Experimental Application of Triple-Labeled Quantitative Autoradiography for Measurement of Cerebral Blood Flow, Glucose Metabolism, and Protein Biosynthesis

  • G. Mies
  • W. Bodsch
  • W. Paschen
  • K.-A. Hossmann


During the past years autoradiographic techniques for measuring blood flow (Reivich et al. 1969; Sakurada et al. 1978) and metabolic activity (Sokoloff et al. 1977) have been of increasing interest in the study of various physiological and pathophysiological states. As long as the experimental procedure can be standardized, correlation of the different parameters is possible using single-label autoradiography, applied to different animals. However, considerable difficulties may be encountered when the parameters of interest change in an unpredictable way. In this case, simultaneous assessment of flow and metabolism in the same brain is a prerequisite for a meaningful evaluation.


Cerebral Blood Flow Brain Section Glucose Consumption Protein Biosynthesis Anaerobic Glycolysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Blasberg RG, Gazendam J, Patlak CS et al. (1980) Quantitative autoradiographic studies of brain edema and a comparison of multi-isotope auto-radiographic techniques. In: Cervós-Navarro J, Ferszt R (eds) Advances in neurology, vol 28: Brain edema. Raven, New York, pp 255–270Google Scholar
  2. 2.
    Bodsch W, Hossmann K-A (to be published) A quantitative regional analysis of amino acids involved in rat brain protein synthesis by high performance liquid chromatography. J Neurochem 40: 371–382Google Scholar
  3. 3.
    Bodsch W, Hurter T, Hossmann K-A (1982) Immunochemical method for quantitative evaluation of vasogenic brain edema following cold injury of rat brain. Brain Res 249: 111–121PubMedCrossRefGoogle Scholar
  4. 4.
    Bodsch W, Mies G, Hossmann K-A (to be published) Simultaneous determination of local cerebral rates of protein synthesis, glucose utilization and blood flow by multi-autoradiographic techniques. Proc Natl Acad SciGoogle Scholar
  5. 5.
    Klatzo I, Chui E, Fujiwara K et al. (1980) Resolution of vasogenic brain edema. Adv Neurol 28: 359–373PubMedGoogle Scholar
  6. 6.
    Kogure K, Alonso OF (1978) A pictorial representation of endogenous brain ATP by a bioluminescent method. Brain Res 154: 273–284PubMedCrossRefGoogle Scholar
  7. 7.
    Lear JL, Jones SC, Greenberg JH, Fedora TJ, Reivich M (1981) Use of 123I and 74C in a double radionuclide autoradiographic technique for simultaneous measurement of LCBF and LCMRgI. Stroke 12: 589–597PubMedCrossRefGoogle Scholar
  8. 8.
    Mies G, Niebuhr I, Hossmann K-A (1981a) Simultaneous measurement of blood flow and glucose metabolism by autoradiographic techniques. Stroke 12: 581–588PubMedCrossRefGoogle Scholar
  9. 9.
    Mies G, Niebuhr I, Hossmann K-A (1981b) A double tracer autoradiographic technique for simultaneous measurement of cerebral blood flow and cerebral metabolism in rats. Eur Neurol 28: 188–193CrossRefGoogle Scholar
  10. 10.
    Paschen W, Djuricic B, Bosma H-J et al. (1981a) Regional blood flow and regional distribution of biochemical substrates in experimental stroke of gerbils. J Cereb Blood Flow Metabol 1 [Suppl 1]: S 174-S 175Google Scholar
  11. 11.
    Paschen W, Niebuhr I, Hossmann K-A (1981b) A bioluminescence method for the demonstration of regional glucose distribution in brain slices. J Neurochem 36: 513–517PubMedCrossRefGoogle Scholar
  12. 12.
    Reivich M, Jehle J, Sokoloff L et al. (1969) Measurement of regional cerebral blood flow with antipyrine-14C in awake cats. J Appl Physiol 27: 296–300PubMedGoogle Scholar
  13. 13.
    Sakurada O, Kennedy C, Jehle J et al. (1978) Measurement of local cerebral blood flow with iodo (14C) antipyrine. Am J Physiol 234: H 59–66Google Scholar
  14. 14.
    Sokoloff L, Reivich M, Kennedy C et al. (1977) The (14C) deoxyglucose method for the measurement of local cerebral glucose utilization: Theory, procedure, and normal values in the conscious and anesthetized albino rat. J Neurochem 28: 897–916PubMedCrossRefGoogle Scholar
  15. 15.
    Welsh FA, Rieder W (1978) Evaluation of in situ freezing of cat brain by NADH fluorescence. J Neurochem 31: 299–309PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • G. Mies
  • W. Bodsch
  • W. Paschen
  • K.-A. Hossmann

There are no affiliations available

Personalised recommendations