Abstract
HPLC is a versatile technique for the separation, purification and characterization of different compounds, e. g. macromolecules, such as peptides and proteins, but also amino acids, metabolites etc. can be examined. The principle of separation is based on the analyte’s different adsorption to the stationary phase and its interaction with the mobile phase. In normal phase HPLC, a polar stationary phase generally consisting of silica media and a non-polar mobile phase are used, whereas RP-HPLC employs a non-polar stationary phase and elution is performed with polar solvents.
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Minges, H. (2017). Material and Methods. In: Engineering of Halogenases towards Synthetic Applications. BestMasters. Springer Spektrum, Wiesbaden. https://doi.org/10.1007/978-3-658-18410-0_3
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DOI: https://doi.org/10.1007/978-3-658-18410-0_3
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