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Bestimmung der Phospholipase C-Aktivität

nach Kuroshima und Hayano (1982)

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Bodenbiologische Arbeitsmethoden

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Zusammenfassung

Bodenproben werden mit p-Nitrophenylphosphorylcholin (NPPC) als Substrat 2 Stunden bei 30°C inkubiert. Die gebildete Menge an p-Nitrophenol (PNP) wird photometrisch bestimmt.

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Literatur

  • Doi O, Nojima S (1972) Phospholipase C from Pseudomonas fluorescens. Biochim Biophys Acta 248:234–243

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  • Kuroshima T und Hayano K (1982) Phospholipase C Activity in soil. Soil Sci Plant Nutr 28:535–542

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  • Okawa Y, Yamaguchi T (1975) Studies on phospholipase from streptomyces hachijoensis phospholipase C. J Biochem 78:537–545

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Authors and Affiliations

  1. Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25, A-6020, Innsbruck, Austria

    Prof. Dr. Franz Schinner

  2. Bundesanstalt für Agrarbiologie, Wieningerstraße 8, A-4025, Linz, Austria

    Dr. Richard Öhlinger

  3. Bundesanstalt für Bodenwirtschaft, Denisgasse 31-33, A-1200, Wien, Austria

    Dr. Ellen Kandeler

Authors
  1. Prof. Dr. Franz Schinner
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  2. Dr. Richard Öhlinger
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  3. Dr. Ellen Kandeler
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© 1991 Springer-Verlag Berlin Heidelberg

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Schinner, F., Öhlinger, R., Kandeler, E. (1991). Bestimmung der Phospholipase C-Aktivität. In: Bodenbiologische Arbeitsmethoden. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-97284-3_39

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  • DOI: https://doi.org/10.1007/978-3-642-97284-3_39

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-53143-2

  • Online ISBN: 978-3-642-97284-3

  • eBook Packages: Springer Book Archive

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