Methods for the Investigation of Compartmentalization and Channeling
staining, e.g., in the localization of tannins, cellulose, callose, and lignin
formation of precipitates with suitable reagents, e.g., in the localization of alkaloids with KBiI4 and essential oils, flavonoids, tannins, and other secondary products with OsO4, UO2 2+, and KMnO4
chemical modification of the compounds followed by formation of precipitates, e.g., in the localization of carbohydrates after periodic acid oxidation
reaction with peroxidase-labeled or fluorescent antibodies, e.g., in the localisation of alginic acid, or with antibodies labeled with gold granules, e.g., in the localization of mannan
reaction with gold-labeled concanavalin A, e.g., in the localization of yeast mannan and other branched polysaccharides, or reaction with wheat germ agglutinin, e.g., in the localization of chitin
labeling with radioactive isotopes and autoradiographic examination, e.g., in the localization of lignin, of cell wall polysaccharides, of cyanogenic glycosides, and of alkaloids, or X-ray microanalysis, e.g., in the localization of fucoidan
examination before and after specific enzymatic degradation or selective extraction of secondary products, e.g., in the localization of carbohydrates.
KeywordsCellulose Lignin Polysaccharide Fractionation Radioactive Isotope
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Reference for Further Reading
- Luckner, M., Diettrich, B., Lerbs, W.: Cellular compartmentation and channeling of secondary metabolism in microorganisms and higher plants. Prog. Phytochem. 6, 103–142 (1980)Google Scholar