Somaclonal Variation in Rice

  • Z.-X. Sun
  • K.-L. Zheng
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 11)


Rice is the staple food of more than half of the world’s population, and its present cultivation spans from latitude 53° N to 40° S (Lu and Chang 1980), from -23 to +7000 feet above sea level. It is cultivated under various conditions, including upland, deep water, and soils with pH 3.5 to 9.5 (Khush 1984). There are 22 species in the genus Oryza, most of which are diploid (2n = 24), and seven species are tetraploid (4n = 48) (Chang 1985). O. sativa L. and O. glaberrima Steud are the two cultivated species. O. sativa is usually divided into three eco-species: indica, japonica (sinica), and javanica.


Somatic Embryogenesis Rice Plant Plant Regeneration Anther Culture Somaclonal Variation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Abdullah R, Cocking EC, Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology 4:1087–1090CrossRefGoogle Scholar
  2. Abe T, Futsuhara Y (1985) Efficient plant regeneration by somatic embryogenesis from root calius tissues of rice (Orvza sativa L.). J Plant Physiol 121:111–118CrossRefGoogle Scholar
  3. Abe T, Futsuhara Y (1986a) Genotypic variability for callus formation and plant regeneration in rice (Oryza sativa L.). Theor Appl Genet 72:3–10CrossRefGoogle Scholar
  4. Abe T, Futsuhara Y (1986b) Plant regeneration from suspension cultures of rice (Oryza sativa L.) Jpn J Breed 36:1–6Google Scholar
  5. Abrigo WM, Novero AU, Coronel VP, Cabuslay GS, Blanco LC, Parao FT, Yoshida S (1985) Somatic cell culture at IRRI. In: Biotechnology in international agricultural research. IRRI, Manila, pp 144–158Google Scholar
  6. Ameniya A, Akemine H, Toriyama K (1956) Cultural conditions and growth of immature embryo in rice plant studies on the embryo culture in rice plant. 1. Bull Natl Inst Agric Sci D 6:1–40Google Scholar
  7. Anon (1966) The characteristics and cultivation technique of Ai-Jiao-Nan-Te, Oryza sativa L. subsp. hsien. Acta Agron Sin 5:18–22Google Scholar
  8. Anon (1978a) A study of the animal and plant remains unearthed at Ho-Mu-Tu. Acta Arch Olog Sin (1):95–107Google Scholar
  9. Anon (1978b) U.K. investigates virus insecticides. Nature (London) 276:548–549Google Scholar
  10. Anon (1986) DNAP develops patented protected “hot” tomato. Agric Genet Rep 5(5):4Google Scholar
  11. Arreghini E, Baratta G, Barbuze F, Biasini MG, Gavazzi G, Sale F, Todesco G, Tonelli C, Vecchio F (1986) Chemically induced mutagenesis versus somaclonal variation in tomato. In: Abstr 6th Int Congr Plant tissue and cell culture, Aug 3–8, 1986, Univ Min. Minneapolis, p 312Google Scholar
  12. Asselin de Beauville M (1980) Obtention d’haploides in vitro à partir d’ovaires non fécondés de riz, Oryza sativa L. C R Acad Sci Paris D 296:489–492Google Scholar
  13. Baba A, Hasezawa, Syono K (1986) Cultivation of rice protoplast and their transformation mediated by Agrobacterium spheroplasts. Plant Cell Physiol 27:463–471Google Scholar
  14. Bajaj YPS (1980) Induction of androgenesis in rice anthers frozen at -196 ° C. Cereal Res Commun 8:365–369Google Scholar
  15. Bajaj YPS (1981) Growth and morphogenesis in frozen (-196 ° C) endosperm and embryos of rice. Curr Sci 50:947–948Google Scholar
  16. Bajaj YPS (1983) Survival of somatic hybrid protoplasts of wheat x pea and rice x pea subjected to -196°C. Indian J Exp Biol 21:120–122Google Scholar
  17. Bajaj YPS (1984) The regeneration of plants from frozen pollen embryos and zygotic embryos of wheat and rice. Theor Appl Genet 67:525–528CrossRefGoogle Scholar
  18. Bajaj YPS (ed) (1986a) Biotechnology in agriculture and forestry, vol 2. Crops I. Springer, Berlin Heidelberg New York TokyoGoogle Scholar
  19. Bajaj YPS (1986b) In vitro preservation ofgenetic resources. In: Int Symp Nuclear techniques and in vitro culture for plant improvement. IAEA, Vienna, pp 43–57Google Scholar
  20. Bajaj YPS, Bidani M (1980) Differentiation of genetically variable plants from embryo-derived callus of rice. Phytomorphology 30:290–294Google Scholar
  21. Bajaj YPS, Bidani M (1986) In vitro induction of genetic variability in rice (Oryza sativa L.). Int Symp New genetical approaches to crop improvement. PIDC, Karachi, Pakistan, pp 63–74Google Scholar
  22. Bajaj YPS, Saini SS, Bidani M (1980) Production of triploid plants from the immature and mature endosperm cultures of rice. Theor Appl Genet 58:17–18Google Scholar
  23. Beachell HM, Khush GS, Julino BO (1972) Breeding for high protein content in rice. In: Rice breeding. IRRI, Manila, pp 419–428Google Scholar
  24. Bhattacharya P, Sen SK (1980) Potentiality of leaf sheat cells for regeneration of rice (Oryza sativa L.) plants. Theor Appl Genet 58:87–90Google Scholar
  25. Bounharmont J (1961) Embryo culture of rice on sterile medium. Euphytica 10:283–293CrossRefGoogle Scholar
  26. Bright S, Ooms G, Karp A, Foulger D, Fish N, Jones M, Evans N (1984) Somaclonal variation and genetic manipulation of potato. In: Abstr Int Symp Genetic manipulation in crops, Oct 22–26, 1984, BeijingGoogle Scholar
  27. Carlberg I, Soderhall K, Glimelius K, Eriksson T (1984) Protease activities in non-embryogenic and embryogenic carrot cell strains during callus growth and embryo formation. Physiol Plant 62:458–464CrossRefGoogle Scholar
  28. Carlson PS (1973) Methionine sulfoximine-resistant mutants of tobacco. Science 180:1366–1368PubMedCrossRefGoogle Scholar
  29. Cella R, Colombo R, Calli MG, Nielson E, Rollo F, Sala F (1982) Freeze-preservation of rice cells: a physiological study of freeze-thawed cells. Physiol Plant 55:279–284CrossRefGoogle Scholar
  30. Chaleff RS (1975) Higher plant cells experimental organisms. In: Markham R, Davies DR, Hopwood DA, Horne RW (eds) Modification of the information content of plant cells. Elsevier/North Holland Biomedical Press, Amsterdam New York, pp 197–214Google Scholar
  31. Chaleff RS (1983 ) Isolation of agronomically useful mutants from plant cell culture. Science 219 :676–682PubMedCrossRefGoogle Scholar
  32. Chaleff RS, Stolarz A (1982) The development of anther culture as a system for in vitro mutant selection. In: Rice tissue culture planning Conf, IRRI, Manila, pp 63–74Google Scholar
