Advertisement

Somaclonal Variation in Nicotiana sylvestris

  • D. Prat
  • R. De Paepe
  • X. Q. Li
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 11)

Abstract

Nicotiana sylvestris is a diploid species (2n = 24) from the Solanaceae family, native of the Andean foothills of northwestern Argentina, where it grows from 500 to 1600 m, generally in moist woods, but also on red silt and sandy banks. In 1899, Spegazzini and Comes described it as “a robust plant of the aspect of cultivated tobacco” (cited by Goodspeed 1954).

Keywords

Anther Culture Somaclonal Variation Protoplast Isolation Diploid Plant Protoplast Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aviv D, Galun E (1980) Restoration of fertility in cytoplasmic male sterile (CMS) Nicotiana sylvestris by fusion with X-irradiated N. tabacum protoplasts. Theor Appl Genet 58:121–128CrossRefGoogle Scholar
  2. Aviv D, Galun E (1986) Restoration of male fertile Nicotiana by fusion of protoplasts derived from two different cytoplasmic male-sterile cybrids. Plant Mol Biol 7:411–417CrossRefGoogle Scholar
  3. Aviv D, Galun E (1987) Chondriome analysis in sexual progenies of Nicotiana cybrids. Theor Appl Genet 73:821–826CrossRefGoogle Scholar
  4. Aviv D, Arzee-Gonen P, Bleichman S, Galun E (1984a) Novel alloplasmic Nicotiana plants by “donor-recipient” protoplast fusion: cybrids having N. tabacum or N. sylvestris nuclear genomes and either or both plastomes and chondriomes from alien species. Mol Gen Genet 196:244–253CrossRefGoogle Scholar
  5. Aviv D, Bleichman S, Arzee-Gonen P, Galun E (1984b) Intersectional cytoplasmic hybrids in Nicotiana: identification of plastomes and chondriomes in N. sylvestris + N. rustica cybrids having N. sylvestris nuclear genomes. Theor Appl Genet 67:499–504CrossRefGoogle Scholar
  6. Banks MS, Evans PK (1976) A comparison of the isolation and culture of nnesophyll protoplasts from several Nicotiana species and their hybrids. Plant Sci Lett 7:409–419CrossRefGoogle Scholar
  7. Berlyn MB (1983) Patterns of variability in DNA content and nuclear volume in regenerating cultures of Nicotiana tabacum. Can J Genet Cytol 25:354–360Google Scholar
  8. Bourgin JP, Nitsch JP (1967) Obtention de Nicotiana haploïdes à partir d’étamines cultivées in vitro. Ann Physiol Vég 9:377–382Google Scholar
  9. Bourgin JP, Missonier C, Chupeau Y (1976) Culture de protoplastes de mésophylle de Nicotiana sylvestris Spegazzini et Comes haploïde et diploïde. CR Acad Sci Paris Ser D 282:1853–1856Google Scholar
  10. Bourgin JP, Chupeau Y, Missonier C (1979) Plant regeneration from mesophyll protoplasts of several Nicotiana species. Physiol Plant 45:288–292CrossRefGoogle Scholar
  11. Brown JS, Wernsman EA, Schnell RJ (1983) Effect of a second cycle of anther culture on flue-cured lines of tobacco. Crop Sci 23:729–733CrossRefGoogle Scholar
  12. Csepló A, Nagy F, Maliga P (1983) Rescue of the cytoplasmic lincomycin resistance factor from Nicotiana sylvestris into Nicotiana plumbaginifolia by protoplast fusion. In: Potrykus I, Harms CT, Hinnen A, Hütter R, King PJ, Shillito RD (eds) Protoplasts 1983 — poster proceedings. Birkhäuser, Basel, pp 126–127Google Scholar
  13. D’Amato F (1985) Cytogenetics of plant cell and tissue cultures and their regenerates. CRC Crit Rev Plant Sci 3 :73–112CrossRefGoogle Scholar
  14. Deaton WR, Collins GB, Nielsen MT (1986) Vigor and variation expressed by anther-derived doubled haploids of burley tobacco (Nicotiana tabacum L.). II. Evaluation of first- and second-cycle doubled haploids. Euphytica 35:41–48CrossRefGoogle Scholar
