Seed Analysis pp 241-261 | Cite as

Glycolipid Analysis in Wheat Grains

  • N. G. Larsen
Part of the Modern Methods of Plant Analysis book series (MOLMETHPLANT, volume 14)


Cereal lipids are broadly classified according to the grain tissues (germ, aleurone, endosperm) from which they are extracted and, like proteins, their extraction behaviour in solvents. For example, endosperm glycolipids can be extracted from outside (“non-starch” glycolipids) or inside (“starch” glycolipids) the starch granules (Chung 1986).


High Performance Liquid Chromatography Wheat Flour Silicic Acid Free Lipid Flour Lipid 
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  1. Barnes PJ (1987) Wheat grain lipids and their role in the bread-making process. In: Hamilton RJ, Bhati A (eds) Recent advances in chemistry and technology of fats and oils. Elsevier, London, pp 79–107CrossRefGoogle Scholar
  2. Bekes F, Zawistowska U, Bushuk W (1983) Protein — lipid complexes in the gliadin fraction. Cereal Chem 60: 371–378Google Scholar
  3. Bekes F, Zawistowska U, Zillman RR, Bushuk W (1986) Relationship between lipid content and composition and loaf volume of twenty six common spring wheats. Cereal Chem 63:327— 331Google Scholar
  4. Bell BM, Daniels DGH, Stewart BA (1987) Lipid compositions, baking qualities and other characteristics of wheat varieties grown in the UK. J Cereal Sci 5: 277–286CrossRefGoogle Scholar
  5. Berger M (1983) Composition lipidique de huit farines de blé tendre. Ind des Céréales 22: 5–26Google Scholar
  6. Blunt JW, Munro MHG (1976) An automated procedure for qualitative and quantitative analysis of mixtures by means of carbon magnetic resonance spectroscopy: applications to carbohydrate analysis. Aust J Chem 29: 975–986CrossRefGoogle Scholar
  7. Carter HE, McCluer RH, Slifer ED (1956) Lipids of wheat flour. I. Characterization of galactosylglycerol components. J Am Chem Soc 78: 3735–3738Google Scholar
  8. Carter HE, Ohno K, Nojima S, Tipton CL, Stanacev NZ (1961 a) Wheat flour lipids. II. Isolation and characterization of glycolipids of wheat flour and other plant sources. J Lipid Res 2:215 — 222Google Scholar
  9. Carter HE, Hendry RA, Stanacev NZ ( 1961 b) Wheat flour lipids. III. Structure of the mono-and digalactosylglycerol lipids. J Lipid Res 2: 223–227Google Scholar
  10. Christie WW (1982) Lipid analysis, 2nd edn. Pergamon Press, OxfordGoogle Scholar
  11. Christie WW (1985) Rapid separation and quantification of lipid classes by high performance liquid chromatography and mass (light-scattering) detection. J Lipid Res 26: 507–512PubMedGoogle Scholar
  12. Christie WW (1987) High performance liquid chromatography and lipids. A practical guide. Pergamon Press, OxfordGoogle Scholar
  13. Christie WW (1989) Gas chromatography and lipids: a practical guide. Oily Press, Ayr, Scotland Christie WW, Morrison WR (1988) Separation of complex lipids of cereals by high-performance liquid chromatography with mass detection. J Chromatogr 436: 510–513Google Scholar
  14. Chung OK (1986) Lipid-protein interactions in wheat flour, dough, gluten, and protein fractions. Cereal Foods World 31: 242–256Google Scholar
  15. Chung OK (1989) Functional significance of wheat lipids. In: Pomeranz Y (ed) Wheat is unique. AACC, St Paul, MN, pp 341–368Google Scholar
  16. Chung OK, Pomeranz Y, Finney KF, Hubbard JD, Shogren MD (1977) Defatted and reconstituted wheat flours. I. Effects of solvent and Soxhlet types on functional (breadmaking) properties. Cereal Chem 54: 454–465Google Scholar
  17. Chung OK, Pomeranz Y, Jacobs RM, Howard BG (1980) Lipid extraction conditions to differenti ate among hard red winter wheats that vary in breadmaking. J Food Sci 45: 1168–1174CrossRefGoogle Scholar
  18. Chung OK, Pomeranz Y, Finney KF (1982) Relation of polar lipid content to mixing requirement and loaf volume potential of hard red winter wheat flour. Cereal Chem 59: 14–20Google Scholar
  19. Chung OK, Pomeranz Y, Jacobs RM (1984) Solvent solubility parameter and flour moisture effects on lipid extractibility. J Am Oil Chem Soc 61: 793–797CrossRefGoogle Scholar
  20. Clements RL (1977) Large scale laboratory Soxhlet extraction of wheat flours, and of intact and cracked grains. Cereal Chem 54: 865–874Google Scholar
  21. Clements RL, Donelson JR (1981) Functionality of specific flour lipids in cookies. Cereal Chem 58: 204–206Google Scholar