  33. Chang T-T (1985) Crop history and genetic conservation: rice — a case study. Iowa State J Res 59:425–455Google Scholar
  34. Chang TT, Morishima H, Huang CS, Tagumpay O, Tateno K (1965) Genetic analysis of plant height, maturity and other quantitative traits in the cross of Peta x I-Geo-Tze. J Agric Assoc China 51:1–8Google Scholar
  35. Chen C-C (1984) Utilization of microspore-derived plants for genetic analysis in rice. Rice Genet Newslett 1:137–138Google Scholar
  36. Chen C-C, Chen C-M (1980) Changes in chromosome number in microspore callus of rice during successive subculture. Can J Genet Cytol 22:607–614Google Scholar
  37. Chen C-C, Chiu W-L, Yu L-J. Ren S-S, Yu W-J (1983) Genetic analysis of anther-derived plants of rice: independent assortment of unliked genes. Can J Genet Cytol 25:324–328Google Scholar
  38. Chen C-C, Tsay H-S, Huang C-R (1986) Rice (Oryza sativa L.): factors affecting androgenesis. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 2. Crops I. Springer, Berlin Heidelberg New York Tokyo, pp 123–138Google Scholar
  39. Chen C-M, Chen C-C (1979) Selection and regeneration of 5-methyltryptophan-resistant rice plants from pollen callus. Natl Sci Counc Month 7:378–382Google Scholar
  40. Chen C-M, Chen C-C, Lin M-H (1982) Genetic analysis of anther derived plants ofrice. J Hered 73 :49–52Google Scholar
  41. Chen D-F, Hsia Z-A (1987) Plant regenerated from protoplast of Polypogon fogax Nees ex Steud. Sci Sin B 30:54–60Google Scholar
  42. Chen J-J, Chen L-J, Tsay H-S (1984) Relationship between the time of callus initiation, callus age and the ability of organ regeneration from rice anther culture. J Agric Assoc China 126:2–9Google Scholar
  43. Chen L-J, Luthe DS (1985) Protein and RNA synthesis in embryogenic and non-embryogenic callus of rice (Oryza sativa L.) In: Abstr 1st Int Congr Plant molecular biology, Univ Georgia, USA, p 103Google Scholar
  44. Chen S-L, Tian W-Z, Zhang G-H (1983) Selection of NaCl-tolerant regenerated plants from rice pollen-derived calli. Chin J Cell Biol (Spacil):29–30Google Scholar
  45. Chen Y (1986a) Anther and pollen culture of rice. In: Hu H, Yang H-Y (eds) Haploids of higher plants in vitro. Science Press, Beijing, pp 3–25Google Scholar
  46. Chen Y (1986b) The inheritance of rice pollen plant and its application in crop improvement. In: Hu H, Yang HY (eds) Haploids of higher plants in vitro. Science Press, Beijing, pp 118–136Google Scholar
  47. Chen Y, Li L-C, Zhu J, Wang R-F, Li S-Y, Tian W-Z, Zeng S-W (1974) Investigation on the induction and genetic expression of rice pollen plants. Sci Sin 17:209–226Google Scholar
  48. Chen Y, Wang R-F, Tian W-Z, Zuo Q-X, Zheng S-W, Lu D-Y, Zhang G-H (1980) Studies on pollen culture in vitro and induction of plantlets in Oryza sativa L. subsp. Keng. Acta Genet Sin 7:46–54Google Scholar
  49. Chen Y, Zuo Q-X, Li S-Y, Zheng S-W (1981) Green plants regenerated from isolated rice pollen grains in vitro and induction factors. Acta Genet Sin 8:158–163Google Scholar
  50. Chowdhury MKU, Schaeffer GW, Smith RL, Matthews BF (1988) Molecularanalysis oforganelle DNA of different subspecies of rice and the genetic stability of mtDNA in tissue cultured cells of rice. Theor Appl Genet 76:533–539Google Scholar
  51. Chu C-C, Wang C-C, Sun C-S (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Asi Sin 18:659–668Google Scholar
  52. Chu Q-R, Zhang L-N (1985) Cytogenetics of aneuploids derived from pollen plants of rice. Acta Genet Sin 12:51–60Google Scholar
  53. Chu Q-R, Zhang Z-H, Gao Y-H (1985) Cytogenetical analysis on aneuploids obtained from pollen clones of rice (Oryza sativa L.) Theor Appl Genet 71:506–512Google Scholar
  54. Chu Q-R, Cao H-X, Gu Y-Q, Zhang Z-H (1986) Stem node culture in vitro of twelve species of wild rice and two distant hybrids. Acta Agric Shanghai 2:39–46Google Scholar
  55. Cocking EC, Abdullah R, Thompson JA (1986) Procedures for the efficient regeneration of rice plants from protoplasts. Rockefeller Found Plann Meet, Oct 1986. IRRI, ManilaGoogle Scholar
  56. Constantin MT (1981) Chromosome instability in cell and tissue cultures and regenerated plants. Environ Exp Bot 21:359–368CrossRefGoogle Scholar
  57. Coulibaly MY, Demarly Y (1986) Regeneration of plantlets from protoplast of rice. Z Pflanzenzücht 96:79–81Google Scholar
  58. Croughan TP (1984) Tissue culture of US rice varieties: development of short stature variants through tissue culture. In: Abstr Int Symp Genetic manipulation of crops, Oct 22–26, 1984, Beijing, p 57Google Scholar
  59. D’Amato F (1977) Cytogenetics of differentiation in tissue and cell culture. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell. tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 343–357Google Scholar
  60. D’Amato F (1978) Chromosome number variation in cultured cells and regenerated plants. In: Thorpe TA (ed) Frontiers of plant tissue culture. Univ Calgary, Can, pp 287–295Google Scholar
  61. Dattee Y, San H, Andre I, Odjo A, Kouaddio (1984) Quantitative inheritance of difference between sexual self pollinated, androgenetic and gynogenetic lines in rice and barley. In: Abstr Int Symp Genetic manipulation in crops, Oct 22–26, 1984, Beijing, p 31Google Scholar
  62. Davoyan EI (1981) Induction of callus and differentiation of plants from rice endosperm. Dokl BACXHUL (USSR) (6):43–45Google Scholar
  63. Davoyan EI (1983) Mutagenesis in rice tissue culture and obtaining a new initial material on its basis. Genetics (USSR) 19:1714–1719Google Scholar
  64. Davoyan EI (1986) On hormonal regulation of morphogenetic potency in long-term cultivated callus tissues of rice. Agric Biol (USSR) 21:85–89Google Scholar
  65. Davoyan EI, Smetanin AP (1979) Callus induction and rice plant regeneration. Plant Physiol Moscow 26:323–329Google Scholar
  66. Day A, Ellis THN (1984) Chloroplast DNA deletions associated with wheat plants regenerated from pollen: possible basis for maternal inheritance of chloroplasts. Cell 39:359–368PubMedCrossRefGoogle Scholar
  67. Deka C, Sen SK (1976) Differentiation in calli originated from isolated protoplasts of rice (Oryza sativa L.) through plating technique. Mol Gen Genet 145 :239–243Google Scholar
