Seed Analysis pp 361-375 | Cite as

Analysis of Limonoids in Citrus Seeds

  • Z. Herman
  • C. H. Fong
  • S. Hasegawa
Part of the Modern Methods of Plant Analysis book series (MOLMETHPLANT, volume 14)

Abstract

Limonoids are a group of chemically related triterpene derivatives found in the Rutaceae and Meliaceae families. Among the 38 limonoids reported to occur in citrus and its hybrids, four are known to be bitter in taste. These are limonin, nomilin, ichangin, and nomilinic acid. Limonin is the primary cause of citrus juice bitterness. This problem occurs in a variety of citrus juices, and it is economically important to the citrus industry because bitter juices have a lower market value for producers. Dekker (1988) has reviewed limonoid bitterness and postharvest juice debittering methods. Hasegawa (1989) has reviewed limonoid biochemistry and preharvest approaches to the bitterness problem.

Keywords

Hexane Fractionation Flavonoid Glycoside Aspergillus 

Abbreviations Used

CH2C12

Dichlormethane

CH3CN

Acetonitrile

C6H6

Benzene

H2O

Water

EtOAc

Ethyl acetate

Me2CO

Acetone

MeOH

Methanol

NH4C1

Ammonium chloride

HAc

Acetic acid

H2SO4

Sulfuric acid

HC1

Hydrogen chloride

KHCO3

Postassium bicarbonate

H3PO4

Phosphoric acid

CHC13

Chloroform

Rf

Ratio of the distance traveled by a compound divided by the distance traveled by the solvent front (used in TLC)

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • Z. Herman
  • C. H. Fong
  • S. Hasegawa

There are no affiliations available

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