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Ligand Acidification by Nonadherent Cells

  • R. F. Murphy
Part of the Springer Laboratory book series (SLM)

Abstract

The goal of methods for ligand acidification by nonadherent cells is to obtain nearly continuous measurements of the pH to which a receptor-bound ligand is exposed after endocytosis. The protocols presented below are based on the method developed by Sipe and Murphy [1] for adherent (BALB/c3T3) cells and modified by Sipe et al. [2] for nonadherent (K562) cells. Reviews of acidification of endocytic compartments [3,4] and flow cytometric methods for analysis of endocytosis [5,6] may be consulted for additional background. The protocols below describe analysis of transferrin (Tf) acidification, but they may be easily modified for use with a different ligand.

Keywords

K562 Cell Nonadherent Cell Ligand Acidification Endocytic Compartment Flow Cytometric Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Sipe, D.M., Murphy, R.F. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 7119–7123PubMedCrossRefGoogle Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • R. F. Murphy

There are no affiliations available

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