Skip to main content

Isolated Pancreas Islet Xenografting

  • Chapter

Abstract

At present, short-term success has been obtained with isolated pancreas islet allografts [1,2]. Clinical success has not been obtained with islet xenografts, although at least one study suggest that the human immune response to islet xenografts may be no stronger than that to allografts in some instances [3]. Recent advances in both islet isolation techniques and suppression of xenogeneic immune reactivity have provided a measure of optimism regarding the future of islet xenografting which was not previously appropriate.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   74.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Scharp, D.W., Lacy, P.E., Santiago, J.V., McCullough, C.S., Weide, L.G., Falqui, L., Marchetti, P., Gingerich, R.L., Jaffe, A.S., Cryer, P.E., Anderson, C.B., Flye, M.W. Insulin independence after islet transplantation into type I diabetic patient. Diabetes. 39, 515, 1990.

    Article  PubMed  CAS  Google Scholar 

  2. Tzakis, A.G, Ricordi, C., Alejandro, R., Zeng, Y., Fung, J.J., Todo, S., Demetris, A.J., Mintz, D.H., Starzl, T.E. Pancreatic islet transplantation after upper abdominal exenteration and liver replacement. Lancet. 336,402, 1990.

    Article  PubMed  CAS  Google Scholar 

  3. Kondratiev, Y.Y., Sodovnikova, N.V., Petrova, G.N., Fedotov, V.P., Bljumkin, Y.N., Ignatenko, S.N., Pankov, Y.A. Islet cell transplantation in type I diabetes mellitus: evaluation of humoral immune response. Exp. Clin. Endocrinol. 93,147, 1989.

    Article  PubMed  CAS  Google Scholar 

  4. Weber, C., Zatrigi, A., Weil, R., Mcintosh, R., Hardy, M.A., Reemtsma, K. Pancreatic islet isografts, allografts, and xenografts: comparison of morphology and function. Surgery. 79,144, 1976.

    PubMed  CAS  Google Scholar 

  5. Williams, P.W. Notes on diabetes treated with grafts of sheep’s pancreas. Br. Med. J. 2, 1303, 1893.

    Article  Google Scholar 

  6. Weber, C., Weil, R., Mcintosh, R., Hogle, H., Warden, G., Reemtsma, K. Xenotransplantation of piscine islets into hyperglycemic rats. Surgery. 77,208, 1975.

    PubMed  CAS  Google Scholar 

  7. Eloy, R., Haffen, K., Kedinger, M., Grenier, J.F. Chick embryo pancreatic transplants reverse experimental diabetes of rats. J. Clin. Invest. 64,361, 1979.

    Article  PubMed  CAS  Google Scholar 

  8. Lacy, P.E., Davie, J.M., Finke, E.H. Prolongation of islet xenograft survival without continuous immunosuppression. Science. 209,283, 1980.

    Article  PubMed  CAS  Google Scholar 

  9. Reckard, C.R., Stuart, F., Schulak, J.A. Immunologic comparisons of isolated pancreatic islet and whole-organ allografts. Transplant. Proc. 11,563, 1979.

    PubMed  CAS  Google Scholar 

  10. Kaufman, D.B., Rabe, F.L., Platt, J.L., Dunn, D.L., Sutherland, D.E.R. Effect of host immunomodulation on the prevention of islet allograft primary non-function in a murine model. Transplant. Proc. 22,857, 1990.

    PubMed  CAS  Google Scholar 

  11. Lacy, P.E., Kostianovsky, M. A method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes. 16,35, 1967.

    PubMed  CAS  Google Scholar 

  12. Sutherland, D.E.R., Matas, A.J., Najarian, J.S. Pancreas islet cell transplantation. Surg. C/m.N. Amer. 58,365, 1978.

    CAS  Google Scholar 

  13. Morris, P.J., Gray, D.W., Sutton, R. Pancreatic islet transplantation. Br. Med. Bull. 49, 224, 1989.

    Google Scholar 

  14. Ricordi, C., Lacy, P.E., Finke, E.H., Olack, B.J., Scharp, D.W. Automated method for isolation of human pancreatic islets. Diabetes. 37,413, 1988.

