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Liposoluble antioxidants are not consumed in the pancreas after reperfusion in human simultaneous pancreas-kidney transplantation

  • S. Benz
  • F. Pfeffer
  • M. Büsing
  • M. R. Clemens
  • A. R. Waladkhani
  • H. D. Becker
  • U. T. Hopt
Conference paper

Abstract

There is considerable evidence that under experimental conditions, oxygen free radicals (OFRs) are decisive in the pathogenesis of ischemia-reperfusion injury. Normally OFRs are scavenged by antioxidants such as α-tocopherol. Thus, in the following study we investigated whether antioxidants are consumed locally in human pancreatic grafts after reperfusion. A series of ten patients receiving bladderdrained pancreaticoduodenal and renal allografts were studied. Sequential blood samples were drawn locally from the venous outflow of the graft and simultaneously from the radial artery after reperfusion. α-Tocopherol, retinol, lycopene, and α-and ß -carotene levels were determined. After reperfusion these levels remained largely unchanged. Hence, in our study a consumption of antioxidants locally in the pancreatic graft after transplantation was not demonstrated. Therefore, the antioxidant capacity seems not to be exhausted at that time. This suggests that in clinical pancreatic transplantation oxidant stress in the initial reperfusion period might not have the relevance suggested by animal models.

Key words

Antioxidants Tocopherol Graft pancreatitis Oxygen free radicals Pancreatic transplantation 

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References

  1. 1.
    Abe K, Yoshida S, Watson B, Busto R, Kogure K, Ginsberg MD (1983) Tocopherol and ubichinone in rat brain subjected to decapitation ischemia. Brain Res 273: 166–169PubMedCrossRefGoogle Scholar
  2. 2.
    Abendroth D, Schneeberger S, Schleibner S, Illner WD, Land W (1992) Stellenwert der Behandlung mit Radikalfängern nach Nieren-und Pankreas-transplantation Zentralbl Chir 117: 502–508Google Scholar
  3. 3.
    Burton GW, Joyce A, Ingold KU (1983) Is vitamin E the only lipisoluble chain breaking antioxidant in human blood plasma and erythrocytes membranes? Arch Biochem Biophys 221: 1–10Google Scholar
  4. 4.
    Granger N (1988) The role of xanthine oxidase and granulocytes in ischemia — and reperfusion injury. Am J Physiol 255: 1269–1275Google Scholar
  5. 5.
    Hopt UT, Busing M, Schareck WD, Becker HD (1992) Management der exocrinen Pankreasekretion. Ein zentrales Problem der allogenen Pankreas-transplantation. Chirurg 63: 186–192Google Scholar
  6. 6.
    Jaeschke H (1991) Vascular oxidant stress and hepatic ischemia/reperfusion injury Free Radic Res Commun 12: 737–743CrossRefGoogle Scholar
  7. 7.
    Jaeschke HJ (1991) Reactive oxygen and ischemia/reperfusion injury in the rat liver Chem Biol Interact 79: 115–133CrossRefGoogle Scholar
  8. 8.
    Jaeschke H, Farhood A, Smith W (1990) Neutrophils contribute to ischemia/reperfusion injury in rat liver in vivo. FASEB J 4: 3355–3359PubMedGoogle Scholar
  9. 9.
    Jaeschke H, Farhood A, Bautista P, Zoltan S, Spitzer J, Smith W (1993) Functional inactivation of neutrophils with a Mac-1 (CD11b/CD18) mono-clonal antibody protects against is-chemia- and reperfusion injury in rat liver. Hepatology 17: 915–923PubMedCrossRefGoogle Scholar
  10. 10.
    Murphy ME, Kolvenbach R, Aleksis M, Hansen R, Sies H (1992) Antioxidant depletion in aortic crossclamping is-chemia: increase in the plasma level of tocopherol quinone/tocopherol ratio.Free Radic Bio Med 13: 95–100Google Scholar
  11. 11.
    Oda T, Nakai I, Mituo H, Oka T,Yoshikawa T (1992) Role of oxygen free radicals and the synergistic effect of dismutase and catalase on ischemiareperfusion injury of the rat pancreas. Transplant Proc 24: 797–798Google Scholar
  12. 12.
    Pincemail J, Defraigne JO, Franssen C, Bonnet P, Deby-Dupont G, Pirenne J, Deby C, Lamy M, Limet M, Meurisse M (1993) Evidence for free radical formation during human kindey transplantation. Free Radic Bio Med 15: 343–348CrossRefGoogle Scholar
  13. 13.
    Sanfey H, Sarr M, Bulkley G, Cameron J (1986) Oxygen-free radicals and acute pancreatitis. Acta Physiol Scand [Suppl] 548: 109–118Google Scholar
  14. 14.
    Serino F, Citterio F, Lippa S, Oradei A, Agnes S, Nanni G, Pozzetto A, Littarru G, Castagneto M (1990) Coenzyme Q, alpha-tocopherol, and delayed function in human kidney transplantation.Transplant Proc 22: 1375–1378Google Scholar
  15. 15.
    Tamura K, Manabe T, Kyogoku T, Andoh K, Ohshio G, Tobe T (1993) Effect of postischemic reperfusion on the pancreas. Hepatogastroenterology 40: 452–454PubMedGoogle Scholar
  16. 16.
    Welbourn C, Goldmann G, Paterson I, Valeri CR, Shepro D, Hechtmann HB (1991) Pathophysiology of ischemia and reperfusion injury: central role of the neutrophil. Br J Surg 78: 651–655PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • S. Benz
    • 1
  • F. Pfeffer
    • 1
  • M. Büsing
    • 4
  • M. R. Clemens
    • 2
  • A. R. Waladkhani
    • 2
  • H. D. Becker
    • 3
  • U. T. Hopt
    • 1
  1. 1.Chirurgische Universitätsklinik RostockRostockGermany
  2. 2.Medizinische Klinik der Universität TübingenTübingenGermany
  3. 3.Chirurgischen Universitätsklinik TübingenTübingenGermany
  4. 4.Chirurgische Klinik, KnappschaftskrankenhausBochum-Langendreer-UniversitätsklinikIn der Schornau, BochumGermany

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