Competitive Protein-Binding Assay for the Diagnosis of Hyper- and Hypovitaminosis D

  • V. Bothe
  • H. Schmidt-Gayk


Vitamin D is absorbed from the diet and produced in the skin by UV light radiation. It is then metabolized by the liver to a more potent compound, 25-hydroxyvitamin D (25-OH-D), which is the major metabolite in plasma. A large number of binding assays for 25-OH-D have been published, as shown in Table 1. Using the competitive binding assay described by Belsey et al. (1974), a shortcoming in the assay was observed to be insufficient binding of [3H]25-OH-D to the binding protein, with fairly high coefficients of variation. Therefore, the conditions of the assay were reevaluated. As will be shown, the result was an assay entirely different from the published ones. The extraction, binding protein, gelatin concentration, and tracer solvent differ from those in the published methods. In addition, this assay circumvents the need for evaporation of the extract.


Nephrotic Syndrome Ethanol Extraction Femoral Neck Fracture Continuous Ambulatory Peritoneal Dialysis Competitive Binding Assay 
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© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • V. Bothe
  • H. Schmidt-Gayk

There are no affiliations available

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