    Article  PubMed  CAS  Google Scholar 

  15. Ricordi, C., Socci, C., Davalh, A.M., Staudacher, C., Baro, P., Vertova, A., Sassi, I., Gavazzi, F., Pozza, G., DiCarlo, V. Isolation of the elusive pig islet. Surgery. 107,688, 1990.

    PubMed  CAS  Google Scholar 

  16. Calafiore, R., Basta, G., Calcinaro, F., Falorni, A., Pietropaolo, M., Avellini, L., Brunetti, P. Islands of Langerhans transplant in the treatment of diabetes mellitus: current status, indications, future perspectives. Recenti. Prog. Med. 81,94, 1990.

    PubMed  CAS  Google Scholar 

  17. Hering, B.J., Romann, D., Clarius, A., Brendel, M., Bretzel, R.G., Federlin, K. Bovine islets of Langerhans: potential source for transplantation? Diabetes. 38,206, 1982.

    Google Scholar 

  18. Hering, B.J., Romann, D., Clarius, A., Brendel, M., Bretzel, R.G., Federlin, K. Islands of Langerhans of cattle - potential donor tissue for chnical islet transplantation? Z. Exp. Chir. Transplant. Kiinstliche Organe. 22,138, 1989.

    CAS  Google Scholar 

  19. Bonner-Weir, S., Fike, A.A. A dual population of islets of Langerhans in bovine pancreas. Cell Tissue Res. 206,157, 1980.

    Article  PubMed  CAS  Google Scholar 

  20. Alejandro, R., Strasser, S., Zucker, P.F., Mintz, D.H. Isolation of pancreatic islets from dogs. Semi-automated purification on albumin gradients. Transplantation. 50, 207, 1990.

    Article  PubMed  CAS  Google Scholar 

  21. Finegood, D.L., Warnock, G.L., Kneteman, N.M., Rajotte, R.V. Insuhn sensitivity and glucose effectiveness in long-term islet autotransplanted dogs. Diabetes. 38,189, 1989.

    PubMed  CAS  Google Scholar 

  22. Rajotte, R.V., Warnock, G.L., Kneteman, Erickson, C., Ellis, D.K. Optimizing cryop– reservation of isolated islets. Transplant. Proc. 21,2638, 1989.

    PubMed  CAS  Google Scholar 

  23. Tzu, W.J., Tai, J. Xenotransplantation of rat pancreatic endocrine cells in spontaneous and streptozotocin-induced diabetic monkeys. Transplant. Proc. 21,2736, 1989.

    Google Scholar 

  24. Selawry, H.P., Whittington, K.B., Bellgrau, D. Abdominal intratesticular islet-xenograft survival in rats. Diabetes. 38,220, 1989.

    PubMed  Google Scholar 

  25. Naji, A. Personal communication, December, 1990

    Google Scholar 

  26. Ricker, A.T., Stockberger, S.M., Halban, P. A. In: The Immunology of Diabetes Mellitus. Jaworski, M.A. (ed.) Amsterdam, Elsevier, 1986, p. 193.

    Google Scholar 

  27. O’Shea, G.M., Sun, A.M. Encapsulation of rat islets of Langerhans prolongs xenograft survival in diabetic mice. Diabetes. 35,943, 1986.

    Article  PubMed  Google Scholar 

  28. Siebers, U., Zekorn, T., Bretzel, R.G., Renardy, M., Zschocke, P., Planck, H., Federlin, K. Bioartificial pancreas: islet survival and interleukin-1 action. Transplant. Proc. 22, 2035, 1990.

    PubMed  CAS  Google Scholar 

  29. Wu, Z.G., Shi, Z.Q., Lu, Z.N., Yang, H., Shi, F.Y., Zheng, X.R., Sun, A.M. In vitro culture and transplantation of encapsulated human fetal islets as an artificial endocrine pancreas. Trans. Am. Soc. Artif Intern. Organs. 35,736, 1989.

