Prolonged allograft survival by the inhibition of costimulatory CD2 signals but not by modulation of CD48 (CD2 ligand) in the rat
The CD2 receptor is an important costimulatory molecule in T cell activation. Its ligand CD48 in rodents is supposed to be a homologue of human CD58, because of its similarities in structure and distribution. We evaluated the immunosuppressive activity of CD2/CD48-directed therapy in vitro and in vivo for the efficacy in prolonging rat heart allograft survival in a high responder transplant model. CD2-directed monoclonal antibody (mAb) therapy significantly prolonged median survival time to 45 days (P < 0.001). Suppression was mediated by down-modulation of CD2 below 20% on lymph node cells without considerable cell depletion. In contrast, CD48 mAb could not prolong graft survival. Rejection occurred in the presence of complete CD48 modulation and, therefore, despite disruption of the CD2–CD48 interaction. CD48 mAb failed to inhibit lymphocyte activation via a mitogenic pair of CD2 mAbs and inhibited mixed lymphocyte reaction (MLR) only by an unspecific mechanism. In conclusion, our results suggest a negative regulatory signal transduction by inhibitory CD2 mAbs and argue against a pivotal role of mere disruption of the CD2–CD48 interaction in CD2-mediated immunosuppression.
Key wordsTransplantation Graft survival Antibody therapy Costimulatory activation signals Receptor-ligand binding
Unable to display preview. Download preview PDF.
- 1.Arvieux J, Jefferies WA, Paterson DJ, Williams AF, Green JR (1986) Monoclonal antibodies against a rat leukocyte antigen block antigen-induced T-cell responses via an effect on accessory cells. Immunol 58: 337–342Google Scholar
- 12.Merwe A van der, Brown PA, Davis MH, Barclay SJ (1993) Affinity and ki netic analysis of the interaction of the cell adhesion molecules rat CD2 and CD48. EMBO J 12: 4945–4954Google Scholar