Flow Cytometry and Cell Sorting : Problems and Promises for Biological Ocean Science Research

  • C. M. Yentsch
  • L. Cucci
  • D. A. Phinney
Part of the Lecture Notes on Coastal and Estuarine Studies book series (COASTAL, volume 8)


With the sole exception of the measurement of phytoplankton fluorescence, biological oceanographers live within the constraint of having few methods which provide real time continuous data. Most methods are discrete measurements of the “whole” community.


Accessory Pigment Sheath Fluid Subsurface Chlorophyll Maximum Chlorophyll Fluorescence Emission Forward Angle Light Scatter 
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  1. Cucci T.L., Shumway S., Selvin R., Guillard R.R.L. and Yentsch C.M. (in prep.) Approaching suspension-feeding experimentation via flow cytometry.Google Scholar
  2. Horan P.K. and Wheeless Jr. L.L. (1977) Quantitative single cell analysis and sorting. Rapid analysis and sorting of cells is emerging as an important new technology in research and medicine. Science 198 : 149–157.PubMedCrossRefGoogle Scholar
  3. Kruth H.S. (1982) Flow cytometry: Rapid biochemical analysis of single cells. Anal Biochem 125 : 225–242.PubMedCrossRefGoogle Scholar
  4. Melamed M.R., Mullaney P.F. and Mendelson M.L. (1979) Flow cytometry and sorting. John Wiley and Sons, NY. 716 pp.Google Scholar
  5. Olson R.J., Frankel S.L., Chisholm S.W. and Shapiro H.M. (1983) An inexpensive flow cytometer for the analysis of fluorescence signals in phytoplankton:chlorophyll and DNA determinations. J exp mar Biol Ecol 68 : 129–144.CrossRefGoogle Scholar
  6. Sakshaug E. (1980) Problems in the methodology of studying phytoplankton. In : Morris, I. (ed.) The Physiological Ecology of Phytoplankton, Blackwell, London, 57–91.Google Scholar
  7. Trask B.J., van den Engh G.J. and Elgershuizen J.H.B. (1982) Analysis of phytoplankton by flow cytometry. Cytometry 2(4) : 258–264.PubMedCrossRefGoogle Scholar
  8. Yentsch C.M., Horan P.K., Muirhead K, Dortch Q. , Haugen E., Legendre L. , Murphy L.S., Perry M.J., Phinney D.A., Pomponi S.A., Spinrad R.W. , Wood M. , Yentsch CS. and Zahuranec B.J. (1983a) Flow cytometry and cell sorting: a powerful technique for analysis and sorting of aquatic particles. Limnol Oceanogr 28(6) : 1275–1280.CrossRefGoogle Scholar
  9. Yentsch CM., Mague F., Horan P.K. and Muirhead K. (1983b) Flow cytometric analysis of DNA in the dinoflagellate Gonyaulax tamarensis var. excavata. J exp mar Biol Ecol 67 : 175–183.CrossRefGoogle Scholar
  10. Yentsch CM., Cucci T.L. and Phinney D.A. (submitted) Photo adaptation of Gonyaulax tamarensis to low photon flux densities: chloroplast photomorphogenesis.Google Scholar
  11. Yentsch, CS. and Yentsch CM. (1979) Fluorescence spectral signa tures: the characterization of phytoplankton populations by the use of excitation and emission spectra. J Mar Res 37 : 471–483.Google Scholar
  12. Yentsch CM. and Yentsch CS. (in press) Emergence of optical instrumentation for measuring biological properties. In : Barnes M. (ed.) Oceanography and marine biology: an annual review.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • C. M. Yentsch
    • 1
  • L. Cucci
    • 1
  • D. A. Phinney
    • 1
  1. 1.The Jane MacIsaac Flow Cytometer/Sorter FacilityBigelow Laboratory for Ocean SciencesWest Boothbay HarborUSA

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