    Article  CAS  Google Scholar 

  30. Weber, C., Zabinski, S., Koschitzky, T., Wicher, L., Rajotte, R., D’Agati, V., Peterson, L., Norton, J., Reemtsma, K. The role of CD4+ helper T cells in the destruction of microencapsulated islet xenografts in NOD mice. Transplantation. 49,396, 1990.

    Article  PubMed  CAS  Google Scholar 

  31. Lafferty, K.J., Cooley, M., Woolnough, J., Walker, K.Z. Thyroid allograft immunogenicity is reduced after organ culture. Science. 188,259, 1975.

    Article  PubMed  CAS  Google Scholar 

  32. Sollinger, H.W., Burkenholder, P.M., Rasmus, W.R., Bach, F.H. Prolonged survival of xenografts after organ culture. Surgery. 81,74, 1977.

    PubMed  CAS  Google Scholar 

  33. Lacy, P.E., Davie, J.M., Finke, H., Scharp, D.W. Prolongation of islet allograft survival. Transplantation. 27,171, 1979.

    Article  PubMed  CAS  Google Scholar 

  34. Stock, P.G., Ascher, N.L., Chen, S., Bumgardner, G., Field, M.J., Sutherland, D.E. The alloimmune response to murine islets occurs via indirect antigen presentation to LYT2+ and L3T4+ lymphocytes. Transplant. Proc. 22,841, 1990.

    PubMed  CAS  Google Scholar 

  35. Auchincloss, H. Xenotransplantation-a review. Transplant. Rev. 46,1, 1990.

    Google Scholar 

  36. Thomas, F., Pittman, K. Factors in effective isolation and culture of adult pig islets (API). Submitted for publication.

    Google Scholar 

  37. Coulombe, M., Warnock, G., Rajotte, R. Prolongation of islet xenograft survival by cry– opreservation. Diabetes. 36,1086, 1987.

    Article  PubMed  CAS  Google Scholar 

  38. Lafferty, K., Davie, J.M., Finke, E. Prolongation of islet xenograft survival (rat-to– mouse). Diabetes. 30,285, 1981.

    Google Scholar 

  39. Faustman, D.L., Stienman, R.M., Gabel, A.M. Depletion of anti-la + dendritic cells. Proc. Natl. Acad. Sci. USA. 81,3864, 1984.

    Article  PubMed  CAS  Google Scholar 

  40. Larsen, C., Austyn, J., Morris, P. The role of graft-derived dendritic leukocytes in the rejection of vascularized organ allografts. An«. Surg. 212,308, 1990.

    CAS  Google Scholar 

  41. Chabot, J., Weber, C., Hardy, M., Rivera, S., Bailey-Braxton, D., Strausberg, L., Wood, M, Chow, J., Pl-Sunyer, F.X., Reemtsma, K. Synergy of ALS and UV-B in prolongation ofprimate-to-mouse islet xenograft survival. Transplant. Proc. 19,1160, 1987.

    PubMed  CAS  Google Scholar 

  42. Walter, P., Lesmeister, F., Leetz, H., Schnabel, K., Feifel, G., Jaeger, S. Influence of and UVB irradiation on graft survival and function of islets of Langerhans. Diabetes. 38, Suppl. 1,288, 1989.

    Google Scholar 

  43. Ricordi, C., Lacy, P.E., Sterbenz, K., Davie, J.M. Low temperature culture of human islets or in vivo treatment with L3T4 antibody produces a marked prolongation of human-to-mouse islet xenograft survival. Proc. Natl. Acad. Sci. USA. 84,8080, 1987.

    Article  PubMed  CAS  Google Scholar 

  44. Has, L., Lafferty, K., Allison, A., Eugui, E.M. RS-61443 allows islet allografting and specific tolerance induction in adult mice. Transplant. Proc. 22,876, 1990.

    Google Scholar 

  45. Lacy, P.E. Islet transplantation. Clin. Chem. 32/lOB, B76, 1986.

    Google Scholar 

  46. Kruszinska, Y., Hone, D., Morley, A., Alberti, A. Preservation of islet function and morphology in high dose streptozotocin diabetic rats. Diab. Res. 3,175, 1986.

