcine thymus. FEBS Lett. 255:409–413.
Weiss, A., Imboden, J., Shoback, D., and Stobo, J. (1984) Role of T3 surface molecules in human T cell activation. T3 dependent activation results in an increase in cytoplasmic free calcium. Proc. Natl. Acad. Sci. USA 81: 4169–4174.
Wiskocil, R., Weiss, A., Imboden, J., Kamin-Lewis, R., and Stobo, J. (1985) Activation of a human T cell line. A two stimulus requirement in the pretranslational events involved in the coordinate expression of interleukin-2 and interferon-g genes J.Immunol. 134: 633–639.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Aussel, C. (1993). Regulation of Phosphatidylserine Synthesis during T Cell Activation. In: Massarelli, R., Horrocks, L.A., Kanfer, J.N., Löffelholz, K. (eds) Phospholipids and Signal Transmission. Nato ASI Series, vol 70. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-02922-0_17
Download citation
DOI: https://doi.org/10.1007/978-3-662-02922-0_17
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-02924-4
Online ISBN: 978-3-662-02922-0
eBook Packages: Springer Book Archive