  68. Earle ED, Gracen VE (1985) Somaclonal variation in progeny of plants from tissue cultures. In: Henke RR, Hughes KW , Constantin MJ, Hollaender A (eds) Tissue culture and agriculture. Plenum, New York, pp 139–152Google Scholar
  69. Edallo S, Zucchimali C, Perenzin M, Salamini F (1981) Chromosomal variation and frequency of spontaneous mutation associated with in vitro culture and plant regeneration in maize. Maydica 26 :29–56Google Scholar
  70. Evans DA, Sharp WR (1983) Single gene mutations in tomato plants regenerated from tissue culture. Science 221:949–951PubMedCrossRefGoogle Scholar
  71. Evans DA, Sharp WR, Madina-Filho HP (1984) Somaclonal and gamatoclonal variation. Am J Bot 71:759–774CrossRefGoogle Scholar
  72. FAO (ed) (1986) Selected indicators of food and agriculture development in Asia-Pacific region 1975–1985. RAPA, Bangkok, 1986/14Google Scholar
  73. FAO Production Year Book, 1988, Vol 41Google Scholar
  74. Finkle BJ, Ulrich JM (1982) Cryoprotectant removal temperature as a factor in the survival of frozen rice and sugarcane cells. Cryobiology 19:1012–1018CrossRefGoogle Scholar
  75. Finkle BJ, Ulrich JM, Tisserat B (1982) Responses of several lines of rice and date palm callus to freezing at -196’C. In: Li PH, Sakai A (eds) Plant cold hardiness and freezing stress. Academic Press, New York London, pp 643–660Google Scholar
  76. Fujimura T, Sakurai M, Akagi H, Negishi T, Hirose A (1985) Regeneration of rice plants from protoplasts. Plant Tissue Cult Lett 2:74–75CrossRefGoogle Scholar
  77. Fujiwara T, Ojima (1955) Ref.: Oono K (1984) Tissue culture and genetic engineering in rice. In: Tsunoda S, Takahashi W (eds): Biology of Rice. Japan Sci Soc Press Tokyo, pp 339–358Google Scholar
  78. Fukui K (1983) Sequential occurrence of mutations in a growing rice callus. Theor Appl Genet 65:225–230CrossRefGoogle Scholar
  79. Furuhashi K, Yatazawa M (1964) Indefinite culture of rice stem node callus. Kagaku (Jpn) 34:623Google Scholar
  80. Furuhashi K, Yatazawa M (1970) Methionine-lysins-threonine-isoleucine interrelationships in the amino acid nutrition of rice callus tissue. Plant Cell Physiol 11:569–578Google Scholar
  81. Guha S, Iyer RD, Gupta N, Swaminathan MS (1970) Totipotency of gametic cells and the production of haploids in rice. Curr Sci 39:174–176Google Scholar
  82. Guha-Mukherjee S (1972) Genotypic differences in the in vitro formation of embryoids from rice pollen. J Exp Bot 24:139–144CrossRefGoogle Scholar
  83. Hasegawa H, Inoue M (1983) Induction and selection of hydroxy-L-proline resistant mutant in rice (Oryza sativa L.) Jpn J Breed 33:275–282Google Scholar
  84. Heinz DJ, Krishnanurthi M, Nickell LG, Maretzki A (1977) Cell, tissue and organ culture in sugarcane improvement. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 3–17Google Scholar
  85. Henke RR, Mansur MA, Constantin MJ (1978) Organogenesis and plant formation from organ and seedling derived calli of rice (Oryza sativa L.) Physiol Plant 44:11–14CrossRefGoogle Scholar
  86. Henshaw GG, O’Hara JF (1983) In vitro approaches to the conservation and utilisation of global plant genetic resources. In: Mantell SH, Smith H (eds) Plant biotechnology. Univ Press, Cambridge, pp 219–238Google Scholar
  87. Heszky LE, Sajo Z (1972) Raising haploid plants of Oryza sativa L. by in vitro anther and ovary culture. I. callus-tissue root and shoot induction. Agrobotenika 14:35–41Google Scholar
  88. Heszky LE, Li SN (1984) Effect of callus subculture on plant regeneration capacity in somatic and haploid callus of Oryza sativa L. In: Novak FJ, Havel L, Dolezel J (eds) Plant tissue and cell culture application to crop improvement. Czech Akad Sci, Prague. pp 123–124Google Scholar
  89. Heyser JW, Dykes TA, DeMott KJ, Nabors MW (1983) High frequency, long term regeneration of rice from callus culture. Plant Sci Lett 29:175–182CrossRefGoogle Scholar
  90. Hibberd KA, Green CE (1982) Inheritance and expression of lysine plus threonine resistance selected in maize tissue culture. Proc Natl Acad Sci USA 79:559–563PubMedCrossRefGoogle Scholar
  91. Hildreth G, Thompson M, Hanning G, Nabors M (1987) Plant regeneration from long-term cultures derived from mature seeds of Indica and Japonica rice culture. In: Proc 2nd Annu Conf International plant biotechnology network (IPBNet), Jan 11–16, 1987, BangkokGoogle Scholar
  92. Hu Z, Zhuang C-J, Liang H-X (1983) In vitro culture of haploid young panicle fragments of rice. Acta Bot Yunnanica (China) 5:293–300Google Scholar
  93. Huang C-S, Tsay H-S, Chen C-G (1985) Genetic changes in rice variety induced by anther culture J Agric Res China 34:237–242Google Scholar
  94. Huang Y-X, Liu J-L, Hu J-Y, Ke W (1960) Advances in rice breeding in Guadong Province during 1949–1959. Acta Agric Sin 11:20–29Google Scholar
  95. International Rice Research Institute-IRRI (ed) (1967) Annual report for 1966. IRRI, Manila, pp 59–82Google Scholar
  96. Ishikawa R, Mori K, Morishima H, Kinoshita T (1987) Expression pattern of isozyme genes in rice calli. Memoirs of the Faculty of Agriculture, Hokkaido Univ 15:371–379Google Scholar
  97. Iyer RD, Govila QP (1964) Embryo culture of interspecific hybrids in the genus Oryza. Indian J Genet Plant Breed 24:116–121Google Scholar
  98. Jena KK, Khush GS (1984) Embryo rescue of interspecific hybrid and its scope in rice improvement. Int Rice Genet Newslett 1:133–134Google Scholar
  99. Jena KK . Khush GS (1986) Production of monosomic alien addition lines of Orvza sativa having a single chromosome of O. officinalis. In: Rice Genetics. Proc Int Rice Genetics Symp IRRI, Manila, Philippines. pp 199–208Google Scholar
  100. Jordan MC, Larter EN (1985) Somaclonal variation in triticale ( x Triticosecale Wittmack) cv. Carman. Can J Genet Cytol 27:151–157Google Scholar
  101. Jun C. Rush MC. Croughan T (1986) The improvement of rice through somaculture. In: Abstr 6th Int Cong Plant tissue and cell culture, Aug 3–8, 1986. Univ Min, Minneapolis. p 228Google Scholar
  102. Karp A, Maddock SE (1984) Chromosome variation in wheat plant regenerated from cultured immature embryos. Theor Appl Genet 67:249–255CrossRefGoogle Scholar
  103. Kawata S, Ishihara A (1968) The regeneration of rice plant, Orvza sativa L. in the callus derived from the seminal root. Proc Jpn Acad Sci 44:149–153Google Scholar
