Experimental Application of Triple-Labeled Quantitative Autoradiography for Measurement of Cerebral Blood Flow, Glucose Metabolism, and Protein Biosynthesis
During the past years autoradiographic techniques for measuring blood flow (Reivich et al. 1969; Sakurada et al. 1978) and metabolic activity (Sokoloff et al. 1977) have been of increasing interest in the study of various physiological and pathophysiological states. As long as the experimental procedure can be standardized, correlation of the different parameters is possible using single-label autoradiography, applied to different animals. However, considerable difficulties may be encountered when the parameters of interest change in an unpredictable way. In this case, simultaneous assessment of flow and metabolism in the same brain is a prerequisite for a meaningful evaluation.
KeywordsRadionuclide Neurol NADH Halothane Tritium
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- 1.Blasberg RG, Gazendam J, Patlak CS et al. (1980) Quantitative autoradiographic studies of brain edema and a comparison of multi-isotope auto-radiographic techniques. In: Cervós-Navarro J, Ferszt R (eds) Advances in neurology, vol 28: Brain edema. Raven, New York, pp 255–270Google Scholar
- 2.Bodsch W, Hossmann K-A (to be published) A quantitative regional analysis of amino acids involved in rat brain protein synthesis by high performance liquid chromatography. J Neurochem 40: 371–382Google Scholar
- 4.Bodsch W, Mies G, Hossmann K-A (to be published) Simultaneous determination of local cerebral rates of protein synthesis, glucose utilization and blood flow by multi-autoradiographic techniques. Proc Natl Acad SciGoogle Scholar
- 10.Paschen W, Djuricic B, Bosma H-J et al. (1981a) Regional blood flow and regional distribution of biochemical substrates in experimental stroke of gerbils. J Cereb Blood Flow Metabol 1 [Suppl 1]: S 174-S 175Google Scholar
- 13.Sakurada O, Kennedy C, Jehle J et al. (1978) Measurement of local cerebral blood flow with iodo (14C) antipyrine. Am J Physiol 234: H 59–66Google Scholar