Skip to main content
SpringerLink
Log in

Sensitive Adenylate Cyclase Bioassay for Parathyroid Hormone

  • Chapter
Calcium Regulating Hormones, Vitamin D Metabolites, and Cyclic AMP Assays and Their Clinical Application

  • 96 Accesses

Abstract

A large number of substances such as dopamine, secretin, prostaglandin E2, βadrenergic catechecholamines, and parathyroid hormone (PTH) activate adenylate cyclase by interacting with appropriate endogenous ligands on the cell surface. These interactions ultimately result in stimulation of adenylate cyclase activity and thus in an increase of cAMP production. Prostaglandin F2 and α2-adrenergic substances, however, lower the cAMP level by inhibiting adenylate cyclase.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Gilman AG (1984) G proteins and dual control of adenvlate cvclase. Cell 36:577–579

    Article  PubMed  CAS  Google Scholar 

  2. Niepel B, Radeke H, Atkinson MJ, Jueppner H, Hesch RD (1983) A homologous biological probe for parathyroid hormone in human serum. J Immunoassay 4:21–47

    Article  PubMed  CAS  Google Scholar 

  3. Katada T, Ui M (1982) ADP ribosylation of the specific membrane protein C 6 cells by islet activating protein associated with modification of adenylate cyclase activity. J Biol Chem; 257:7210–7216

    PubMed  CAS  Google Scholar 

  4. Tomlinson S, Mac Neil S, Brown BL (1985) Calcium, cyclic AMP, and hormone action. Clin Endocrinol (Oxf) 23:595–610

    Article  CAS  Google Scholar 

  5. Cohen P (1985) The role of protein phosphorylation in hormonal control of enzyme activity. Eur J Biochem 154:439–448

    Article  Google Scholar 

  6. Chambers DJ, Dunham J, Zanelli JM, Parsons JA, Bitensky L, Chayen J (1978) A sensitive bioassay of parathyroid hormone in plasma. Clin Endocrinol (Oxf) 9:375–379

    Article  CAS  Google Scholar 

  7. Chase LR, Aurbach GD (1967) Parathyroid function and the renal excretion of 3′,5′-adenylic acid. Proc Natl Acad Sci USA 58:518–525

    Article  PubMed  CAS  Google Scholar 

  8. Marcus R, Aurbach GD (1971) Adenyl cyclase from renal cortex. Biochim Biophys Acta 242:410–421

    Article  PubMed  CAS  Google Scholar 

  9. Marcus R, Aurbach GD (1969) Bioassay of parathyroid hormone in vitro with a stable preparation of adenylate cyclase from rat kidney. Endocrinology 85:801–810

    Article  PubMed  CAS  Google Scholar 

  10. Di Bella FP, Dousa TP, Miller SS, Arnaud CD (1974) Parathyroid hormone receptors of renal cortex — specific binding of biologically active, 125I-labeled hormone and relationship to adenylate cyclase activation. Proc Natl Acad Sci USA 71:723–726

    Article  PubMed  Google Scholar 

  11. Mohr H, Hesch RD (1980) Different handling of parathyrin by basal-lateral and brush-border membrane of the bovine kidney cortex. Biochem J 188: 649–656

    PubMed  CAS  Google Scholar 

  12. Zull JE, Chuang J (1985) Characterization of parathyroid hormone fragments produced by Cathepsin D. J Biol Chem 260:1608–1613

    PubMed  CAS  Google Scholar 

  13. Martin TJ, Vakakis N, Eismann JA, Livesey SJ, Tregaer GW (1974). Chick kidney adenylate cyclase: sensitivity of parathyroid hormone and synthetic human and bovine peptides. J Endocrinol 63:369–375

    Article  PubMed  CAS  Google Scholar 

  14. Nissenson RA, Nyiredy KO, Arnaud CD (1981) Guanyl nucleotide potentiation of parathyroid hormone stimulated adenylate cyclase in chicken renal plasma membranes: a receptor independent effect. Endocrinology 108:1949–1953

    Article  PubMed  CAS  Google Scholar 

  15. Seshsadri MS, Chan YL, Wilkinson MR, Mason RS, Posen S (1985) Some problems associated with adenylate cyclase bioassays for parathyroid hormone. Clin Sci 68:311–319

    Google Scholar 

  16. Segre GV, Rosenblatt M, Reiner BL, Mahaffey JE, Potts JT Jr (1981) Characterization of parathyroid hormone receptors in canine renal cortical plasma membranes using a radioiodinate sulfur-free hormone analogue. J Biol Chem 254:6980–6986

