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General Aspects of Nonradioactive Labeling and Detection

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Book cover Nonradioactive Labeling and Detection of Biomolecules

Part of the book series: Springer Laboratory ((SLM))

Abstract

In the course of the past decade, increasing attempts to detect basic biological substances such as nucleic acids, proteins, and glycans by nonradioactive bioanalytical indicator systems have been made. In addition to special techniques such as nucleic acid detection via ethidium bromide intercalation (Bauer and Vinograd, 1968; Nathans and Smith, 1975; Ausubel et al., 1987), protein visualization by Coomassie staining (Bennett and Scott, 1971; Zehr et al., 1989), or the detection of glycans by periodate-Schiff staining (Kapitany and Zebrowski, 1973), an increasing number of indicator systems are being developed which are characterized by a higher specificity and sensitivity. Analytical systems which permit the analysis of different biomolecules by uniform detection principles are of particular interest.

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  1. Christoph Kessler
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  1. Forschungszentrum Penzberg, Boehringer Mannheim GmbH, Nonnenwald 2, W-8122, Penzberg, Germany

    Christoph Kessler

  2. Institut für Biochemie, Ludwig-Maximilians-Universität München, Karlstr. 21, W-8000, München 2, Germany

    Christoph Kessler

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© 1992 Springer-Verlag Berlin Heidelberg

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Kessler, C. (1992). General Aspects of Nonradioactive Labeling and Detection. In: Kessler, C. (eds) Nonradioactive Labeling and Detection of Biomolecules. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-00144-8_1

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  • DOI: https://doi.org/10.1007/978-3-662-00144-8_1

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