    Google Scholar 

  47. Trimble, E., Karakash, C., Malaisse-Lagae, R. Effects of intraportal islet transplantation on the transplanted tissue and recipient pancreas. Diabetes. 29,341, 1980.

    Article  PubMed  CAS  Google Scholar 

  48. Richardt, M., Menden, A, Bretzel, R., Federlin, K. Islet transplantation for experimental diabetes of the rat. B cell regeneration following transplant. Horm. Metab. Res. 16,551, 1984.

    Article  PubMed  CAS  Google Scholar 

  49. Slipjepcevic, M., Helmke, K., Federlin, K. Islet transplantation in experimental diabetes of the rat. Horm. Metab. Res. 7,20, 1975.

    Article  Google Scholar 

  50. Chicheportiche, D., Darquy, S., Lepeintre, J., Capron, F., Halban, P.A., Reach, G. High– performance liquid chromotography analysis of circulating insulins distinguishes between endogenous insulin production (a potential pitfall with streptozotocin diabetic rats) and islet xenograft function. Diabetologia. 33,457, 1990.

    Article  PubMed  CAS  Google Scholar 

  51. Terasaka, R., Lacy, P.E., Bucy, R.P., Davie, J.M. Effect of cyclosporine and low temperature culture on rejection of islet xenografts (rat-to-mouse). Transplantation. 41,661, 1986.

    Article  PubMed  CAS  Google Scholar 

  52. Stegall, M., Chabot, J, Weber, C., Reemtsma, K., Hardy, M.A. Pancreatic islet transplantation in cynomolgus monkeys. Transplantation. 48,944, 1989.

    Article  PubMed  CAS  Google Scholar 

  53. Nakajima, Y., Nakano, H., Nakagawa, H., Segawa, M., Shiratori, T. Effect of total lymphoid irradiation on pancreas islet xenograft survival in rats. Transplantation. 34, 98, 1982.

    Article  PubMed  CAS  Google Scholar 

  54. Lacy, P.E. Present status of islet transplantation. Clin. Inv. Med. 10,496, 1987.

    CAS  Google Scholar 

  55. Simeonovic, C., Ceredio, R., Wilson, J.D. Effect of GK1.5 antibody dosage on survival of pig proislet xenografts in CD4+ T cell depleted mice. Transplantation. 49,849, 1990.

    Article  PubMed  CAS  Google Scholar 

  56. Yosunami, Y., RYU, S., Kamei, T. Characterization of immunosuppressive activity of FK506 in rat islet allografts. Transplant Proc. 22,875, 1990.

    Google Scholar 

  57. Pittman, K., Thomas, F.T., Patselas, T., Thomas, J.M. Long-term (12–14 week) survival of pig islet xenografts (PIX) in Lewis rats (LR) treated with splenectomy (SPL), deoxyspergualin (DSG) and rabbit antithymocyte globulin (RATG). Submitted for publication.

    Google Scholar 

  58. Thomas, F. Comparative immunosuppression for xenografting. In: Xenograft25. Hardy, M.A. (ed.) Elsevier; Amsterdam, New York, Oxford, 1989, p. 55.

    Google Scholar 

  59. Naji, A., Barker, C. Differential susceptibility of endocrine tissues to humoral immunity. Surg. Forum. 29,349, 1978.

    PubMed  CAS  Google Scholar 

  60. Delmonico, F., Chase, C., Russell, P. Transplantation of rat islets of Langerhans into diabetic mice. Transplant. Proc. 9,367, 1977.

    PubMed  CAS  Google Scholar 

  61. Sutton, R., Greay, D.W., Burnett, M., McShane, P., Turner, R., Morris, P. Metabolic function of intraportal and intrasplenic islet autografts in cynomolgus monkeys. Diabetes. 38,182, 1989.

