Fluorescein, resorufin, and rhodamine derivatives are three important fluorescence labels which can be used for labeling of biomolecules. Although there are many more fluorescence labels, which differ in excitation/emission wavelengths, e.g., coumarins, this chapter concentrates mainly on the most popular labels because of their widespread application.
KeywordsFluorescence Label Brane Probe Fluorescence Immunoassay Rhodamine Derivative Prehybridization Buffer
Unable to display preview. Download preview PDF.
- Haugland EP (1982) Covalent fluorescent probes. In: Excited States of Biopolymers. Steiner RF (ed), Plenum Press, New York, LondonGoogle Scholar
- Herrmann R, Josel H-P, Wörner W, Fetterhof TJ (1989) Conjugation of proteins to var- ious fluorescence labels, 19th FEBS meeting, Rom, Abstract Nr FR 511Google Scholar
- Raffael A (1988) GIT Labor-Medizin, 89–97Google Scholar
- Diamandis EP, Christopoulos TK, Bean CC (1992) Quantitative western blot analysis and spot immunodetection using time-resolved fluorometry J Immunol Methods 147: 251–259Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual. 2nd EditionGoogle Scholar