Somaclonal Variation in Carnations

  • B. Leshem
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 11)


The carnation (Dianthus caryophyllus L.) is a member of the Caryophyllaceae family. It is a semi-hardy perennial with branching stems, bearing linear, glaucous leaves in opposite and decussate pairs. Each stem forms a terminal flower and the inflorescence is a loose cyme. The flowering shoot can be treated for marketing in one of two forms: either the flower buds formed on short lateral shoots arising from the axils of the upper leaves are removed to leave one large, terminal flower on a long leafy stem (“standard” type), or the terminal flower bud is removed at an early stage to encourage more even development of the lateral flowers, which then produce a multiple-flowered stem (“spray” or “miniature” types). Special cultivars have been selected for spray production.


Shoot Apex Somaclonal Variation Carnation Plant Flower Stalk Periclinal Chimera 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Broertjes C, van Harten AM (1978) Application of mutation breeding methods in the improvement of vegetatively propagated crops. Elsevier, Amsterdam, pp 156–162Google Scholar
  2. Bunt AC, Cockshull KE (1985) Dianthus caryophyllus. In: Halevy AH (ed) Handbook of flowering, vol 2. CRC, Boca Raton. Fl, pp 433–440Google Scholar
  3. Bush SR, Earle E, Langhans RW (1976) Plantlets from petal segments, petal epidermis and shoot tips of the periclinal chimera. Chrysanthemum morifolium Indianapolis. Am J Bot 63:729–737CrossRefGoogle Scholar
  4. Debergh P. Harbaoui Y, Lemeur R (1981) Mass propagation of globe artichoke (Cynara scolymus). Evaluation of different hypotheses to overcome vitrification with special references to water potential. Physiol Plant 53:181–187CrossRefGoogle Scholar
  5. Dommergues P, Gillot J (1973) Obtention de clones génétiquement homogènes dans toutes leurs couches ontogéniques à partir d’une chimère d’oeillet américain. Ann Amelior Plantes 23:88–93Google Scholar
  6. Earle E. Langhans RW (1975) Carnation propagation from shoot tips cultured in liquid medium. Hort Sci 10:608–610Google Scholar
  7. Engvild KC (1972) Callus and cell suspension cultures of carnation. Physiol Plant 26:62–66CrossRefGoogle Scholar
  8. Erler R. Siegmund I (1986). Yearbook of the international horticultural statistics. Int Assoc Hortic Prod. Inst Gartenbauökon Univ Hannover 34:45Google Scholar
  9. Evans DA, Sharp W R. Medina-Filho HP (1984) Somaclonal and gametoclonal variation. Am J Bot 71:759–774CrossRefGoogle Scholar
  10. Farestveit B(1969) Flower colour chimeras in glasshouse carnations, Dianthus caryophyllus L. Yearb R Vet Agric Coll Copenhagen 1969:19–33Google Scholar
  11. George EF, Sherrington PD (1984) Plant propagation by tissue culture. Exegetics, Eversley Basingstoke, England, pp 74–83Google Scholar
  12. Gimelli F, Ginatta G, Venturo R, Positano S, Buiatti M (1984) Plantlet regeneration from petals and floral induction in vitro in the Mediterranean carnation (Dianthus caryophyllus L.). Riv Ortoflorofrutt It 68:107–121Google Scholar
  13. Hackett WP, Anderson JM (1967) Aseptic multiplication and maintenance of differential carnation shoot tissue derived from shoot apices. Proc Am Soc Hortic Sci 90:365–369Google Scholar
  14. Hartmann HT, Kester DE (1983) Plant propagation. Prentice Hall, New Jersey, pp 527–532Google Scholar
  15. Hoogendoorn C (1987) International developments in production and consumption of carnations. In: Book of abstracts. 3rd Int Symp Carnation, Noordwijkerhout, Neth, p 19Google Scholar
  16. Jelaska S, Sutina R (1977) Maintained culture of multiple plantlets from carnation shoot tips. Acta Hortic 78:333–337Google Scholar
  17. Johnson RT (1980) Gamma irradiation and in vitro — induced separation of chimeral genotypes in carnation. HortSci 15:605–606Google Scholar
  18. Kakehi M (1970) Studies on the tissue culture of carnation. I. Relationships between the growth and histodifferentiation of callus and each tissue of aseptic culture. Bull Hiroshima Agric College 4:40–49Google Scholar
  19. Kakehi M (1972) Studies on the tissue culture of carnation. II. Cytological studies on cultured cells. J Jpn Soc Hortic Sci 41:72–75CrossRefGoogle Scholar
  20. Kakehi M (1975) Studies on the tissue culture ofcarnation. IV. Callus suspension culture. Bull Hiroshima Agric College 5 :133–137Google Scholar
  21. Kakehi M (1979) Studies on the tissue culture of carnation. V. Induction of redifferentiated plants from the petal tissue. Bull Hiroshima Agric College 6:159–166Google Scholar
