Abstract
Cryotechniques do not require tissue to be stabilised by fixatives prior to resin embedding, and so are useful for preserving antigenic sites that are sensitive to even low concentrations of fixative, or for preserving structures that cannot be stabilised during fixation or dehydration (Sect. 1.3.2). The prerequisite for cryotechniques is for biological tissue to be rapidly frozen prior to any processing steps. Processing can then be in to resin (cryosubstitution, Sect. 4.3; freeze-drying, Sect. 4.6), or the frozen tissue can be sectioned directly (cryoultramicrotomy, Sect. 4.9).
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© 1993 Springer-Verlag Berlin Heidelberg
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Newman, G.R., Hobot, J.A. (1993). Cryotechniques. In: Resin Microscopy and On-Section Immunocytochemistry. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-97481-6_4
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DOI: https://doi.org/10.1007/978-3-642-97481-6_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-97483-0
Online ISBN: 978-3-642-97481-6
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