    Google Scholar 

  17. Liang CT, Sacktor B (1977) Preparation of renal cortex basal-lateral and brush border membranes—localization of adenylate cyclase and guanylate cyclase activities. Biochim Biophys Acta 466:474–487

    Article  PubMed  CAS  Google Scholar 

  18. Bader CA, Monet JD, Rivaille P, Gaubert CM, Moukhtar MS, Milhaud G, Funck-Brentano JL (1976) Comparative in vitro biological activity of 1–34 N-terminal synthetic fragments of human parathyroid hormone on bovine and porcine kidney membranes. Endocr Res Commun 3:167–186

    Article  PubMed  CAS  Google Scholar 

  19. Jackson BA, Hui YSF, Northrup TE, Dousa TP (1980) Differential responsiveness of adenylate cyclase from rat, dog, and rabbit kidney to parathyroid hormone, vasopressin and calcitonin. Miner Electrolyte Metab 3:136–145

    CAS  Google Scholar 

  20. Sraer J, Sraer JD, Chansel D, Jiüppner H, Hesch RD, Ardaillou R (1978) Evidence for glomerular receptors for parathyroid hormone. Am J Physiol 235:F96-F103

    Google Scholar 

  21. Somkuti SG, Hildebrandt JD, Herberg JT, Iyengar R (1982) Divalent cation regulation of adenylate cyclase. J Biol Chem 257:6387–6393

    PubMed  CAS  Google Scholar 

  22. Bellorin-Font E, Martin KJ (1981) Regulation of the PTH-receptor-cyclase system of canine kidney: effects of calcium, magnesium and guanine nucleotides. Am J Physiol 241:F364–F373

    Google Scholar 

  23. Hunt NH, Martin TJ, Michelangeli VP, Eismann JA (1976) Effect of guanyl nucleotides on parathyroid hormone-responsive adenylate cyclase in chick kidney. J Endocrinol 69:401–412

    Article  PubMed  CAS  Google Scholar 

  24. Nissenson RA, Abbott SR, Teilelbaum AP, Clark OH, Arnaud CD (1981) Endogenous biologically active human parathyroid hormone: measurement by a guanyl nucleotide amplified renal adenylate cyclase assay. J Clin Endocrinol Metab 52:840–846

    Article  PubMed  CAS  Google Scholar 

  25. Marx SJ, Fedak SA, Aurbach GS (1972) Preparation and characterization of a hormone-responsive renal plasma membrane fraction. J Biol Chem 247:6913–6918

    PubMed  CAS  Google Scholar 

  26. Hebert GA, Pelham PL, Pittman B (1972) Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera. App Microbiol 25:26–36

    Google Scholar 

  27. Di Bella FP, Arnaud CD, Brewer HB Jr (1976) Relative biologic activities of human and bovine parathyroid hormones and their synthetic NH2-terminal (1–34) peptides, as evaluated in vitro with renal cortical adenylate cyclase obtained from three different species. Endocrinology 99:429–436

    Article  PubMed  Google Scholar 

  28. Londos C, Salomon Y, Lin MC, Harwood JP, Schramm M, Wolff J, Rodbell M (1974) 5′-Guanylimidodiphosphate, a potent activator of adenylate cyclase systems in eukaryotic cells. Proc Natl Acad Sci USA 71:3087–3090

    Article  PubMed  CAS  Google Scholar 

  29. Auf’mkolk B (1983) Untersuchungen zur Charakterisierung des biologisch aktiven Parathormons im Menschen mit einem optimierten Adenylatcyclase-Bioassay. Dissertation, University Hannover

    Google Scholar 

Download references

Authors
  1. H. Scharfenstein
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Im Breitspiel 15, D-6900, Heidelberg, Germany

    Heinrich Schmidt-Gayk

  2. Immundiagnostik GmbH, Wilhelmstraße 7, D-6140, Bensheim, Germany

    Franz Paul Armbruster Dipl.Ing. (chem.)

  3. Laboratorium voor Experimentele Geneeskunde en Endocrinologie (LEGENDO), Katholieke Universiteit Leuven, Gasthuisberg, B-3000, Leuven, Germany

    Roger Bouillon

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Scharfenstein, H. (1990). Sensitive Adenylate Cyclase Bioassay for Parathyroid Hormone. In: Schmidt-Gayk, H., Armbruster, F.P., Bouillon, R. (eds) Calcium Regulating Hormones, Vitamin D Metabolites, and Cyclic AMP Assays and Their Clinical Application. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-00406-7_14

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-00406-7_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-00408-1

  • Online ISBN: 978-3-662-00406-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation