Identification of Cultivars of Grasses and Forage Legumes by SDS-PAGE of Seed Proteins
The banding patterns produced following polyacrylamide gel electrophoresis of total seed storage proteins in the presence of sodium dodecylsulphate (SDSPAGE) have proved an effective laboratory method for distinguishing cultivars of the largely cross-fertilized pasture grasses and legumes despite their high innate genetic variability (Ferguson and Grabe 1984, 1986; Gardiner et al. 1986; Gardiner and Forde 1987, 1988 a; Clark et al. 1989). The positions of the stained bands visible in the gel following electrophoresis are determined by the molecular weight of the seed proteins or their constituent polypeptides and differences between cultivars arise because of the polymorphic nature of seed storage proteins within each species. Similar techniques have been used extensively by workers concerned with cultivar identification of cultivated inbreeding crops (Cooke 1984, 1989) but to a lesser extent for differentiating cultivars of outbreeding species (Gilliland 1989).
KeywordsSeed Protein Seed Meal Banding Profile Forage Legume Trifolium Pratense
Unable to display preview. Download preview PDF.
- Biorad (1984) Acrylamide polymerisation — a practical approach. Bulletin 1156: 1–7Google Scholar
- Clark KW, Hussain A, Bamford K, Bushuk W (1989) Identification of cultivars of Agrostis species by polyacrylamide gel electrophoresis of seed proteins. In: Takatoh H (ed) Proc 6th Int Turfgrass Research Conference. Tokyo, Japan, pp 121–125Google Scholar
- Cooke RJ (1989) The use of electrophoresis for the distinctness testing of varieties of autogamous species. Plant Varieties Seeds 2:3–13Google Scholar
- Ferguson JM, Grabe DF (1984) Separation of annual and perennial species of ryegrass by gel elctrophoresis of seed proteins. J Seed Technol 9:137–149Google Scholar
- Forde MB, Gardiner SE (1991) Electrophoretic seed protein banding patterns as genetic resource descriptors. In: Zakri AH (eds) Conservation of plant genetic resources through in vitro methods. FRIM/MNCPGR, Kuala Lumpur, pp 95–108Google Scholar
- Gardiner SE, Forde MB (1987) SDS polyacrylamide gel electrophoresis of grass seed proteins: a method for cultivar identification of pasture grasses. Seed Sci Technol 15: 663–674Google Scholar
- Gardiner SE, Forde MB (1988 a) Identification of cultivars and species of pasture legumes by sodium dodecylsulphate polyacrylamide gel electrophoresis of seed proteins. Plant Varieties Seeds 1: 13–26Google Scholar
- Gardiner SE, Forde MB (1988 b) Fingerprinting pasture species. In: Zakri AH (ed) Plant breeding and genetic engineering. SABRAO, Malaysia, pp 353–359 Identification of Cultivars of Grasses and Forage Legumes by SDS-PAGE of Seed Proteins 61Google Scholar
- Gilliland TJ (1989) Electrophoresis of sexually and vegetatively propagated cultivars of allogamous species. Plant Varieties Seeds 2: 15–25Google Scholar
- Gilliland TJ, Camlin MS, Wright CE (1982) Evaluation of phosphoglucosomerase allozyme electrophoresis for the identification and registration of cultivars of perennial ryegrass (Latium perenne) Seed Sci Technol 10: 415–430Google Scholar
- Slack CR, Hancock DA, Griffin WB, McEwan JM (1985) Separation of proteins from grain of New Zealand grown wheat and barley varieties by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE). Plant Physiology Division, DSIR, Tech Rep 21: 1–19Google Scholar
- Smith DB, Payne PI (1984) A procedure for the routine determination of electrophoretic band patterns of barley and malt endosperm proteins. J Nat Inst Agric Bot 16: 487–498Google Scholar