  104. Kharchenko PN, Kucherenko LA (1977) Obtaining rice plants from anthers. Dokl BACXHUL (USSR) (4):15–16Google Scholar
  105. Khush GS (1984) A lecture on rice breeding presented at CNRRI. China Natl Rice Res Inst, Hangzhou, China, pp 1–2Google Scholar
  106. Khush GS, Virmani SS (1985) Some plant breeding problems needing biotechnology. In: Biotechnology in international agricultural research. IRRI, Manila, pp 51–63Google Scholar
  107. Kishikawa H, Takagi Y, Egashira M, Yamashita H, Takamori Y (1986) Morphological variation in the progenies of plants regenerated from anther culture of tetraploid rice. Bull Fac Agric Saga Univ, Jpn (6):37–48Google Scholar
  108. Kishinami I, Ojima K (1980) Accumulation of r-aminobutyric acid due to adding ammonium or glutamine to cultured rice cells. Plant and Cell Physiology 21:581–589Google Scholar
  109. Kishor PBK, Reddy GM (1985) Resistance of rice callus tissues to sodium chloride, and polyethylene glycol. Current Science, India 54:1129–1131Google Scholar
  110. Kishor PBK, Reddy GM (1986a) Regeneration of plants from long term cultures of Oryza sativa L. Plant Cell Rep 5 :391–393CrossRefGoogle Scholar
  111. Kishor PBK, Reddy GM (1986b) Osmotic adjustment and organogenesis in long term culture of rice. In: Abstr 6th Int Congr Plant tissue and cell culture, Aug 3–8, 1986, Univ Minn, Minneapolis, p 159Google Scholar
  112. Kishor PBK, Reddy GM (1986c) Improvement of rice for tolerance to salt and drought through tissue culture. Oryza 23 :102–108Google Scholar
  113. Kostylev PI, Yatsyna AA (1986) Use of embryo culture in rice interspecific crossings. Dokl BACXHIL (USSR) (5): 11–12Google Scholar
  114. Kucherenko LA (1980) Tissue culture in rice improvement: experiences in the USSR. In: Innovative approaches to rice breeding. IRRI, Manila, pp 93–102Google Scholar
  115. Kucherenko LA (1984a) Conditions for plant regeneration in tissue culture. Agric Biol (USSR) (4):70–72Google Scholar
  116. Kucherenko LA (1984b) Obtaining of somaclonal variation in rice tissue culture. In: Abstr Int Symp Genetic manipulation in crops, Oct 22–26, Beijing, p 53Google Scholar
  117. Kucherenko LA (1986) Reproduction of non-germinating rice seed by tissue culture method. Agric Biol (USSR) (4):14–17Google Scholar
  118. Kucherenko LA, Loce GD (1982) Accelerate the breeding cycle with embryo culture. Selekt Semenov (10):19–20 (in Russian)Google Scholar
  119. Kucherenko LA, Mammaeva GG (1979) Study of regenerated rice plants obtained by tissue culture. Docl BACXHUL (USSR) (3):10–12Google Scholar
  120. Kucherenko LA, Mammaeva GG (1980) Callus formation and organogenesis in tissue culture of different rice varieties. Agric Biol (USSR) 15:384–386Google Scholar
  121. Kuo M-K, Cheng W-C, Hwang T-N, Hwang J-S, Kuan Y-L (1977) The culture of pollen grain of wheat, rice and tobacco in vitro. Acta Genet Sin 4:333–338Google Scholar
  122. Kuo S-C (1982) The preliminary studies on culture of unfertilized ovary of rice in vitro. Acta Bot Sin 24 :33–38Google Scholar
  123. Kyozuka J, Otoo E, Shimamoto K (1988) Plant regeneration from protoplasts of indica rice: genotypic differences in culture response. Theor Appl Genet 76:887–890CrossRefGoogle Scholar
  124. Lai K-L, Liu L-F (1982) Induction and plant regeneration ofcallus from immature embryos ofrice plants (Oryza sativa L.) Jpn J Crop Sci 51:70–71CrossRefGoogle Scholar
  125. Lai K-L, Liu L-F (1986) High frequency plant regeneration in water- and salt-stressed rice cell culture. In: Abstr 6th Int Cong Plant tissue and cell culture, Aug 3–8, 1986, Univ Minn, Minneapolis, p.186Google Scholar
  126. Lai L-Z (1982) Plantlet derived from young preembryos of rice. Kexue Tongbao (Beijing) 27:1334–1337Google Scholar
  127. Lai L-Z (1986) A promising line derived from ovary culture of rice. In: Abstr Pap Pres 3rd Natl Meet Chinese Soc Cell biology, Chengdu, China, pp 141–142Google Scholar
  128. Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell culture for plant improvement. Theor Appl Genet 60:197–214CrossRefGoogle Scholar
  129. Lei M, Li X-H, Sun Y-R, Huang M-J (1986) Plantlet regeneration from rice protoplasts. Kexue Tongbao (China) 31:1729–1731Google Scholar
  130. Li C-C, Gan D. Xu J (1986) New progress on screening resistant mutant of rice in vitro. Sci Agric Sin (2) :93–94Google Scholar
  131. Li H-M, Chen Y (1987) Selection of cadmium tolerant mutants from rice anther culture. Acta Genet Sin 14:42–48Google Scholar
  132. Li H-W, Weng T-S, Chen C-C, Wang W-H (1961) Cytological studies of Oryza sativa L. and its related species. Bot Bull Acad Sin 2:79–86Google Scholar
  133. Li SN, Heszky LE (1986) Rice tissue culture and application to breeding. I. Induction of high totipotent haploid and diploid callus from the different genotypes of rice (Oryza sativa L.) Cereal Res Commun 14:197–203Google Scholar
  134. Ling D-H, Wang X-H, Chen M-F (1981) Cytogenetical study on homologous asyndetic triploid derived from anther culture in rice. Acta Genet Sin 8:262–268Google Scholar
  135. Ling D-H, Chen W-Y, Chen M-F, Ma Z-V (1984) A study of induction and plantlet differentiation of embryogenic cluster of trihaploid rice. Acta Genet Sin 11:26–32Google Scholar
  136. Ling D-H, Vidhyasekaran P, Borromeo ES, Zapata FJ, Mew TW (1985) In vitro screening of rice germplasm for resistance to brown spot disease using phytotoxin. Theor Appl Genet 71:133–135CrossRefGoogle Scholar
  137. Ling D-H, Chen W-Y, Chen M-F, Ma Z-R (1987) Chromosomal variation of regenerated plants from somatic cell culture in indica rice. Acta Genet Sin 14:249–254Google Scholar
  138. Ling S-C, Min S-K, Huang Y-X, Zu D-M (1986) Cross-breeding of rice. In: Rice cultivation science in China. CAAS Agronomic Press, Beijing, pp 271–330Google Scholar
  139. Liu L-F, Lai K-L (1985) High frequency plant regeneration from water-stressed rice tissue cultures. In: Abstr lst Int Congr Plant molecular biology, Univ Georgia, p 11Google Scholar
  140. Liu Z-L, Zhou C (1984) Investigation of ploidy and other characters of the gynogenic plants in Oryza sativa L. Acta Genet Sin 11:113–119Google Scholar
  141. Loo S-W, Xu Z-H (1986) Rice: anther culture for rice improvement in China. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 2. Crops I. Springer, Berlin Heidelberg New York Tokyo, pp 139–156Google Scholar
