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Chronic rejection in lung allografts: immunohistological analysis of fibrogenesis

  • Takeshi Hirabayashi
  • S. Demertzis
  • J. Schäfers
  • Ken Hoshino
  • Björn Nashan

Abstract

In ongoing chronic rejection after lung transplantation, alveolar interstitial fibrosis develops. However, little is known about the mechanisms involved. In order to investigate these mechanisms, expression of extracellular matrix molecules (ECM) (undulin, decorin, tenascin, laminin, and fibronectin) and cytokines [transforming growth factor(TGF)-βl, TGF-β3, platelet-derived growth factor(PDGF), and PDGF receptor] were semiquantitatively evaluated in chronically rejected lung allografts, using standard immunohistochemical techniques. Additionally, the presence of macrophages was analysed. The present study demonstrates an increased infiltration of macrophages with a concomitant upregulation of cytokines (TGF-β1, TGF-β3, and PDGF) and an increased deposition of ECM in chronic lung rejection. These cytokines have an important role in the stimulation of fibroblasts which are a major source of ECM. Upregulated expression of ECM in the alveolar interstitial space leads to alveolar malfunction by thickening of the wall and, thus, is one of the causative factors of respiratory dysfunction in chronic lung graft rejection.

Key words

Chronic rejection Alveolar interstitial fibrosis Extracellular matrix TGF-β PDGF 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Takeshi Hirabayashi
    • 1
  • S. Demertzis
    • 2
  • J. Schäfers
    • 2
  • Ken Hoshino
    • 3
  • Björn Nashan
    • 1
  1. 1.Klinik für Abdominal- und TransplantationschirurgieMedizinische Hochschule HannoverHannoverGermany
  2. 2.Klinik für Herz-, Thorax-, GefässchirurgieMedizinische Hochschule HannoverHannoverGermany
  3. 3.Department of Surgery, School of MedicineKeio UniversityShinjuku, 160, TokyoJapan

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