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Biochemical Parameters of Cell Function

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Flow Cytometry and Cell Sorting

Part of the book series: Springer Laboratory ((SLM))

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Abstract

Cellular activation is characterized by early changes in functional parameters such as depolarization of the plasma membrane potential [1], increase in the cytosolic free calcium concentration [2], alkalinization of intracellular pH [3], increased mitochondrial charge [4], production and release of reactive oxidants [5], changes in cellular glutathione content [6], and activation and release of lysosomal proteases [7, 8]. These parameters are sensitive indicators of stimulus-coupled biochemical processes even before protein synthesis, gene transcription, or cellular proliferation is induced.

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Authors
  1. G. Rothe
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  2. G. Valet
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Editors and Affiliations

  1. Institut für Genetik, Universität Köln, Weyertal 121, 5000, Köln 41, Germany

    Andreas Radbruch

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© 1992 Springer-Verlag Berlin Heidelberg

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Rothe, G., Valet, G. (1992). Biochemical Parameters of Cell Function. In: Radbruch, A. (eds) Flow Cytometry and Cell Sorting. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-02785-1_12

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  • DOI: https://doi.org/10.1007/978-3-662-02785-1_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-02787-5

  • Online ISBN: 978-3-662-02785-1

  • eBook Packages: Springer Book Archive

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