Abstract
For random mutagenesis of thal adjustment of the mutation rate during epPCR is an essential precondition to obtain high-quality mutant libraries suitable for the directed enzyme evolution. A mutation frequency of 2-7 nucleotide mismatches per gene is recommended for the initial mutation approach. Regarding the 1.6 kb thal gene a mutation rate of 2 mutations/kb was desired and therefore epPCR optimized with regard to this target.
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© 2017 Springer Fachmedien Wiesbaden GmbH
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Minges, H. (2017). Directed evolution of Thal: Results. In: Engineering of Halogenases towards Synthetic Applications. BestMasters. Springer Spektrum, Wiesbaden. https://doi.org/10.1007/978-3-658-18410-0_4
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DOI: https://doi.org/10.1007/978-3-658-18410-0_4
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Publisher Name: Springer Spektrum, Wiesbaden
Print ISBN: 978-3-658-18409-4
Online ISBN: 978-3-658-18410-0
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