  22. Larkin PJ, Scowcroft WR (1981) Sornaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214CrossRefGoogle Scholar
  23. Leshem B (1983a) Growth of carnation meristems in vitro: Anatomical structure of abnormal plantlets and the effect of agar concentration in the medium on their formation. Ann Bot (London) 52:413–415Google Scholar
  24. Leshem B (1983b) The carnation succulent plantlet — a stable teratological growth. Ann Bot (London) 52:873–876Google Scholar
  25. Leshem B (1985) Carnation plants from vitrified plantlets, callus and petals — a possible source of somaclonal variation. Hassadeh 66:534–536 (Hebrew with English summary)Google Scholar
  26. Leshem B (1986) Carnation plantlets from vitrified plants as a source of somaclonal variation. HortSci 21:320–321Google Scholar
  27. Leshem B, Sachs T (1985) Vitrified Dianthus — teratomata in vitro due to growth factor imbalance. Ann Bot (London) 56:613–617Google Scholar
  28. Mehlquist GAL, Geissman TA (1947) Inheritance in the carnation (Dianthus caryophyllus). III. Inheritance of flower color. Ann Missouri Bot Garden 34:39–74CrossRefGoogle Scholar
  29. Mii M, Cheng SM (1982) Callus and root formation from mesophyll protoplasts of carnation. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo. pp 585–586Google Scholar
  30. Pereau-Leroy P(1974) Genetic interaction between the tissues of carnation petals as periclinal chimeras. Radiat Bot 14:109–116CrossRefGoogle Scholar
  31. Petru R. Landa Z (1974) Organogenesis in isolated carnation plant callus tissue cultivated in vitro. Biol Plant 16:450–453CrossRefGoogle Scholar
  32. Phillips DJ, Matthews GJ (1964) Growth and development of carnation shoot tips in vitro. Bot Gaz 125 :7–12CrossRefGoogle Scholar
  33. Richter A, Singleton WR (1955) The effect of chronic gamma radiation on the production of somatic mutations in carnations. Proc Natl Acad Sci USA 41:295–300PubMedCrossRefGoogle Scholar
  34. Roest S, Bokelmann GS (1981) Vegetative propagation of carnation in vitro through multiple shoot development. Sci Hortic 14:357–366CrossRefGoogle Scholar
  35. Sagawa Y, Mehlquist GAL (1957) The mechanism responsible for some X-ray induced changes in flower color of the carnation, Dianthus caryophyllus. Am J Bot 44:397–403CrossRefGoogle Scholar
  36. Shabde M, Murashige T(1977) Hormonal requirements of excised Dianthus caryophyllus L. shoot apical meristem in vitro. Am J Bot 64:443–448CrossRefGoogle Scholar
  37. Sparnaaij LD. Demmink JF (1983) Carnation of the future. Acta Hortic 141:17–23Google Scholar
  38. Sparnaay LD, Demmink JF, Garretsen F (1974) Clonal selection in carnation. In: Eucarpia Meet Ornamentals. Freius Inst Hortic Plant Breed, Neth, pp 39–50Google Scholar
  39. Stone OM (1963) Factors affecting the growth of carnation plants from shoot apices. Ann Appl Biol 52:199–209CrossRefGoogle Scholar
  40. Stone OM (1968) The elimination of four viruses from carnation and sweet william by meristem tip culture. Ann Appl Biol 62:119–122CrossRefGoogle Scholar
  41. Sutter E, Langhans EW (1981) Abnormalities in chrysanthemum regenerated from long term cultures. Ann Bot (London) 48:559–568Google Scholar
  42. Tilney-Bassett RAE (1986) Plant chimeras. Arnold, London, pp 122–136Google Scholar
  43. Tran Thanh Van M (1973) Direct flower neoformation from superficial tissue of small explants of Nicotiana tabacum. Planta 115:87–92CrossRefGoogle Scholar
  44. Vieitez AM, Ballester A, San Jose MC, Vieitez E (1985) Anatomical and chemical studies of vitrified shoots of chestnut regenerated in vitro. Physiol Plant 65:177–184CrossRefGoogle Scholar
  45. Villalobos V(1981) Floral differentiation in carnation (Dianthus caryophyllus L.) from anthers cultured in vitro. Phyton 41:71–75Google Scholar
  46. Werker E. Leshem B (1987) Structural chariges during vitrification of carnation plantlets. Ann Bot (London) 59:377–385Google Scholar
  47. Weryszko E. Hempel M (1979) Studies on in vitro multiplication of carnations. II. The histological analysis of mutliplantlets formation. Acta Hortic 91:323–331Google Scholar
  48. Ziv M, Meir G, Halevy AH (1983) Factors influencing the production of hardened glaucous carnation plantlets in vitro. Plant Cell Tissue Org Cult 2:55–65CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • B. Leshem
    • 1
  1. 1.Department of Plant Genetics and BreedingARO. The Volcani CenterBet DaganIsrael

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