Abstract
Cell cycle analysis of in vitro cell cultures is of relevance in basic and clinical research in various fields of immunology, cell biology and oncology. Historically cell proliferation has been studied using cell-counting techniques or radioactive thymidine labeling of S phase cells. With the advent of single cell analysis via flow cytometry interphase G1, S, and G2M cells were discernible. In addition, immunocytochemical techniques have been introduced selectively to label cycling G1/S/G2M phase cells (proliferation markers such as like proliferating cell nuclear antigen (PCNA) monoclonal antibodies, mAbs) or proliferating, BrdU-labeled S phase cells (BrdU mAbs)[1].
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© 1992 Springer-Verlag Berlin Heidelberg
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Kubbies, M. (1992). High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique. In: Radbruch, A. (eds) Flow Cytometry and Cell Sorting. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-02785-1_10
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DOI: https://doi.org/10.1007/978-3-662-02785-1_10
Publisher Name: Springer, Berlin, Heidelberg
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