Abstract
In mammals, melanin is synthesized in a specialized secretory cell known as the melanocyte. This cell is characterized by the presence of tyrosinase, which is responsible for the aerobic oxidation of L-tyrosine to dopa and to dopaquinone, which in turn is converted to indole-5,6-quinone. This monomer then is oxidized and polymerized to form a large polymer which is believed to be attached, through its quinone linkages, to amino or sulfhydryl groups of the protein matrix of the pigment granules [8, 10]. Recent electron microscopic studies show that the melanin granules appear to start in a region of the melanocyte associated with the Golgi membranes as hollow vesicles in which a tenuous material appears in the form of a folded lamella. This material is rapidly thickened and defined by the deposition of more dense material which is considered to be polymerized indole-5,6quinone, although the nature of this material is not yet thoroughly determined [2,22]. On the other hand, morphological and biochemical studies have revealed that in the secretory cell, the rough membranes and the smooth membranes which are main components of the small granule fraction when it is fractionated by biochemical procedures, were all engaged in the formation of secretory substances and in the secretory mechanisms [23].
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References
Allfrey, V. G., A. E. Mirsky, and S. Osawa: Protein synthesis in isolated cell nuclei. J. gen. Physiol. 40, 451–490 (1957).
Birbeck, M. S. C.: Electronmicroscopy of melanocytes. The fine structure of hair-bulb premelanosomes. Ann. N. Y. Acad. Sci. 100, 540–547 (1963).
Blols, JR., M. S., and R. F. Kallman: The incorporation of C14 from 3,4¬dihydroxyphenylalanine-2’-C14 into the melanin of mouse melanomas. Cancer Res. 24, 863–868 (1964).
Dallner, G., S. Orrenius, and A. Bergstrand: Isolation and properties of rough and smooth vesicles from rat liver. J. Cell Biol. 16, 426–430 (1962).
Ernster, L., P. Siekevitz, and G. Palade: Enzyme-structure relationships in the endoplasmic reticulum of rat liver; a morphological and biochemical study. J. Cell. Biol. 15, 541–562 (1962).
Gianetto, R., and C. de Dove: Tissue fractionation studies; comparative study of the binding of acid phosphatase, ß-glucuronidase and cathepsin by rat-liver particles. Biochem. J. 59, 433–438 (1955).
Greenberg, S. S., and M. J. Kopac: Studies of gene action and melanogenic enzyme activity in melanomatous fishes. Ann. N. Y. Acad. Sci. 100, 887–923 (1963).
Harley Mason, J.: Biosynthesis and structure of tyrosine melanin. In International Congress of Pure and Applied Chemistry XVII, München 1959. London: Butterworth 1960, p. 35–39.
Johnson, M. J.: Isolation and properties of a pure yeast polypeptidase. J. Biol. Chem. 137, 575–586 (1941).
Mason, H. S.: The chemistry of melanin. III. Mechanism of the oxidation of dihydroxyphenylalanine by tyrosinase. J. Biol. Chem. 172, 83–99 (1948).
Miyamoto, M., and T. B. Fitzpatrick: On the nature of the pigment in retinal pigmented epithelium. Science 126, 449 (1957).
Nakai, T., and P. Shusix: Electronmicroscopic radioautography: the melanosome as a site of melanogenesis in neoplastic melanocytes. J. invest. Derm. 43, 267–269 (1964).
Ogata, K., I. Watanabe, T. Morita, and H. Sugano: Decrease in the incorporating activity of liver ribosomes and the release of ribonucleic acid as a result of ultrasonic treatment. Biochim. biophys Acta. 55, 264–267 (1962).
Palade, G. E., and P. Siekevitz: Liver microsomes. An integrated morphological and biochemical study. J. biophys. biochem. Cytol. 2, 171–200 (1956).
Peters, T., Jr.: The biosynthesis of rat serum albumin. I. Properties of rat albumin and its occurrence in liver cell fraction. J. biol. Chem. 237, 1181–1185 (1962).
Rothschild, J. A.: Sub-fraction of rat liver microsomes. Fed. Proc. 20, 145 (1961).
Schneider, W. C., and G. H. Hogeboom: Intracellular distribution of enzymes. VII. The distribution of nucleic acids and adenosinetriphosphatase in normal mouse liver and mouse hepatoma. J. Nat. Cancer Inst. 10, 977–982 (1950).
Seiji, M., T. B. Fitzpatrick, R. T. Simpson, and M. S. C. Birbeck: Chemical composition and terminology of specialized organelles (melanosomes and melanin granules) in mammalian melanocytes. Nature 197, 1082–1084 (1963).
Seiji, M., and S. Iwashita: Intracellular localization of tyrosinase in melanocyte. J. Biochem. 54, 103–106 (1963).
Seiji, M., and S. Iwashita: On the site of melanin formation in melanocytes. J. Biochem. 54, 465–467 (1963).
Seiji, M., and K. Shimao: Density gradient centrifugation. Japanese J. Biochem. 33, 435–441 (1961).
Seiji, M., and K. Shimao, M. S. C. Birbeck, and T. B. Fitzpatrick: Subcellular localization of melanin biosynthesis. Ann. N. Y. Acad. Sci. 100, 497–544 (1963).
Siekevitz, P., and G. E. Palade: A cytochemical study of the pancreas of the guinea pig. II. Functional variations in the enzymatic activity of microsomes. J. biophys. biochem. Cytol. 4, 401–410 (1958).
Swanson, M. A.: Glucose-6-phosphatase from liver. In: Method in Enzymology. Ed. S. P. Colowicx and H. O. Kaplan. New York: Academic Press 1955.
van Lancker, J. L., and R. L. Holtzer: Tissue fractionation studies of mouse pancreas; intracellular distribution of nitrogen, deoxyribonucleic acid, ribonucleic acid, amylase, acid phosphatase, deoxyribonuclease and cytochrome oxidase. J. biol. Chem. 234, 2359–2363 (1959).
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Seiji, M. (1966). Subcellular Tyrosinase Activity and Site of Melanogenesis in Melanocytes. In: Porta, G.D., Mühlbock, O. (eds) Structure and Control of the Melanocyte. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-99906-2_16
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DOI: https://doi.org/10.1007/978-3-642-99906-2_16
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