  22. Daftary RD, Pomeranz Y, Shogren M, Finney KF (1968) Functional breadmaking properties of wheat flour lipids. 2. The role of flour lipid fractions in breadmaking. Food Technol 22:327 — 330Google Scholar
  23. Daniels NWR, Richmond JW, Eggitt PWR, Coppock JBM (1966) Studies on the lipids of flour. III. Lipid binding in breadmaking. J Sci Food Agric 17: 20–29Google Scholar
  24. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350–356CrossRefGoogle Scholar
  25. Fujino Y, Ohnishi M (1983) Sphingolipids in wheat grain. J Cereal Sci 1: 159–168CrossRefGoogle Scholar
  26. Gordon AJ, Ford RA (1972) The chemists companion. A handbook of practical data, techniques, and, references. John Wiley & Sons, New York, p 379Google Scholar
  27. Graveland A (1968) Combination of thin layer chromatography and gas chromatography in the analysis on a microgram scale of lipids from wheat flour and wheat flour doughs. J Am Oil Chem Soc 45: 834–840PubMedCrossRefGoogle Scholar
  28. Hamilton RJ, Mitchell SF, Sewell PA (1987) Techniques for the detection of lipids in high performance liquid chromatography. J Chromatogr 395: 33–46PubMedCrossRefGoogle Scholar
  29. Hammam H, Sivik B, Schwengers D (1987) Baking qualities of CO2- and ethanol-extracted gluten and gluten lipids. Acta Agric Scand 37: 130–136CrossRefGoogle Scholar
  30. Hargin KD, Morrison WR (1980) The distribution of acyl lipids in the germ aleurone, starch and non-starch endosperm of four wheat varities. J Sci Food Agric 31: 877–888CrossRefGoogle Scholar
  31. Heemskerk JWM, Bögemann G, Scheijen MAM, Wintermans JFGM (1986) Separation of chloroplast polar lipids and measurement of galactolipid metabolism by high-performance liquid chromatography. Anal Biochem 154: 85–91PubMedCrossRefGoogle Scholar
  32. Hoseney RC, Finney KF, Pomeranz Y, Shogren MD (1969) Functional (breadmaking) and biochemical properties of wheat flour components. V. Role of total extractable lipids. Cereal Chem 46: 606–613Google Scholar
  33. Kates M (1964) Simplified procedures for hydrolysis or methanolysis of lipids. J Lipid Res 5: 132–135PubMedGoogle Scholar
  34. Kushwaha SC, Kates M (1981) Modification of phenol-sulphuric acid method for estimation of sugars in lipids. Lipids 16: 372–373PubMedCrossRefGoogle Scholar
  35. Larsen NG, Baruch DW, Humphrey-Taylor VJ (1986) Lipid quality factors in breeding New Zealand wheats. In: Williams TA, Wratt GS (eds) Plant Breeding Symp DSIR, Agronomy Society of New Zealand Spec Publ 5. Agron Soc of NZ, Christchurch, pp 278–281Google Scholar
  36. Larsen NG, Baruch DW, Humphrey-Taylor VJ (1989a) The effect of laboratory and commercial milling on lipids and other physicochemical factors affecting the breadmaking quality of wheat flour. J Cereal Sci 9: 139–148CrossRefGoogle Scholar
  37. Larsen NG, Humphrey-Taylor VJ, Baruch DW (1989b) Glycolipid content as a breadmaking quality determinant in flours from New Zealand wheat blends. J Cereal Sci 9: 149–157CrossRefGoogle Scholar
  38. Larsen NG, Levick SM, Mouat CH, Sutton KH (1990) The effect of ball-milling on phospholipid extractability and the breadmaking quality of flour. J Cereal Sci 12:155 —164Google Scholar
  39. Larsson K (1983) Physical state of lipids and their technical effects in baking. In: Barnes PJ (ed) Lipids in cereal technology. Academic Press, London, pp 237–251CrossRefGoogle Scholar
  40. Lin MJY, Youngs VL, D’Appolonia BL (1974a) Hard red spring and durum wheat polar lipids. I. Isolation and quantitative determinations. Cereal Chem 51: 17–33Google Scholar
  41. Lin MJY, D’Appolonia BL, Youngs VL (1974b) Hard red spring and durum wheat polar lipids. II. Effect on quality of bread and pasta products. Cereal Chem 51: 34–45Google Scholar
  42. Lundberg WO (1984) Lipidology. In: Mangold HK (ed) CRC handbook of chromatography. Lipids, vol I. CRC Press, Boca Raton, p 23Google Scholar
  43. MacRitchie F (1977) Flour lipids and their effects in baking. J Sci Food Agric 28:53–58 MacRitchie F (1983) Role of lipids in baking. In: Barnes PJ (ed) Lipids in cereal technology. Academic Press, London, pp 165–188Google Scholar
  44. Marion D, Le Roux C, Akoka S, Tellier C, Gallant D (1987) Lipid-protein interactions in wheat gluten: a phosphorus nuclear magnetic resonance spectroscopy and freeze-fracture electron microscopy study. J Cereal Sci 5: 101–115CrossRefGoogle Scholar
  45. McKillican ME (1964) Studies on the phospholipids, glycolipids and sterols of wheat endosperm. J Am Oil Chem Soc 41: 554–557CrossRefGoogle Scholar
