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Fluorescence-Activated Chromosome Sorting

  • J. A. Fantes
  • D. K. Green
Part of the Springer Laboratory book series (SLM)

Abstract

The DNA content of each human chromosome can be measured by flow cytometry, and the results of analyzing a large number of isolated metaphase chromosomes can be accumulated to form a flow karyotype. All the human chromosomes, except for 9–12, can be resolved [1], and small but useful quantities of individual chromosomes can be purified by flow sorting [2]. This material has been used to generate chromosome-specific recombinant DNA libraries which have been used by the scientific communitity as a source of markers linked to genes involved in genetic disease. Recently these libraries have also been used as a source of unique sequences for painting individual chromosomes in metaphase spreads by in situ hybridization [3]. Chromosome sorting has also been used for gene mapping, when cloned DNA probes are localized to a specific chromosome or subregion. This application can involve either filter hybridization [4] or a rapid technique based on the polymerase chain reaction (PCR) [5].

Keywords

Human Chromosome High Mitotic Index Flow Sorting Sheath Fluid Resuspend Pellet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • J. A. Fantes
  • D. K. Green

There are no affiliations available

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