  142. Lu C, Vasil V, Vasil IK (1981) Isolation and culture of protoplast of Panicum maximum Jacq (guinea grass): somatic embryogenesis and plantlet formation. Z Pflanzenphysiol 104:311–318Google Scholar
  143. Lu J-J, Chang T-T (1980) Rice in its temporal and spatial perspectives. In: Luh BS (ed) Rice: production and utilization. AVI, Westport, Conn, pp 1–74Google Scholar
  144. Maeda E (1965) Callus formation and isolation of single cells from rice seedling. Proc Crop Sci Soc Jpn 34:369–376CrossRefGoogle Scholar
  145. Maeda E (1968) Subculture and organ formation in the callus derived from rice embryos in vitro. Proc Crop Sci Soc Jpn 37:51–58CrossRefGoogle Scholar
  146. Maeda E (1971) Isolation of protoplast from seminal roots of rice. Proc Crop Sci Soc Jpn 40:397–398CrossRefGoogle Scholar
  147. McKenzie KS, Croughan TP (1984) Cool temperature seedling vigor of R2 and M2 short-staure rice (Orvza sativa L.) In: Abstr Int Symp Genetic manipulation in crops, Oct 22–26, 1984 Reiiing n 57Google Scholar
  148. Meng Z, Chen Y (1987) Screening of rice mutant resistant to amino acid analogs. I. Screening of a rice mutant resistant to S-(2-aminoethyl)L-cysteine. Acta Genet Sin 14:100–106Google Scholar
  149. Mercy ST, Zapata FJ (1986) Chromosomal behavior of anther culture derived plants of rice. Plant Cell Rep 5:215–218CrossRefGoogle Scholar
  150. Metakovsky EV, Yu Novoselskay A, Sozinov AA (1987) Problems of interpreting results obtained in studies of somaclonal variation in gliadin proteins in wheat. Theor Appl Genet 73:764–766CrossRefGoogle Scholar
  151. Miah MAA, Earle ED, Khush GS (1985) Inheritance of callus formation ability in anther culture of rice, Oryza sativa L. Theor Appl Genet 70:113–116Google Scholar
  152. Mocoy TJ, Phillips PL, Rines HW (1982) Cytogenetic analysis of plants regenerated from oat (Avena sativa) tissue cultures: high frequency of parial chromosome loss. Can J Genet Cytol 24:37–50Google Scholar
  153. Mori K (1986) Callus induction and protoplast culture in rice. In: Rice genetics. IRRI, Manila, Philippines, pp 781–790Google Scholar
  154. Nagai C, Schnell RJ (1986) Comparison of F2 population and somaclones derived from a hybrid of Saccharum x Erintus (Ripidium). In: Abstr 6th Int Congr Plant tissue and cell culture, Aug 3–8, 1986, Univ Minn, Minneapolis, p 218Google Scholar
  155. Nakajima T, Morishima H (1958) Studies on embryo culture in plants. II. Embryo culture of interspecific hybrids of Oryza. Jpn J Breed 8:105–110Google Scholar
  156. Nakano H, Tashiro T, Maeda E (1975) Plant differentiation in callus tissue induced from immature endosperm of Oryza sativa L. Z Pflanzenphysiol 76:444–449Google Scholar
  157. Nelson RS, Karp A, Bright SWJ (1986) Ploidy variation in Solanum brevideus plants regenerated from protoplasts using an improved culture system. J Exp Bot 37:253–261CrossRefGoogle Scholar
  158. Niizeki H (1968) Tissue culture of rice. KASEAA (Jpn) 6:743–748Google Scholar
  159. Niizeki H, Kita F (1981) Cell division of rice and soybean and their fused protoplasts. Jpn J Breed 31:161–167Google Scholar
  160. Niizeki H, Oono K (1968) Induction of haploid rice plant from anther culture. Proc Jpn Acad Sci 44:554–557Google Scholar
  161. Niizeki H, Oono K (1971) Rice plants obtained by anther culture. Cult Tissue Plants 193:251–257Google Scholar
  162. Niizeki H, Tanaka M, Saito K (1986) Response of somatic hybrid callus between rice and soybean to streptomycin. Jpn J Breed 36:75–79Google Scholar
  163. Nishi T, Mitsuoka S (1969) Occurrence of various ploidy plants from anther and ovary culture of rice plant. Jpn J Genet 44:341–346CrossRefGoogle Scholar
  164. Nishi T, Yamada Y, Takahashi E (1968) Organ redifferentiation and plant regeneration in rice callus. Nature (London) 219:508–509CrossRefGoogle Scholar
  165. Nowick EM (1986) Chromosome pairing in Oryza sativa L. x O. latifolia Desv. hybrid. Can J Genet Cytol 28:278–281Google Scholar
  166. Nunez Z, VM Martinez CP, Narvaez VJ, Roca W(1985) Obtaining homozygous lines ofrice (Oryzasativa L.) tolerant of toxic Al by the use of anther culture. Acta Agron 35:7–26Google Scholar
  167. Oono K (1978) Test tube breeding of rice by tissue culture. Trop Agric Res Ser 11:109–124Google Scholar
  168. Oono K (1981) In vitro methods applied to rice. In: Thorpe TA (ed) Plant tissue culture, methods and application in agriculture. Academic Press, New York London, pp 273–298Google Scholar
  169. Oono K (1983) Genetic variability in rice plants regenerated from cell culture. In: Cell and tissue culture techniques for cereal crop improvement. Science Press, Beijing, IRRI, Manila, pp 95–104Google Scholar
  170. Oono K (1984) Regulation of somatic mutation in rice tissue culture. In: Abstr Int Symp Genetic manipulation of crops, BeijingGoogle Scholar
  171. Oono K (1985) Tissue culture application in rice improvement. In: Int Rice research conference. IRRI, ManilaGoogle Scholar
  172. Oono K (1986) Somatic mutation in rice cultured cell. Tissue Cult Jpn 12:288–293Google Scholar
  173. Oono K, Okuno K, Kawai T (1984) High frequency of somaclonal mutations in callus culture of rice. Gamma Field Symp 23 : 71–95Google Scholar
  174. Oram R (1982) Plant breeding and genetic engineering. In: Sutton BG, Williams P (eds) Genetic engineering for agriculture — the substance behind the promise. Aust Inst Agric Occ Publ 2, pp 73–79Google Scholar
  175. Orton TT (1980) Chromosomal variation in tissue cultures and regenerated plants of Hordeum. Theor Appl Genet 56 :101–112CrossRefGoogle Scholar
  176. Ou-Lee TM, Turgeon R, Wu R (1986) Expression of a foreign gene linked to either a plant-virus or a Drosophila promotor after electroporation of protoplasts of rice, wheat, and sorghum. Proc Natl Acad Sci USA 83:6815–6819PubMedCrossRefGoogle Scholar
  177. Paul NK, Ghosh PD (1986) In vitro selection of NaCI tolerant cell cultures in Oryza sativa L. Curr Sci 55:568–569Google Scholar
  178. Pulver EL, Jennings PR (1986) Application of anther culture to high volume rice breeding. In: Rice genetics, IRRI, Manila, pp 811–820Google Scholar
