Abstract
Ex vivo perfusions of human decay accelerating factor-expressing transgenic (n = 3), and nontransgenic (n = 6) porcine livers with human blood revealed a higher degree of organ damage in nontransgenic pig livers. Transgenic livers were protected from immunohistologically detectable complement deposition, despite corresponding IgM and IgG deposits in both groups. Complement activation and consumption of C3 and C4 turned out to be lower in transgenic pig livers. In contrast to livers of normal landrace pigs, livers from genetically manipulated pigs showed no morphological alterations after perfusion.
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References
Baldwin WM III, Pruitt SK, Brauer RB, et al (1995) Complement in organ transplantation — contributions to inflammation, injury and rejection. Transplantation 59: 797–808
Burdick JF, Fair JH (1994) Xenoperfusion: the pig liver as a bridge. Xeno 2: 3–5
Carrington CA, Richards AC, Cozzi E, et al (1995) Expression of human DAF and MCP on pig endothelial cells protects from human complement. Transplant Proc 27: 321–323
Chari RS, Collins BH, Magee JC, et al (1994) Treatment of hepatic failure with ex vivo pig liver perfusion followed by liver transplantation. N Engl J Med 331: 234–237
Cozzi E, Langford GA, Wright L, et al (1995) Comparative analysis of human DAF expression in the tissues of trans-genic pigs and man. Transplant Proc 27: 319–320
Makowka L, Cramer DV, Hoffman A, et al (1995) The use of a pig liver xenograft for temporary support of a patient with fulminant hepatic failure. Transplantation 59: 1654–1659
McKenzie IFC, Koulmanda M, Mandel T, et al (1995) Comparative studies of the major xenoantigen gala(1,3)gal in pigs and mice. Transplant Proc 27: 247–248
Neuhaus P, Blumhardt G (1993) Extracorporeal liver perfusion: applications of an improved model for experimental studies of the liver. Int J Artif Organs 16: 729–739
Rosengard AM, Cary NRB, Langford GA, et al (1995) Tissue expression of human complement inhibitor, decay accelerating factor, in transgenic pigs — a potential approach for preventing xenograft rejection. Transplantation 59: 1325–1333
Schön MR, Lemmens HP, Neuhaus P, et al (1994) Improved xenogeneic extracorporeal liver perfusion. Transplant Proc 26: 1293–1297
Yannoutsos N, Langford GA, Cozzi E et al (1995) Production of pigs trans-genic for human regulators of complement activation. Transplant Proc 27: 324–325
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© 1996 Springer-Verlag Berlin Heidelberg
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Pascher, A. et al. (1996). Expression of human decay accelerating factor (hDAF) in transgenic pigs regulates complement activation during ex vivo liver perfusion — immunopathological findings. In: Mühlbacher, F., et al. Transplant International . Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-00818-8_93
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DOI: https://doi.org/10.1007/978-3-662-00818-8_93
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-61024-3
Online ISBN: 978-3-662-00818-8
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