The Kinetics of Antibiotic Release from a Fibrin-Clotting System: An Animal Experiment

  • R. Pointner
  • J. Kofler
  • Ch. Offer
  • G. Schwab


In colon surgery fibrin glue is commonly used for the additional sealing of high-risk colonic anastomoses. As every anastomosis must be considered contaminated, and fibrin is a culture medium for pathogens, it is natural to suggest antibiotics should be added to the adhesive for its application to an anastomosis.

After preliminary studies concerning the influence of antibiotic combinations on the clotting and adhesive properties of fibrin glue, we investigated the release time of a clindamycin-cefotaxim combination from a fibrin clot under standardized conditions.

One millilitre of fibrin glue was mixed with 25 mg clindamycin and 25 mg cefotaxim, and the fibrin-antibiotic complex was implanted into the free peritoneal cavity of Wistar rats. The clot was removed after 1, 3, 5, 8 and 24h and the remaining quantity of antibiotic in the fibrin clot was measured. Escherichia coli was used for the assessment of cefotaxim release; clindamycin release was tested by Staphylococcus epidermidis. After 24 h we still found sufficiently high concentrations of antibiotic in the fibrin clot. This demonstrates that without impairment of clotting behavior and adhesive qualities of the fibrin glue it is possible to mix the adhesive with an antibiotic combination, which provides for extremely high local concentrations of antibiotic agents by slow release from the fibrin clot.

Key words

Fibrin glue antibiotics diffusion test 


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  1. 1.
    Ahrenholz DH, Simmons RL (1980) Fibrin in peritonitis. Surg 88, 1, 41–47Google Scholar
  2. 2.
    Dunn DL, Rotstein OD, Simmons RL (1984) Fibrin in peritonitis. Arch Surg 119, 139–144PubMedCrossRefGoogle Scholar
  3. 3.
    Kessler B, Arndt M, Zimmermann E, Witting CH (1982) Absicherung von Dickdarmanastomosen mit biogenem Klebematerial. Helv Chir Acta 49, 195–199Google Scholar
  4. 4.
    Langer S, Kupczyk D (1982) Entstehung der Nahtinsuffizienz. Langenbecks Arch Clin 358, 253–258CrossRefGoogle Scholar
  5. 5.
    Marczell A, Efferdinger F, Spoula H, Stierer M (1979) Anwendungsbereiche des Fibrinklebers in der Abdominalchirurgie. Acta Chir Austr 11, 137–141CrossRefGoogle Scholar
  6. 6.
    Oka H, Harrison CR, Burhenne HJ (1982) Effect of a Biologic glue on the leakage rate of experimental rectal anastomoses. Am J Surg 143, 561–564PubMedCrossRefGoogle Scholar
  7. 7.
    Scheele J (1981) Erste klinische Erfahrungen mit der Fibrinklebung bei traumatischer und intraoperativer Milzverletzung. Chirurg 52, 531–534PubMedGoogle Scholar
  8. 8.
    Scheele J, Herzog J, Mühe E (1978) Anastomosensicherung am Verdauungstrakt mit Fibrinkleber. Nahttechnische Grundlagen, experimentelle Befunde, klinische Erfahrungen. Zbl Chirurgie 103, 1325–1336Google Scholar
  9. 9.
    Seidl W, Polterauer P, Funovics J (1982) Blutstillung an parenchymatösen Organen mit Fibrin-Kollagen-Klebung in der Abdominalchirurgie. Acta Chir Austr 14/1, 5–7CrossRefGoogle Scholar
  10. 10.
    Stanek G, Bösch P, Weber P (1978) Vergleichende quantitative Untersuchung des Wachstums von Staphylococcus aureus im Fibrinklebesystem und im Blutkoagulum. Zbl Bact Hyg I Abt Orig A 240, 441–446Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • R. Pointner
  • J. Kofler
  • Ch. Offer
  • G. Schwab

There are no affiliations available

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