  15. De Paepe R (1983) Etude génétique des plantes haploïdes-doublées (HD) obtenues par culture de pollen isolé chez Nicotiana sylvestris: aspects morphogénétiques, cellulaires et chromosomiques. Thése D d’Etat, Univ Paris XI-OrsayGoogle Scholar
  16. De Paepe R, Pernès J (1978) Exemples de variations à hérédité mendélienne induites au cours du développement des plantes. Physiol Vég 16:195–204Google Scholar
  17. De Paepe R, Nitsch C, Godard M, Pernès J (1977) Potential from haploid and possible use in agriculture. In: Barz W, Reinhard E (eds) Plant tissue culture and its bio-technological application. Springer, Berlin Heidelberg New York, pp 341–352Google Scholar
  18. De Paepe R, Bleton D, Gnangbe F (1981) Basis and extent of genetic variability among doubled haploid plants obtained by pollen culture in Nicotiana sylvestris. Theor Appl Genet 59:177–184CrossRefGoogle Scholar
  19. De Paepe R, Prat D, Huguet T (1982) Heritable nuclear DNA changes in doubled haploid plants obtained by pollen culture of Nicotiana sylvestris. Plant Sci Lett 28:11–28Google Scholar
  20. De Paepe R, Prat D, Knight J (1983) Effects of consecutive androgeneses on morphology and fertility in Nicotiana sylvestris. Can J Bot 61:2038–2046CrossRefGoogle Scholar
  21. Dhillon SJ, Wernsman EA, Miksche JP (1983) Evaluation of nuclear DNA content and heterochromatin changes in anther-derived dihaploids of tobacco (Nicotiana tabacum) cv. Coker 139. Can J Genet Cytol 25:169–173Google Scholar
  22. Dix PJ, Pearce RS (1981) Proline accumulation in NaCl resistant and sensitive cell lines of Nicotiana sylvestris. Z Pflanzenphysiol 102:243–248Google Scholar
  23. Dix PJ, Street HE (1974) Effect of p-fluorophenylalanine (PFP) on the growth of cell lines differing in ploidy and derived from Nicotiana sylvestris. Plant Sci Lett 3:283–288CrossRefGoogle Scholar
  24. Dix PJ, Street HE (1975) Sodium chloride-resistant cultured cell lines from Nicotiana sylvestris and Capsicum annuum. Plant Sci Lett 5:231–237CrossRefGoogle Scholar
  25. Dix PJ, Joo F, Maliga P (1977) A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin. Mol Gen Genet 157:285–290CrossRefGoogle Scholar
  26. Durand J (1979) High and reproducible plating efficiencies of protoplasts isolated from in vitro grown haploid Nicotiana sylvestris Spegaz. et Comes. Z Pflanzenphysiol 93:283–295Google Scholar
  27. Durand J (1984) Mutations de résistance en culture de cellules chez une plante supérieure, Nicotiana Google Scholar
  28. Durand J (1987) Isolation of antibiotic resistant variants in a higher plant, Nicotiana sylvestris. Plant Sci 51:113–118CrossRefGoogle Scholar
  29. Evans DA (1979) Chromosome stability of plants regenerated from mesophyll protoplasts of Nicotiana species. Z Pflanzenphysiol 95:459–463Google Scholar
  30. Evans DA, Bravo JE, Kut SA, Flick CE (1983) Genetic behavior of somatic hybrids in the genus Nicotiana: N. otophora + N. tabacum and N. sylvestris + N. tabacum. Theor Appl Genet 65:93–101CrossRefGoogle Scholar
  31. Wevans DA, Sharp R, Medina-Filho HP (1984) Somaclonal and gametoclonal variation. Am J Bot 71:759–774CrossRefGoogle Scholar
  32. Facciotti D, Pilet PE (1979) Plants and embryoids from haploid Nicotiana sylvestris protoplasts. Plant Sci Lett 15:1–6CrossRefGoogle Scholar
  33. Fluhr R, Aviv D, Edelman M, Galun E (1983) Cybrids containing mixed and sorted-out chloroplasts following interspecific somatic fusions in Nicotiana. Theor Appl Genet 65:289–294CrossRefGoogle Scholar