  179. Qin R-Z, Wu D-Y, Chen Z-V, Song W-C, Zhang Y-H, Dao W-K (1984) Investigation on the autoteraploid rice clones derived from the elite plants of hybrid progenies. In: Abstr Int Symp Genetic manipulation in crops, Oct 22–26, 1986, BeijingGoogle Scholar
  180. Raghava RV, Nabors MW (1984) Cytokinin mediated long-term, high-frequency plant regeneration in rice tissue culture. Z Pflanzenphysiol 113:315–323Google Scholar
  181. Raghava RV, Nabors MW (1985) Plant regeneration from tissue culture of Pokkali rice is promoted by optimizing callus to medium volume ratio and by a medium-conditioning factor produced by embryogenic callus. Plant Cell Tissue Org Cult 4:241–248CrossRefGoogle Scholar
  182. Rains DW , Croughan TP, Stavarek SJ (1982) Selection of salt tolerant plants using tissue culture. In: Rains DW, Valentine RC, Hollaender A (eds) Genetic engineering of osmoregulation: impact on plant productivity for food, chemicals and energy. Plenum, New York, pp 279–292Google Scholar
  183. Reddy GM (1983) Callus initiation and plant regeneration from haploid internodes in rice. In: Sen SK, Giles KL (eds) Plant cell culture in crop improvement. Plenum, New York, pp 113–118Google Scholar
  184. Reddy GM (1986) Callus induction, somatic embryogenesis and plantlet regeneration from young inflorescences of rice and maize. In: Abstr 6th Int Congr Plant tissue cell culture, Aug 3–8, 1986, Univ Minn, Minneapolis, p 444Google Scholar
  185. Rutger JN, Peterson ML (1976) Improved short stature rice. Cal Agric 30 (6):4–6Google Scholar
  186. Saka H, Maeda E (1973) Characteristics and variated differences of amylase isozymes in rice callus tissues. Proc Crop Sci Jpn 42:307–314CrossRefGoogle Scholar
  187. Saka H, Maeda E (1974) Changes in some hydrolytic enzymes associated with the redifferentiation of shoots in rice callus tissues. Proc Crop Sci Jpn 43:207–218CrossRefGoogle Scholar
  188. Sala F, Cella R, Burroni D, Rollo F(1979) Freeze preservation of rice cells grown in suspension culture. Physiol Plant 45:170–176Google Scholar
  189. Sano H, Takaiwa F, Oono K (1983) Induction of mutation tissue culture and their use for plant breeding IX. variation of seed protein and amino acid (lysine) in regenerated plants. Jpn J Breed 33(Suppl 2):84–85Google Scholar
  190. Schaeffer G W (1982) Recovery of heritable variability in anther-derived doubled haploids of rice. Crop Sci 22:1160–1164CrossRefGoogle Scholar
  191. Schaeffer GW (1986) Selection of rice, Oryza sativa, cells from inhibitory levels of lysine plus threonine and the characterization ofthe progeny. In: Abstr 6th Int Congr Plant cell tissue culture, Aug 3–8,1986, Univ Minn, Minneapolis, p 439Google Scholar
  192. Schaeffer GW, Sharpe FT Jr (1981) Lysine in seed protein from S-aminoethyl-L-cysteine resistant anther-derived tissue cultures of rice. In Vitro 17:345–352Google Scholar
  193. Schaeffer GW, Sharpe FT Jr (1983) Improved rice proteins in plants regenerated from S-AEC-resistant callus. In: Cell and tissue culture techniques for cereal crop improvement. Science Press, Beijing; IRRI, Manila, pp 279–290Google Scholar
  194. Schaeffer GW, Sharpe FT Jr, Cregan PB (1984) Variation for improved protein and yield from rice anther culture. Theor Appl Genet 67:383–389CrossRefGoogle Scholar
  195. Schaeffer GW, Sharpe FT Jr, Carnahan HL, Johnson CW (1986) Anther and tissue culture — induced grain chalkiness and associated variants in rice. Plant Cell Tissue Org Cult 6:149–157CrossRefGoogle Scholar
  196. Sharma DR, Dawra S, Chowdhury JB (1983) Direct and indirect effects of gamma rays on stimulation of morphogenesis in longterm tissue culture of rice (Oryza sativa L.) Curr Sci 52:606–607Google Scholar
  197. Sharma KD (1986) Induced mutagenesis in rice. In: Rice genetics. IRRI,, Manila, pp 679–695Google Scholar
  198. She J-M, Sun L-H, Huang M (1984) Differentiation potential and chromosome variation in subcultured callus derived from rice pollen. Hereditas (China) 6:17–19Google Scholar
  199. Shen J-H, Li M-F, Chen Y-Q, Zhang Z-H (1983) Improving rice by anther culture. In: Cell and tissue techniques for cereal crop improvement. Science Press, Beijing; IRRI, Manila, pp 183–205Google Scholar
  200. Shen Q-J (1985) Variations in the characteristics of plant progenies derived frdm somatic tissue (D2) of indica rice. Plant Physiol Commun (China) (2):35–37Google Scholar
  201. Shepard JF, Bidney D, Shahin E (1980) Potato protoplasts in crop improvement. Science 208:17–24PubMedCrossRefGoogle Scholar
  202. Shimamoto K, Hayashi Y, Kyozuka J (1986) High frequency plant regeneration from protoplasts of rice (Oryza sativa L.) In: Abstr 6th Int Congr Plant tissue cell culture, Aug 3–8, 1986, Univ Minn, Minneapolis, p 161Google Scholar
  203. Shimamoto K (1988) Genetic modifications of higher plants using cell cultures. Plant Tissue Culture Letter 5:1–5CrossRefGoogle Scholar
  204. Shome A, Bhaduri PN (1982) Response of excised embryos of rice (Oryza sativa L.) to X-rays. Theor Appl Genet 61:135–139CrossRefGoogle Scholar
  205. Song Z-M, Liu Z-L, Chen J-X (1984) Screening of ideotypes from somatic tissue-derived plants of rice. In: Abstr Int Symp Genetic manipulation in crops, Oct 22–26, 1984, Beijing, p 61Google Scholar
  206. Song Z-M, Liu Z-L, Chen J-X (1986) Somaclonal variation in rice improvement. Anhuei Agron Sci (China) 4:34–37Google Scholar
  207. Suenaga K, Abrigo EM, Yoshida S (1982) Seed derived callus culture for selecting salt-tolerant rice. I. Callus induction, plant regeneration, and variations in visible plant traits. IRRI Res Pap Ser 79:1–11Google Scholar
  208. Sun C-S, Wang C-C, Chu C-C (1974) The ultrastructure of plastids in the albino pollen plants of rice. Sci Sin 17:793–802Google Scholar
  209. Sun C-S, Wang C-C, Chu C-C (1979) The deficiency of soluble proteins and plastid ribosome RNA in the albino pollen plantlets of rice. Theor Appl Genet 55:193–197CrossRefGoogle Scholar
  210. Sun L-H, She J-M (1982) Induction of diploid plant from somatic cell culture in haploid plant of rice. Hereditas (Beijing) (4):9Google Scholar
  211. Sun L-H, She J-M, Lu X-F (1986) In vitro selection of Xanthomonas oryzae-resistant mutants in rice. I. Induction of resistant callus and screening regenerated plants. Acta Genet Sin 13:188–193Google Scholar
