Three-dimensional organization of actin cytoskeleton and podosomal contact structures in neoplastic cells in vitro
In spontaneously metastasizing rat RPS sarcoma cells, a 3D structure of oblique F-actin cables was observed which was associated with active cell migration in vitro. This led us to further comparative investigations of several other neoplastic and normal cell populations in vitro for F-actin structures using confocal laser scanning microscopy (CLSM). Various forms of F-actin cytoskeleton were observed and the incidence of podosome-related contact structures appeared to be associated with malignancy, interpreted as metastatic capacity.
Key wordsActin cytoskeleton Neoplastic cells Podosomal contact structures
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