  34. Galun E, Arzee-Gonen P, Fluhr R, Edelman M, Aviv D (1982) Cytoplasmic hybridization in Nicotiana: mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitutions. Mol Gen Genet 186:50–56PubMedCrossRefGoogle Scholar
  35. Goodspeed TH (1954) The genus Nicotiana. Chronica botanica. Waltham, Mass, pp 1–536Google Scholar
  36. Goodspeed TH, Avery P (1939) Trisomic and other types in Nicotiana sylvestris. J Genet 38:381–467CrossRefGoogle Scholar
  37. Goodspeed TH, Clausen RE (1928) Interspecific hybridization in Nicotiana. VIII. The sylvestristomentosa-tabacum hybrid triangle and its bearing on the origin of tabacum. Univ Cal Publ Bot 11:245–256Google Scholar
  38. Hanson MR, Conde MF (1985) Functioning and variation of cytoplasmic genomes: lessons from cytoplasmic-nuclear interactions affecting male-sterility in plants. Int Rev Cytol 94:214–267Google Scholar
  39. Hayashi M, Nakajima T (1984) Genetic stability in regenerated plants derived from tabacco mesophyll protoplasts through three-step culture. Jpn J Breed 34:409–415Google Scholar
  40. Kostoff D (1934) A haploid plant of Nicotiana sylvestris. Nature (London) 23:949–950CrossRefGoogle Scholar
  41. Kostoff D (1938a) Studies on polyploid plants. XVIII. Cytogenetics studies on Nicotiana sylvestris × N. tomentosiformis hybrids and amphidiploids and their bearings on the problem of the origin of N. tabacum. C R Acad Sci USSR 18:459Google Scholar
  42. Kostoff D (1938b) The problem of haploidy — cytogenetic studies on Nicotiana haploids and their bearings to some other cytogenetic problems. Bibliogr Genet 13:1–147Google Scholar
  43. Kung S (1976) Tobacco fraction I protein — a unique genetic marker. Science 191:429–434PubMedCrossRefGoogle Scholar
  44. Larkin PJ, Scowcroft W R (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214CrossRefGoogle Scholar
  45. Li XQ (1987) Variabilité par culture in vitro: étude génétique à partir de culture de protoplastes chez Nicotiana svlvestris; description des plantes régénérées chez Medicago lupulina. Thèse D d’Etat, Univ Paris XI-OrsayGoogle Scholar
  46. Li XQ, Prat D, De Paepe R, Pernès J (1985) Variability induced in Nicotiana svlvestris by two successive cycles of protoplast culture. Genet Manipul Crops Newslett 1:81–84Google Scholar
  47. Li XQ, Chetrit P, Mathieu C, Vedel F, De Paepe R, Remy R, Ambard-Breteville F (1988) Regeneration of cytoplasmic male sterile protoclones of Nicotiana svlvestris with mitochondrial variation. Curr Genet 13:261–266CrossRefGoogle Scholar
  48. Lin M L, Staba S (1961) Peppermint and spearmint tissue culture. Callus formation in submerged culture. Lloydia 24:139–145Google Scholar
  49. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue culture. Physiol Plant 18:100–127CrossRefGoogle Scholar
  50. Maddox AD, Gonsalves F, Shields R (1983) Successful preservation of suspension cultures of three Nicotiana species at the temperature of liquid nitrogen. Plant Sci Lett 28:157–162Google Scholar
  51. Magnien E, Dalschaert X, Roumengous M, Devreux M (1980) Improvement of protoplast isolation and çulture technique from axenic plantlets of wild Nicotiana species. Acta Genet Sin 7:231–242Google Scholar
  52. Malepszy S, Grunewaldt J, Maluszynski M (1977) Über die Selektion von Mutanten in Zellkulturen aus haploider Nicotiana sylvestris Spegazz. et Comes. Z Pflanzenzücht 79:160–166Google Scholar
