Fluorescence-Activated Chromosome Sorting

  • J. A. Fantes
  • D. K. Green
Part of the Springer Laboratory book series (SLM)


The DNA content of each human chromosome can be measured by flow cytometry, and the results of analyzing a large number of isolated metaphase chromosomes can be accumulated to form a flow karyotype. All the human chromosomes, except for 9–12, can be resolved [1], and small but useful quantities of individual chromosomes can be purified by flow sorting [2]. This material has been used to generate chromosome-specific recombinant DNA libraries which have been used by the scientific communitity as a source of markers linked to genes involved in genetic disease. Recently these libraries have also been used as a source of unique sequences for painting individual chromosomes in metaphase spreads by in situ hybridization [3]. Chromosome sorting has also been used for gene mapping, when cloned DNA probes are localized to a specific chromosome or subregion. This application can involve either filter hybridization [4] or a rapid technique based on the polymerase chain reaction (PCR) [5].


Human Chromosome High Mitotic Index Flow Sorting Sheath Fluid Resuspend Pellet 
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© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • J. A. Fantes
  • D. K. Green

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