    Article  PubMed  Google Scholar 

  62. Alejandro, R., Cutfield, R.G., Steinvold, F., Polonsky, K.S., Noel, J., Olson, L., Dillberger, J., Miller, J., Mintz, D.H. Natural history of intrahepatic canine islet cell autografts. Clin. Invest. 78,1339, 1986.

    Article  CAS  Google Scholar 

  63. Warnock, G.L., Rajotte, V.R., Procyshyn, A.W. Normoglycemia after reflux of islet-containing pancreas fragments into the splenic vascular bed in dogs. Diabetes. 32,452, 1983.

    Article  PubMed  CAS  Google Scholar 

  64. Kaufman, D., Morel, D., Field, M., Tzardis, P., Munn, S.R., Sutherland, D.E. Canine islet autografts. Functional outcome as influenced by islet number and purity. Transplant. Proc. 22,771, 1990.

    PubMed  CAS  Google Scholar 

  65. Kanai, T., Porter, J., Monaco, A.P., Maki, T. Sequential multiple transplantations of multiple-donor pancreatic islet allografts. Diabetes. 38, Suppl. 1,286, 1989.

    Google Scholar 

  66. Alejandro, R., Mintz, D.H., Noel, J., Latif, Z., Koh, N., Russell, E., Miller, J. Islet cell transplantation in type I diabetes mellitus. Transplant. Froc. 19,2359, 1987.

    CAS  Google Scholar 

  67. Scharp, D.W., Lacy, P., Ricordi, C., Boyle, P., Santiago, J., Cryer, P., Gingerick, R., Jaffe, A., Anderson, C., Flye, W. Human islet transplantation in patients with type I diabetes. Transplant. Proc. 21,2744, 1989.

    PubMed  CAS  Google Scholar 

  68. Cicciarelli, J., Cho, R.V. Regraft kidney transplant survival. In: Clinical Transplants. Terasaki, P.I. (ed.) Los Angeles, Ucla Tissue Typing Lab, 1986, p. 285.

    Google Scholar 

  69. Gotoh, M., Porter, J., Kanai, T., Monaco, A.P., Maki, T. Effect of specific and nonspecific alloantigen stimulation on islet allograft survival. Transplant. Proc. 21,2746, 1989.

    PubMed  CAS  Google Scholar 

  70. Kanai, T., Porter, J., Maki, T., Monaco, A.P. Possible mechanisms underlying marked graft survival following multiple donor islet allotransplantation. Transplant. Proc. In press.

    Google Scholar 

  71. Sachs, D., Bach, F. Immunology of xenograft rejection. Hum. Immunol. 28,245, 1990.

    Article  PubMed  CAS  Google Scholar 

  72. Sun, A.M., O’Shea, G.M., Yuan, Z.Y., Ma., F., Cai, Z. Alio– and xenotransplantation of microencapsulated islets of Langerhans (a bioartificial pancreas). In: Advanced Models for Therapy of Insidin Dependent Diabetes. Brunetti, P., Waldhausl, W.K. (eds.) New York, Raven Press, Serono Symposia, 37,303, 1987.

    Google Scholar 

  73. Manns, J.G., Boda, J.M. Insulin release in lambs and aduh sheep. Am. J. Physiol. 212, 747, 1967.

    PubMed  CAS  Google Scholar 

  74. Lacy, P., Ricordi, C., Finke, E. Effect of transplant site and alpha L3T4 treatment on survival of rat, hamster and rabbit islet xenografts in mice. Transplantation. 47,761, 1989.

    Article  PubMed  CAS  Google Scholar 

  75. King, N.W., Chalifoux, L.V., Ringler, D.J., Wyand, M.S., Sehgal, P.K., Daniel, M.D., Letvin, N.L. Comparative biology of natural and experimental SIV mac infection in macaque monkeys: a review. J. Med. Primatol. 19,109, 1990.

    PubMed  CAS  Google Scholar 

  76. Daniel, M.D., Letvin, N.L., Sehgal, P.K., Schmidt, D.K., Silva, D.P., Solomon, K.R., Hodi, F.S. Jr., Ringler, D.J., Hunt, R.D., King, N.W., et al. Prevalence of antibodies to 3 retroviruses in a captive colony of macaque monkeys. Int. J. Cancer. 41,601, 1988.