  53. Maliga P (1981) Streptomycin resistance is inherited as a recessive Mendelian trait in a Nicotiana svlvestris line. Theor Appl Genet 60:1–3CrossRefGoogle Scholar
  54. Maliga P, Lazar G, Joo F, Nagy AH, Menczel L (1977) Restoration of morphogenetic potential in Nicotiana by somatic hybridization. Mol Gen Genet 157:291–296CrossRefGoogle Scholar
  55. Maliga P, Kiss ZR, Dix PJ, Lazar G (1979) A streptomycin-resistant line of Nicotiana sylvestris unable to flower. Mol Gen Genet 172:13–15CrossRefGoogle Scholar
  56. McComb JA, McComb AJ (1977) The cytology of plantlets derived from cultured anthers of Nicotiana sylvestris. New Phytol 79:679–688CrossRefGoogle Scholar
  57. Medgyesy P, Menczel L, Maliga P (1980) The use of cytoplasmic streptomycin resistance — chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris, and isolation of their somatic hybrids. Mol Gen Genet 179:693–698CrossRefGoogle Scholar
  58. Misoo S, Takeuchi H, Fukuhara K, Matsubayashi M (1984) Studies on the mechanisms of pollen embryogenesis. V. Effects of genome constitution, ploidy level and cytoplasm on anther culture in tobacco and its ancestral species. Sci Rep Fac Agric Kobe Univ 16:35Google Scholar
  59. Murakishi HH, Carlson PS (1982) In vitro selection of Nicotiana sylvestris variant with limited resistance to TMV. Plant Cell Rep 1:94–97CrossRefGoogle Scholar
  60. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  61. Nagata T, Takebe I (1971) Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta 99:12–20CrossRefGoogle Scholar
  62. Nagy JI, Maliga P (1976) Callus induction and plant regeneration from mesophyll protoplasts of Nicotiana sylvestris. Z Pflanzenphysio 178:453–455Google Scholar
  63. Nagy F, Lazar G, Menczel L, Maliga P (1983) A heteroplasmic state induced by protoplast fusion is a necessary condition for detecting rearrangements in Nicotiana mitochondrial DNA. Theor Appl Genet 66:203–207CrossRefGoogle Scholar
  64. Negrutiu I, Mousseau J (1980) Protoplast culture from in vitro grown plants of Nicotiana sylvestris Spegg. and Comes. Z Pflanzenphysiol 100:373–376Google Scholar
  65. Negrutiu I, Mousseau J (1981) Culture sur grande échelle de protoplastes de Nicotiana sylvestris Spegazzini et Comes à partir de plantes cultivées in vitro. Z Pflanzenphysiol 103:367–372Google Scholar
  66. Negrutiu I, Muller JF (1981) Culture conditions of protoplast-derived cells of Nicotiana sylvestris for mutant selection. Plant Cell Rep 1:14–17CrossRefGoogle Scholar
  67. Negrutiu I, Jacobs M, Caboche M (1984a) Advances in somatic cell genetics of higher plants — the protoplast approach in basic studies on mutagenesis and isolation of biochemical mutants. Theor Appl Genet 67:289–304CrossRefGoogle Scholar
  68. Negrutiu I, Cattoir-Reynearts A, Verbruggen I, Jacobs M (1984b) Lysine overproducer mutants with an altered dihydrodipicolinate synthase from protoplast culture of Nicotiana sylvestris (Spegazzini and Comes). Theor Appl Genet 68:11–20CrossRefGoogle Scholar
  69. Niizeki M, Saito K, Kita F (1982) Studies on plant cell and tissue culture. XII. Anther culture of various polyploid and aneuploid plants in Nicotiana sylvestris. Bull Fac Agric Hirosaki Univ 37:20–28Google Scholar
  70. Niizeki M, Hayashi H, Saito K (1984) Production of disomic haploids by anther culture of a series of trisomic plants in Nicotiana sylvestris. Jpn J Breed 34:1–8Google Scholar
  71. Nitsch C (1974) Pollen culture — a new technique for mass production of haploids and homozygous plants. In: Kasha KJ (ed) Haploids in higher plants — advances and potential. Univ Press, Guelph, pp 123–135Google Scholar
