Membrane Protein Structure Determination by Deuterium-NMR Case Study: Retinal in Bacteriorhodopsin
To understand the function of a protein, one needs to establish the relationship between its three-dimensional structure, its dynamics and its environment. For many soluble proteins the primary, secondary and tertiary structures have been described in considerable detail, but a crucial gap in our knowledge is still the molecular structure of membrane proteins. These cannot usually be investigated by diffraction methods, because their amphipathic nature makes crystallization inherently difficult.
KeywordsNuclear Magnetic Resonance Quadrupole Splitting Purple Membrane Extracellular Side Thermal Isomerization
Unable to display preview. Download preview PDF.
- Stam, C. H. (1972) Acta Cryst. B28, 2936–2945Google Scholar