  212. Sun Z-X (1980) Observation on the regenerated plants from somatic tissue of hybrid rice. Acta Phytophysiol Sin 6:243–249Google Scholar
  213. Sun Z-X, Colbert J (1987) Somaclonal variation in the electropherograms of protein of rice. In: Abstracts of the Symposium on the Plant Cell Biology. 26, Oct - 1, Nov. 1987, Dayun, HunanGoogle Scholar
  214. Sun Z-X, Zhao C-Z, Zheng K-L, Qi X-F, Fu Y-P (1981) Observation on the regenerated plants from somatic tissue of hybrid rice in paddy field. Acta Phytophysiol Sin 7:161–166Google Scholar
  215. Sun Z-X, Zhao C-Z, Zheng K-L, Qi X-F, Fu Y-P (1983) Somaclonal genetics of rice, Oryza sativa L. Theor Appl Genet 67:67–73CrossRefGoogle Scholar
  216. Sun Z-X, Fu Y-P, Zhang Y-X (1988a) A preliminary research on long term storage of rice germplasm in vitro. Sci Agric Sin 21(4):9–14Google Scholar
  217. Sun Z-X, Xie F-X, Rong J-P (1988b) The correlation analysis on agronomic characteristics between somaclonal generations of rice. In: Abstracts of the Papers Presented at the 55th Anniversary of the Botanical Society of China. August, 1988, Kwenming, China, p 410Google Scholar
  218. Sun Z-X, Luo Y-K, Zhang Y-K, Chen Y-Y (1989) Somaclonal variation in grain quality ofrice. Hereditas (Beijing), 11(5):5–8Google Scholar
  219. Taiwan Agricultural Research Institute (ed) (1986) Annual report of Taiwan Agricultural Research Institute, pp 19–20Google Scholar
  220. Takagi Y, Kishikawa H, Egashira Y (1982) Anther culture of tetraploid rice. Bull Fac Agric Saga Univ Jpn 52:41–47Google Scholar
  221. Tarnura S (1968) Shoot formation in callus originated from rice embryo. Proc Jpn Acad Sci 44:544–548Google Scholar
  222. Tang Y-Y, Sun Z-X (1979) In vitro regeneration of plantlets from the tissue of hybrid rice. Acta Phytophysiol Sin 5:95–97Google Scholar
  223. Terada R, Kyozuka J, Nishibayashi S, Shimamoto K (1987) Plantlet regeneration from somatic hybrids of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola Vasing). Mol Gen Genet 210:39–43CrossRefGoogle Scholar
  224. Thompson JA, Abdullah R, Cocking EC (1986) Protoplast culture of rice using media solidified with agarose. Plant Sci 47:123–133CrossRefGoogle Scholar
  225. Thompson JA, Abdullah R, Chen W-H, Gartland KMA (1987) Enhanced protoplast division in rice following heat shock treatment. J Plant Physiol 127:363–370CrossRefGoogle Scholar
  226. Tomes DT (1985) Cell culture, somatic embryogenesis and plant regeneration in maize, rice, sorghum and millets. In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture. NijoffJunk, Dordrecht, pp 175–203CrossRefGoogle Scholar
  227. Tonnelli C (1985) In vitro selection and characterization of cereal mutants. Genet Agric 39:445–470Google Scholar
  228. Toriyama K, Hinatak K (1986) Plantlet regeneration from protoplasts of rice. Heredity (Jpn) 40:32–37Google Scholar
  229. Toriyama K, Arimoto Y, Uchimiya H, Hinata K (1988) Transgenic rice plants after direct gene transfer into protoplasts. Biotechnology 5:1072–1074Google Scholar
  230. Tsai I-S (1978) A preliminary report on callus induction and plant regeneration from the shoot apex of hybrid rice in Oryza sativa L. In: Proc Symp Plant tissue culture. Science Press, Beijing, pp 517–519Google Scholar
  231. Uchimiya H, Fushima T, Hashimota H, Harada H, Syono K, Sugawara Y (1986) Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa L.) Mol Gen Genet 204:204–207CrossRefGoogle Scholar
  232. Vasil V, Wan D, Vasil IK (1983) Plant regeneration from protoplasts of napier grass (Pennisetum purpureum Schum). Z Pflanzenphysiol 111:233–239Google Scholar
  233. Vasil IK, Srinivasa C, Vasil V (1986) Culture of protoplasts isolated from embryogenic cell suspension culture of sugarcane and maize. In: Abstr 6th Int Congr Plant tissue cell culture, Aug 3–8, 1986, Univ Minn. Minneapolis, p 443Google Scholar
  234. Wakasa K (1982) Application of tissue culture to plant breeding method — improvement and mutant production. Bull Natl Inst Agric Sci Ser D 33:121–200Google Scholar
  235. Wakasa K (1985) Selection of nitrate reductase-deficient rice cell line by calli trom microspores. Piant Tissue Cult Lett 2:33–34CrossRefGoogle Scholar
  236. Wakasa K, Watanabe Y (1979) Haploid plant of Oryza perennis (spontanea type) induced by anther culture. Jpn Breed 29:146–150Google Scholar
  237. Wakasa K, Widholm J M(1983) In vitro selection of mutant. 4, Some characters of 5-MT-resistant mutant in rice. Jpn J Breed 33(Suppl):82–83Google Scholar
  238. Wakasa K, Widholm JM (1986) 5-methyltryptophan resistant rice mutant selected in tissue culture. In: Abstr 6th Int Congr Plant tissue cell culture, Aug 3–8, 1986, Univ Minn, Minneapolis, p 440Google Scholar
  239. Walther F, Preil W(1981) Mutants tolerant to low-temperature conditionsinduced in suspension culture as a source for improvement of Euphoribia pulcherrima W illd ex Klotzsch. In: IAEA (ed) Induced mutations — a tool in plant breeding. Int Atom Energ Ag. Vienna, pp 399–405Google Scholar
  240. Wang G-Y, Hsia Z-A (1986) Protoplast culture of rice. In: Abstr 3rd Natl Meet Chin Soc Cell biology, Oct 30-Nov 4, 1986, Chengdu, China, pp 140–141Google Scholar
  241. Wang M-S, Zapata FJ, De Castro DC (1987) Plant regeneration through somatic embryogenesis from mature seed and young inflorescence ofwild rice (Oryza perennis Moench). Plant Cell Rep 6:294–296CrossRefGoogle Scholar
  242. Wang R-F, Zuo Q-X, Zheng S-W, Tian W-Z (1979) Induction of plantlets from isolated pollen culture in rice (Oryza sativa subsp, Keng). Acta Genet Sin 6:7Google Scholar
  243. Wang Y-C, Klein TM, Fromm M, Cao J, Sanford JC, Wu R (1988) Transient expression of foreign genes in rice, wheat and soybean cells following particle bombardment. Plant Molecular Biology 11:433–439CrossRefGoogle Scholar
  244. Wernicke W, Brettell R, Wakizuka T, Potrykus I (1981) Adventitious embryoid and root formation from rice leaves. Z Pflanzenphysiol 103 :361–365Google Scholar
  245. Williams EG, Maheswaran G (1986) Somatic embryogenesis: Factors influencing coordinated behaviour of cells as an embryogenic group. Ann Bot (London) 57:443–462Google Scholar
  246. Wong C-K, Ko S-W, Woo S-C (1983) Regeneration of rice plantlets on NaCl-stressed medium by anther culture. Bot Bull Acad Sin 24:59–64Google Scholar