  72. Nitsch C, Norreel B (1973) Effet d’un choc thermique sur le pouvoir embryogène du pollen de Datura innoxia cultivé dans l’anthère et isolé de l’anthère. CR Acad Sci Paris Ser D 276:303–306Google Scholar
  73. Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19:389–401Google Scholar
  74. Picard E (1984) Contribution à l’étude de l’hérédité et de l’utilisation en sélection de l’haplodiploïdisation par androgenèse chez une céréale autogame: Triticum aestivum L. Thèse D d’Etat, Univ Paris XI-OrsayGoogle Scholar
  75. Prat D (1982) Etude de la variabilité génétique induite au cours de cycles successifs d’androgenèse, et par culture de protoplastes chez Nicotiana sylvestris Spegaz. et Comes. Thèse d’ème Cycle, Univ Paris XI-OrsayGoogle Scholar
  76. Prat D (1983) Genetic variability induced in Nicotiana sylvestris by protoplast culture. Theor Appl Genet 64:223–230CrossRefGoogle Scholar
  77. Prat D, Gnangbe F, De Paepe R (1983) Alterations of leaf characteristics and peroxidase activity in diploid androgenetic lines of Nicotiana sylvestris Spegaz. and Comes. Plant Sci Lett 31:19–34CrossRefGoogle Scholar
  78. Rashid A, Street HE (1974) Segmentation in microspores of Nicotiana sylvestris and Nicotiana tabacum with lead to embryoid formation in anther culture. Protoplasma 80:323–334CrossRefGoogle Scholar
  79. Restivo FM, Tassi F (1986) Effect of 5-fluorouracil on growth and morphogenesis of tissue culture of Nicotiana sylvestris. Plant Cell Physiol 27:785–790Google Scholar
  80. Scowcroft WR (1984) Genetic variability in tissue culture: impact on germplasm conservation and utilization. Int Bd Plant Genet Resourc, Rome, pp 1–41Google Scholar
  81. Steinbrück G (1986) Molecular reorganisation during nuclear differentiation in ciliates. In: Hennig W (ed) Germ line — soma differentiation. Springer, Berlin Heidelberg New York Tokyo, pp 105–174Google Scholar
  82. Tomes DT, Collins G B (1976) Factors affecting haploid plant production from in vitro anther cultures of Nicotiana species. Crop Sci 16:837–840CrossRefGoogle Scholar
  83. Tsala G, Brettell R, Zryd JP (1983) Attempt to isolate auxotrophic mutants, using protoplasts of Nicotiana sylvestris. In: Potrykus I, Harms CT, Hinnen A, Hütter R, King PS, Shillito RD (eds) Protoplasts 1983 — poster proceedings. Birkhäuser, Basel, pp 160–161Google Scholar
  84. Van Slogteren GMS, Planque K, Lekkerkerk J (1980) Evaluation of parameters affecting the initiation of division of protoplasts of haploid and diploid Nicotiana svlvestris and Nicotiana tabacum. Plant Sci Lett 20:35–45CrossRefGoogle Scholar
  85. White DWR, Vasil IK (1979) Use of amino acid analogue-resistant cell lines for selection of Nicotiana sylvestris somatic cell hybrids. Theor Appl Genet 55:107–112CrossRefGoogle Scholar
  86. Zelcer A, Aviv D, Galun E (1978) Interspecific transfer of cytoplasmic male sterility by fusion between protoplasts of normal Nicotiana svlvestris and X-ray irradiated protoplasts of male-sterile N. tabacum. Z Pflanzenphysiol 90:397–407Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • D. Prat
    • 1
  • R. De Paepe
    • 2
  • X. Q. Li
    • 3
  1. 1.Laboratoire de Génétique et Physiologie du Développement des PlantesCNRSGif-sur-YvetteFrance
  2. 2.Laboratoire de Génétique Moléculaire des Plantes, UA 115Université Paris-SudOrsay CedexFrance
  3. 3.University of BeijingBeijingChina

Personalised recommendations