Assessment of oxygen radicals during kidney transplantation — effect of radical scavenger
In the present study, levels of free oxygen radicals, generated in the very early period of reperfusion during human kidney transplantation, were assessed by determination of malondialdehyde (MDA) levels using a high-pressure liquid chromatography (HPLC) method. Renal blood samples were obtained during reperfusion by intraoperative cannulation of the renal vein. Simultaneously, systemic MDA levels were determined Furthermore, local and systemic levels of interleukin 6 (IL-6), tumor necrosis factor (TNF) receptors, p55 and p75, and vitamin E were measured. In a second group of patients, 500 mg of ascorbic acid were given prior to reperfusion. Renal MDA levels in the control group were always higher compared to systemic levels. IL-6 showed a marked increase shortly after reperfusion in the renal blood. In the scavenger group there was a diminution of these effects. TNF receptor levels and vitamin E remained largely unchanged. The results of this pilot study demonstrated clinically the moderate production of reactive oxygen species and the liberation of IL-6 shortly after reperfusion of human transplanted kidneys. Furthermore, the modulating effect of a radical scavenger on these effects was shown.
Key wordsOxygen free radicals Kidney transplantation IL-6 TNF receptors Vitamin E Radical scavenger
Unable to display preview. Download preview PDF.
- 2.Schneeberger H, Schleibner S, Illner WD, Messmer K, Land W (1993) The impact of free radical-mediated reperfusion injury on acute and chronic rejection events following cadaveric renal transplantation. Clin Transplant 219–232Google Scholar
- 5.Land W, Schneeberger H, Schleibner S, Illner WD, Abendroth D, Rutili G, Ar-fors KE, Messmer K (1994) The beneficial effect of human recombinant superoxide dismutase on acute and chronic rejection events in recipients of cadaveric renal transplants. Transplantation 57: 211–217PubMedCrossRefGoogle Scholar
- 7.Parks DA, Bulkley GB, Granger DN (1983) Role of oxygen free radicals in shock, ischemia, and organ preservation. Surgery 94: 407–411Google Scholar
- 8.Granger DN, Höllwarth ME, Parks DA (1986) Ischemia-reperfusion injury: role of oxygen derived free radicals. Acta Physiol Scand 548: 47–63Google Scholar
- 11.Bittermann H, Lazarovich H, Kinarty A, Cohen L, Lahat N (1990) The profile of cytokines and cytokine inhibition during splanchnic artery occlussion ( SAO) shock. Circ Shock 31: 25Google Scholar
- 12.Defraigne JO, Pincemail J, Franssen C, Meurisse D, Defechereux T, Philippart C, Serteyn D, Lamy M, Deby C, Limet R (1993) In vivo free radical production after cross-clamping and reperfusion of the renal artery in the rabbit. Cardiovase Surg 1: 343–349Google Scholar