  247. Wong C-K, Woo S-C, Ko S-W (1986) Production of rice plantlets on NaCI-stressed medium and evaluation of their progenies. Bot Bull Acad Sin 27:11–23Google Scholar
  248. Woo S-C, Huang C-Y (1980) Anther culture of Oryza glaberrima Steud and its hybrids with O. sativa L. Bot Bull Acad Sin 21:75–79Google Scholar
  249. Woo S-C, Mok T, Huang C-Y (1978) Anther culture of Oryza sativa L. and O. perennis Moench Hybrids. Bot Bull Acad Sin 19 :171–178Google Scholar
  250. Yamada Y, Ogawa M, Yano S (1983) Tissue culture in sea water increases salt tolerance of rice plants. In: Cell and tissue culture techniques for cereal crop improvement. Science Press, Beijing; IRRI, Manila, pp 229–235Google Scholar
  251. Yamada Y, Yang Z-Q, Tang D-T (1986) Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep 5:85–88CrossRefGoogle Scholar
  252. Yan C-J, Zhao Q-H (1979) Regeneration of plantlets from calli derived from leaf sheaths and rachis-branches of rice (Oryza sativa L.) Kexue Tongbao (China) 24:943–947Google Scholar
  253. Yan C-J, Zhao Q-H (1982) Callus induction and plantlet regeneration from leaf blade of Oryza sativa L. subsp. Indica. Plant Sci Lett 25:187–192CrossRefGoogle Scholar
  254. Yang Z-Q, Shikanai, Yamada Y (1988) Asymmetric hybridization between cytoplasmic male-sterile (CMS) and fertile rice (Oryza sativa L.) protoplasts. Theor Appl Genet 76:801–808CrossRefGoogle Scholar
  255. Ye H (1984) Studies on cell suspension culture and plant regeneration in rice. Acta Bot Sin 26:52–59Google Scholar
  256. Yoboue PCN (1986) Variation in genetic expression in an androgenetic progeny of rice with a high level of homozygosity. In: IAEA (ed) Nuclear techniques and in vitro culture for plant improvement. Int Atom Energ Ag, Vienna, pp 357–361Google Scholar
  257. Yu R-Q, Qie Y-W (1985) Rice germplasm. In: Lin S (ed) Rice cultivation science in China. Agronomic Press, Beijing, pp 39–83Google Scholar
  258. Yuan L-P (1972) An introduction to the breeding of male sterile lines in rice. In: Proc 2nd Worksh Genetics, Hainan, Guandong, China, March 1972Google Scholar
  259. Yuan L-P, Virmani SS (1986) Current status of hybrid rice research and development. Pres Int Symp Hybrid rice, Oct 6–10, 1986, Changsha, Hunan, ChinaGoogle Scholar
  260. Yue S-X, Liu B-L, Chen S-B (1986) A preliminary study of test-tube fertilization in rice. Acta Bot Sin 28:46–49Google Scholar
  261. Zao D (1980) “Yuan Feng Zao”, a radiation induced mutant rice cultivar, and its performance. Appl Atom Energ Agric (4):1–6Google Scholar
  262. Zapata FJ, Torrizo LB, Aldemita RR, Novero AV, Aazaredo AM, Visperas RV, Lim MS, Moon HP, Heu MH (1986) Rice anther culture: a tool for production of cold tolerant lines. In: Rice genetics. IRRI, Manila, pp 773–780Google Scholar
  263. Zhang H-M, Yang H, Rech EL, Golda TJ, Davis AS, Mulligan BJ, Cocking EC, Davey MR (1988) Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts. Plant Cell Reports 7:379–384Google Scholar
  264. Zhang L-N, Chu Q-R (1984) Characteristic and chromosome variation of somaclones and its progeny in rice (Oryza sativa L.). Sci Agric Sin (4):14–20Google Scholar
  265. Zhang TB (1983) A study of test-tube fertilization of rice (Oryza sativa L.). Acta Bot Sin 25:187–191Google Scholar
  266. Zhang W, Wu R (1988) Efficient regeneration of transgenic plants from rice protoplasts and correctly regulated expression of the foreign gene in the plants. Theor Appl Genet 76:835–840CrossRefGoogle Scholar
  267. Zhang Z-H (1982) Application of anther culture techniques to rice breeding. In: Rice tissue culture planning. Conf, IRRI, Manila, pp 55–61Google Scholar
  268. Zhang Z-H, Yin D-C (1985) Anther culture breeding and mutation breeding. In: Lin S (ed) Rice cultivation science in China. Agriculture Press, Beijing, pp 379–418Google Scholar
  269. Zhao C-Z, Zheng K-L, Qi X-F, Sun Z-X, Fu Y-P (1981) The study on the tissue and cell culture of different types of Oryza sativa L. Acta Phytophysiol Sin 7:287–290Google Scholar
  270. Zhao C-Z, Zheng K-L, Sun Z-X, Qi X-F (1984) Somaclonal variation and rice improvement. In: Abstr Int Symp Genetic manipulation in crops, Oct 22–26, Beijing, p 57Google Scholar
  271. Zhao C-Z, Sun Z-X, Zheng K-L, Qi X-F, Zhou Z-M, Fu Y-P (1986) Research on application of tissue culture to rice breeding. In: Annual reports of the China National Rice Research Institute (CNRRI) for 1983–1985. Hangzhou, China, pp 38–40Google Scholar
  272. Zheng K-L, Castiglione S, Biasini MG, Biroli A, Morandi C, Sala F (1987) Nuclear DNA amplification in cultured cells of Oryza sativa L. Theor Appl Genet 74:65–70CrossRefGoogle Scholar
  273. Zheng K-L, Zhou Z-M, Wang G-L, Luo Y-K, Xiong Z-M (1989) Somatic cell culture of rice cultivars with different grain types: somaclonal variation in some grain and quality characters. Plant Cell, Tissue and Organ Culture 18:201–208CrossRefGoogle Scholar
  274. Zheng Z-L, Chu Q-R, Zhang C-M (1985) Pathogenecity ofculture filtrate from Pyricularia oryzaeon rice. Acta Agric Shanghai 1:85–90Google Scholar
  275. Zhou C, Yang H-Y (1980) In vitro induction of haploid plantlets from unpollinated young ovaries of Oryza sativa L. Acta Genet Sin 7:287–288Google Scholar
  276. Zhou C, Yang H-Y (1981) In vitro embryogenesis in unfertilized embryo sacs of Orvza sativa L. Acta Bot Sin 23:176–180Google Scholar
  277. Zimny J, Lorz H (1986) Plant regeneration and initiation of cell suspensions from root-tip derived callus of Oryza sativa L. (rice). Plant Cell Rep 5:89–92CrossRefGoogle Scholar
  278. Zimny J, Junker B, Lorz H (1986) Root derived in vitro cultures of rice plant regeneration from callus and cell suspension, and direct gene transfer to protoplasts. In: Abstr 6th Int Congr Plant tissue and cell culture, Aug 3–8, 1986, Univ Minn, Minneapolis, p 207Google Scholar
  279. Zou G-Z, Yei M-M, Jia Y-F (1986) Establishment of rice cell line and plantlet regeneration from single cell culture. Acta Agric Shanghai 2:1–8Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Z.-X. Sun
    • 1
  • K.-L. Zheng
    • 2
  1. 1.Plant Genetics and Breeding DepartmentChina National Rice Research InstituteHangzhouChina
  2. 2.Biotechnology DepartmentChina National Rice Research InstituteHangzhouChina

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