  46. McKillican ME, Sims RPA (1964) The endosperm lipids of three Canadian wheats. J Am Oil Chem Soc 41: 340–344CrossRefGoogle Scholar
  47. Morrison WR (1978) Cereal lipids. In: Pomeranz Y (ed) Advances in cereal science and technology, vol II. AACC, St Paul, MN, pp 221 — 348Google Scholar
  48. Morrison WR (1983) Acyl lipids in cereals. In: Barnes PJ (ed) Lipids in cereal technology. Academic Press, London, pp 11–32CrossRefGoogle Scholar
  49. Morrison WR (1988a) Lipids. In: Pomeranz Y (ed) Wheat chemistry and technology, vol 1, 3rd edn. AACC, St Paul, MN, pp 373–439Google Scholar
  50. Morrison WR (1988b) Lipids in cereal starches. A review. J Cereal Sci 8: 1–15CrossRefGoogle Scholar
  51. Morrison WR (1989) Wheat lipids are unique. In: Pomeranz Y (ed) Wheat is unique. AACC, St Paul, MN, pp 319–339Google Scholar
  52. Morrison WR, Mann DL, Soon W, Coventry AM (1975) Selective extraction and quantitative analysis of non-starch and starch lipids from wheat flour. J Sci Food Agric 26: 507–521PubMedCrossRefGoogle Scholar
  53. Morrison WR, Tan SW, Hargin KD (1980) Methods for the quantitative analysis of lipids in cereal grains and similar tissues. J Sci Food Agric 31: 329–340PubMedCrossRefGoogle Scholar
  54. Morrison WR, Coventry AM, Barnes PJ (1982) The distribution of acyl lipids and tocopherols in flours millstreams. J Sci Food Agric 33: 925–933CrossRefGoogle Scholar
  55. Morrison WR, Wylie LJ, Law CN (1984) The effect of Group 5 chromosomes on the free and total galactosyldiglycerides in wheat endosperm. J Cereal Sci 2: 145–152CrossRefGoogle Scholar
  56. Morrison WR, Law CN, Wylie LJ, Coventry AM, Seekings J (1989) The effect of Group 5 chromosomes on the free polar lipids and breadmaking quality of wheat. J Cereal Sci 9: 41–51CrossRefGoogle Scholar
  57. Panozzo JF, O’Brien L, MacRitchie F, Bekes F (1990) Baking quality of Australian wheat cultivars varying in their free lipid composition. J Cereal Sci 11: 51–57CrossRefGoogle Scholar
  58. Pomeranz Y (1965) Thin layer chromatography in studies of cereal lipids. Qual Plant Mater Veg XII:321 — 341Google Scholar
  59. Pomeranz Y (1980) What? How much? Where? What function? in bread making. Cereal Foods World 25: 656–662Google Scholar
  60. Pomeranz Y (1988) Composition and functionality of wheat flour components. In: Pomeranz Y (ed) Wheat chemistry and technology, vol II, 3rd edn. AACC, St Paul, MN, pp 285–328Google Scholar
  61. Pomeranz Y, Chung O, Robinson RJ (1966) The lipid composition of wheat flours varying widely in bread-making potentialities. J Am Oil Chem Soc 43: 45–48PubMedCrossRefGoogle Scholar
  62. Scott TA, Melvin EH (1953) Determination of dextran with anthrone. Anal Chem 25:1656–1661 Simmons CT (1986) The development and application of new infrared methods for determining the glycolipid fraction in hard red spring wheats. Diss Abstr Int 47 (1): 20Google Scholar
  63. Simmons CT, McDonald CE (1985) A near infrared reflectance method for the glycolipid fraction in wheat flour. Cereal Foods World 30: 566Google Scholar
  64. Tweeten TN, Wetzel DL, Chung OK (1981) Physicochemical characterization of galactosyldiglycerides and their quantitation in wheat flour lipids by high performance liquid chromatography. J Am Oil Chem Soc 58: 664–672CrossRefGoogle Scholar
  65. Walker GC (1988) Determination of flour glycolipids as their benzoyl derivatives by high-performance liquid chromatography with ultraviolet detection. Cereal Chem 65: 433–435Google Scholar
  66. Williams JP, Watson GR, Khan M, Leung S, Kukis A, Stachnyk O, Myhler JJ (1975) Gas-liquid chromatography of plant galactolipids and their deacylation and methanolysis products. Anal Biochem 66: 110–122PubMedCrossRefGoogle Scholar
  67. Yamamoto A, Rouser G (1970) Spectrophotometric determination of molar amounts of glycosphingolipids and ceramide by hydrolysis and reaction with trinitrobenzenesulfonic acid. Lipids 5: 442–444PubMedCrossRefGoogle Scholar
  68. Youngs VL, Medcalf DG, Gilles KA (1970) The distribution of lipids in fractionated wheat flour. Cereal Chem 47: 640–649Google Scholar
  69. Yu RK, Koerner TAW, Scarsdale JN, Prestegard JH (1986) Elucidation of glycolipid structure by protein nuclear magnetic resonance spectroscopy. Chem Phys Lipids 42: 27–48PubMedCrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1992

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  • N. G. Larsen

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