    Article  PubMed  CAS  Google Scholar 

  77. Sibley, R.K., Sutherland, D.E.R., Goetz, F., Michael, A.F. Recurrent diabetes in the pancreas iso– and allograft. Lab. Invest. 53,132, 1985.

    PubMed  CAS  Google Scholar 

  78. Mandel, T., Koulmanda, M., Loudovaris, T., Bacelj, A. Islet grafts in NOD mice — comparison of iso-alio-and pig xenografts. Transplant. Proc. 21,3813, 1989.

    PubMed  CAS  Google Scholar 

  79. Kaufman, D., Platt, J., Rabe, F., Dunn, D., Bach, F., Sutherland, D.E.R. Differential roles of Moc 1+ cehs and CD4+ and CD8+ T lymphocytes in primary non-function and classic rejection of islet allografts./. Exp. Med. 172,291, 1990.

    Article  CAS  Google Scholar 

  80. Moses, R., Pierson, R. III, Winn, H., Auchincloss, H. Xenogeneic proliferation and lymphokine production are dependent upon CD4+ helper T cells and self-antigen presenting cells in the mouse. J. Exp. Med. 172,567, 1990.

    Article  PubMed  CAS  Google Scholar 

  81. Ildstad, S., Wren, S., Stephany, D., Sachs, D.H. Effect of selective T cell depletions in mixed xenogeneic reconstitution on species hyporeactivity to xenogeneic transplantation. Transplantation. 41,372, 1986.

    Article  PubMed  CAS  Google Scholar 

  82. Crowther, N., Gotfreadsen, C., Moody, A.J., Green, LC. Porcine islet isolation cellular digestion and secretory response. Horm. Met. Res. 21,590, 1989.

    Article  CAS  Google Scholar 

  83. Ricordi, C., Finke, E., Lacy, P.E. A method for the mass isolation of islets from the adult pig pancreas. Diabetes. 35,649, 1986.

    Article  PubMed  CAS  Google Scholar 

  84. Ricordi, C., Socci, A., Davilh, C., Staudacher, C, Vertova, A., Baro, P., Sassi, L, Braghi, S., Giuzzi, N., Pozza, G., DiCarlo, V. Apphcation of the automated method to islet isolation in swine. Transplant. Proc. 22,784, 1990.

    PubMed  CAS  Google Scholar 

  85. Hellerstrom, C., Andersson, A., Korsgren, O., Jansson, L., Sandler, S. Aspects of pancreatic islet transplantation in diabetes. Balliere’s Clin. Gastroenterol. 3,851, 1989.

    Article  CAS  Google Scholar 

  86. Schrezenmeir, J., Faust, P., Laue, C., Kirchgessner, J., Stallmach, T., Müntefering, H., Beyer, J. Immunoprotection in biocompatible membranes permits transplantation of piscine islets to rats. Transplant. Proc. 21,2730, 1989.

    PubMed  CAS  Google Scholar 

  87. Falkmer, S. Ostberg, Y. Comparative morphology of pancreatic islets in animals. In: The Diabetic Pancreas. Volk, B.W., Arquilla, E.R. (eds.) New York, Raven Press, 1977, p. 15.

    Google Scholar 

  88. Alejandro, R., Cutfield, R., Schienvold, F., Polonsky, K.S., Noel, J., Olson, L., Dillberger, J., Miller, J., Mintz, D.H. Natural history of intrahepatic canine islet cell autografts./Clin. Invest. 78,1339, 1986.

    Google Scholar 

  89. Warnock, G., Rajotte, R. Critical mass of purified islets that induce normoglycemia after implantation in the dog. Diabetes. 37,467, 1988.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Thomas, F.T. (1991). Isolated Pancreas Islet Xenografting. In: Cooper, D.K.C., Kemp, E., Reemtsma, K., White, D.J.G. (eds) Xenotransplantation. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-97323-9_16

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-97323-9_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-97325-3

  • Online ISBN: 978-3-642-97323-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics