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Abstract

One of the most sensitive and widespread colorimetric detection systems in matrix-based application formats is the redox couple 5-bromo-4-chloro3-indolyl phosphate (BCIP)/nitroblue tetrazolium chloride (NBT). It is used for the detection and localization of alkaline phosphatase activity.

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© 1992 Springer-Verlag Berlin Heidelberg

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Guder, HJ., Kunz, W., West, S. (1992). Colorimetric Systems. In: Kessler, C. (eds) Nonradioactive Labeling and Detection of Biomolecules. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-00144-8_12

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  • DOI: https://doi.org/10.1007/978-3-662-00144-8_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-00146-2

  • Online ISBN: 978-3-662-00144-8

  • eBook Packages: Springer Book Archive

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