Techniques of Specimen Collection and Preparation

  • Herbert Begemann
  • Johann Rastetter


Differentiation of the peripheral blood is still the most important procedure in the diagnosis of hematologic disorders. The necessary blood smears are usually prepared from venous blood anticoagulated with EDTA (several brands of collecting tube containing EDTA are available commercially). However, many special tests require that the blood be taken from the fingertip or earlobe and smeared directly onto a glass sHde, with no chemicals added. The slide must be absolutely clean to avoid the introduction of artifacts. Slides are cleaned most effectively by degreasing in alcohol for 24 h, drying with a lint-free cloth, and final wiping with a chamois cloth (as a shortcut, the slide may be scrubbed with 96% alcohol and wiped dry).


Iliac Crest Puncture Site Marrow Cavity Bone Marrow Smear Splenic Cyst 
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  1. 1.
    The interested reader is referred to the Color Atlas of the Clinical Histopathology of Bone Marrow and Bone by R. Burkhardt; Springer, Berlin Heidelberg New York 1970Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Herbert Begemann
    • 1
  • Johann Rastetter
    • 2
  1. 1.I. Medizinischen AbteilungStädtischen Krankenhauses München-SchwabingMünchenFederal Republic of Germany
  2. 2.Abteilung für Hämatologie und Onkologie, I. Medizinische Klinik und Poliklinik, Klinikum rechts der IsarTechnischen Universität MünchenFederal Republic of Germany

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