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Directed evolution of Thal: Results

  • Hannah Minges
Chapter
Part of the BestMasters book series (BEST)

Abstract

For random mutagenesis of thal adjustment of the mutation rate during epPCR is an essential precondition to obtain high-quality mutant libraries suitable for the directed enzyme evolution. A mutation frequency of 2-7 nucleotide mismatches per gene is recommended for the initial mutation approach. Regarding the 1.6 kb thal gene a mutation rate of 2 mutations/kb was desired and therefore epPCR optimized with regard to this target.

Keywords

Random Mutagenesis Elution Fraction Crude Lysate Relative Conversion Preparative Synthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Fachmedien Wiesbaden GmbH 2017

Authors and Affiliations

  1. 1.BielefeldGermany

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